BRAIN PEPTIDES ON PITUITARY FUNCTIONS AND DIABETES

脑肽对垂体功能和糖尿病的影响

• 批准号: 3095181
• 负责人: ROGER C. GUILLEMIN
• 金额: $ 120.51万
• 依托单位: WHITTIER INSTITUTE FOR DIABETES & ENDOC
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3095181
• 关键词: hypothalamic pituitary axis; neuroendocrine system; neuropeptides

Part of a larger Program Project application dealing with fundamental and applied neuroendocrinology in the field of reproduction, growth, stress and diabetes. Specifically it is proposed to establish the physiology of the recently isolated hypothalmic growth hormone releasing factor, in particular to assess its activity on tissues other than the adenohypophysis such as pancreas (re. secretion of insulin and glucagon), the gut (re. secretion of VIP), the stomach (re. secretion of gastrin). To produce antisera against GRF for designing and validating a radio immunoassay to GRF and for establishing a mapping of GRF by immunohistochemistry. To synthesize large quantities of GRF for clinical studies in normal individuals and patients with juvenile or maturity onset diabetes and several types of growth problems. To characterize and synthesize the hypothalmic endorphin-corticotropin-releasing-factor; to characterize several corticotrophic factors other than ACTH, in particular a factor controlling the secretion of androgens by the adrenal cortex (androtropin). To characterize multiple forms of native somatostatin from central nervous system, pancreas and other peripheral sources. To devise new methods of analytical and structural chemistry of extremely high sensitivity eventually leading to the ability to sequence polypeptides in the range of one nanomole.

一个更大的程序项目应用程序的一部分，处理基础和 应用神经内分泌学在生殖、生长、压力和 糖尿病 具体而言，建议建立 最近分离的下丘脑生长激素释放因子，特别是 评估其对腺垂体以外的组织如胰腺的活性 （re.胰岛素和胰高血糖素的分泌），肠（re. VIP分泌）， 胃（RE。胃泌素分泌）。 为了制备抗GRF的抗血清， 设计和验证GRF的放射免疫测定，并建立 免疫组织化学法定位GRF。 合成大量的GRF 对于正常个体和青少年或 成熟期糖尿病和几种生长问题。 表征 并合成下丘脑内啡肽-促肾上腺皮质激素释放因子;以 表征除ACTH以外的几种促皮质因子，特别是 控制肾上腺皮质分泌雄激素的因子 （促雄激素）。 为了表征多种形式的天然生长抑素， 中枢神经系统、胰腺和其他外周来源。 设计新 极高灵敏度的分析和结构化学方法 最终导致在一个范围内对多肽进行测序的能力 纳摩尔。