DETECTION OF CARCINOGEN RESISTANT CELLS IN ORAL MUCOSA

拟议研究的具体目标是：1)识别化学物质 能有效抑制金黄地鼠颊囊袋复制的药物 角质形成细胞(HBPE细胞)体外培养，不诱导耐药细胞 急性化学物质暴露期间的变异，以及2)使用 在特定目的1中鉴定的化学试剂在体外选择，以及 从而鉴定和定量HBPE细胞的克隆 致癌过程中的一个或多个步骤。建议的化验方法 启动HBPE细胞是基于这样的假设：在启动过程中 体内，细胞获得对细胞毒作用的相对抵抗力 诱发致癌物质。这项拟议研究的长期目标是 制定改进的生物和生化策略 口腔黏膜癌前细胞的特征及其早期意义 人类口腔癌的检测和预防。

The specific aims of the proposed research are: 1) to identify chemical agents which acutely inhibit replication of hamster buccal pouch keratinocytes (HBPE cells) in vitro, and do not induce resistant cell variants during the period of acute chemical exposure, and 2) to use the chemical agents identified in Specific Aim 1 to select in vitro, and thereby identify and quantitate colonies of HBPE cells which have undergone one or more steps in the carcinogenic process. The proposed assay for initiated HBPE cell is based on the hypothesis that during initiation in vivo, cells acquire a relative resistance to the cytotoxic effects of the inducing carcinogen. The long-range goal of the proposed research is to develop improved strategies for the biological and biochemical characterization of precancerous cells in oral mucosa, and for the early detection and prevention of human oral cancer.