REGULATION OF GENE EXPRESSION BY ONCOGENES

癌基因对基因表达的调节

• 批准号: 3093911
• 负责人: Phillip A Sharp
• 金额: $ 61.49万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-05-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3093911
• 关键词: gene expression; genetic regulation; oncogenes; virus genetics

The central theme of this program grant is regulation of gene expression by oncogenes. We will address this theme by (a) isolation of new oncogenes, (b) study of the regulation of known oncogenes, (c) study of the mechanism by which oncogenes can affect transcription of other genes, (d) definition and purification of factors which regulate promoters, and (e) identification of factors which recognize enhancer elements in a cell type specific fashion. The exciting new aspect of this theme is that part of the problem of cancer can now be phrased in the context of the action and control of known genes. At the moment, the set of oncogenes that have been identified number about 25-30. These genes are probably involved in essential cellular processes and are altered in transformed cells in terms of either level of activity or type of activity. The next frontier in research on oncogenesis is to determine how these oncogene proteins function biochemically and affect alterations in cell behavior. This is a challenging problem as little is known about the cellular processes, particularly nuclear processes, that regulate cell growth. Success in this endeavor will require the close interaction and collaboration of scientists interested in: the action of oncogenes, the intracellular signals generated by peptide hormones, the regulation of gene expression and the biochemistry of nuclear processes such as transcription. Understanding the activities of oncogenes at this molecular level will reveal how they collaborate in transformation and the relationships of transformation to processes such as differentiation

该项目资助的中心主题是通过以下方式调节基因表达： 致癌基因我们将通过（a）分离新的致癌基因，（B） 研究已知致癌基因的调节，（c）研究 哪些癌基因可以影响其他基因的转录，（d）定义和 纯化调节启动子的因子，和（e）鉴定 以细胞类型特异性方式识别增强子元件的因子。 这个主题令人兴奋的新方面是癌症问题的一部分 现在可以在已知基因的作用和控制的上下文中表达。 目前，已经确定的癌基因组数量约为 25-30.这些基因可能参与基本的细胞过程， 在转化的细胞中，在活性水平或 活动的类型。肿瘤发生研究的下一个前沿是 确定这些癌基因蛋白质如何在生物化学上起作用， 细胞行为的改变。这是一个具有挑战性的问题， 了解细胞过程，特别是核过程， 调节细胞生长。这一奋进的成功将需要密切 感兴趣的科学家的互动与合作： 癌基因，由肽激素产生的细胞内信号， 基因表达的调节和核过程的生物化学， 作为转录。了解癌基因的活动， 分子水平将揭示它们如何在转化中合作， 转化与分化等过程的关系