HYBRIDOMA ASSAYS AND RELATED LABORATORY TESTS

杂交瘤测定和相关实验室测试

• 批准号: 3604114
• 负责人: RONALD W GILLETTE
• 金额: $ 22万
• 依托单位: MELOY LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-10-01 至 1985-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3604114
• 关键词: clone cells; hybridomas; immunologic assay /test; immunoperoxidase; monoclonal antibody; neoplasm /cancer immunology; radioimmunoassay

Routine immunoperoxidase assays are to be performed on embedded tissue samples provided by the Project Officer, sectioned by the Contractor, and stained using standard ABC and PAP immunoperoxidase methods using monoclonal antibody provided by the Project Officer. Cell fusions are to be made for the purpose of hybridoma formation and monoclonal antibody generation. Cultures so generated are to be assayed for antibody production using protocols and necessary reagents supplied by the Project Officer. Samples and records are to be transported to the NIH when necessary; technical reports are to be submitted under the terms stipulated in the contract.

对包埋组织进行常规免疫过氧化物酶测定 由项目官员提供的样品，由承包商切片，以及 使用标准ABC和PAP免疫过氧化物酶方法染色， 项目官员提供的单克隆抗体。 细胞融合是 用于杂交瘤形成和单克隆抗体 一代 对如此生成的培养物进行抗体测定 使用项目提供的方案和必要试剂进行生产 警官. 样本和记录应在以下情况下运送至NIH： 必要;技术报告应根据规定的条件提交 在合同里。