LIVER/INTESTINAL METABOLISM OF BILE ACIDS AND LIPIDS

肝肠脂质计划项目（LILPP）包括一个 一组密切相关的研究项目，均涉及研究 胆汁酸、胆固醇、磷脂和甘油三酯 通过肝脏或肠道进行代谢和运输。 拟议 该计划项目包括七个单独的赠款申请， 独立调查员，长期以来一直对 胆固醇、胆汁酸和/或其它脂质的代谢。 四 该计划项目的成员来自 胃肠病学和其他四个来自基础学院 健康科学。 该项目的总体目标是 汇集各个调查人员的资源和才能， 为了进一步了解肝脏和肠道 脂质代谢。 这项建议的具体目标是 目的：a）阐明胆汁酸的调节机制 合成和分泌; B）确定调节 体内游离胆固醇的“代谢活性池”的大小 肝脏是胆汁酸合成的前体来源， 胆汁胆固醇排泄; c）提供动力学定义 血浆游离胆固醇和酯化胆固醇的贡献 不同脂蛋白组分对胆汁酸合成和胆汁 人的胆固醇分泌（正常受试者和患有 不同类型的高脂血症）; d）描述机制 调节磷脂和甘油三酯的合成 肝脏; e）描述激素在调节胆汁中的作用 盐在肝脏中的运输和分泌; f）确定 胆汁盐在肠道吸收Ca++和Fe++中的作用， Ca ~（++）对回肠胆盐吸收的影响 胆汁酸的肠道微生物代谢的基础研究。 细胞培养-分子生物学核心实验室 Hylemon博士的指导，将在这个项目中发挥关键作用 项目 这个核心实验室将提供现代技术， 提供给几个调查员。 LILPP的这一方面将 允许一些PI。这是一种更深入的研究方法 不同研究者之间的广泛合作研究 已经启动，其他一些正在规划中 相位

The Liver-Intestinal Lipid Program Project (LILPP) consists of a group of closely related research projects all dealing with studies of bile acids, cholesterol, phospholipid and triglyceride metabolism and transport by the liver or intestines. The proposed program project consists of seven individual grant applications by independent investigators, with long standing interests in the metabolism of sterols, bile acids and/or other lipids. Four members of the program project are from the Division of Gastroenterology and the other four are from the School of Basic Health Sciences. The overall objective of the program project is to pool the resources and talents of individual investigators in order to further our understanding of hepatic and intestinal metabolism of lipids. The specific objectives of this proposal are to: a) elucidate the mechanisms of regulation of bile acid synthesis and secretion; b) determine the factors which regulate the size of "metabolically active pool" of free cholesterol in the liver, which is the precursor source for bile acid synthesis and biliary cholesterol excretion; c) to provide a kinetic definition of the contribution of plasma free and esterified cholesterol from different lipoprotein fractions to bile acid synthesis and biliary cholesterol secretion in man (normal subjects and patients with different types of hyperlipidemia); d) to describe the mechanism of regulation of phospholipid and triglyceride synthesis in the liver; e) describe the role of hormones in the regulation of bile salt transport and secretion in the liver; f) to determine the role of bile salt in the intestinal absorption of Ca++ and Fe++ and effects of Ca++ on ileal bile salt absorption and g) to carry outbasic studies of intestinal microbial metabolism of bile acids. The Cell Culture-Molecular Biology Core laboratory, under the direction of Dr. Hylemon, will play a pivotal role in this program project. This core laboratory will offer modern technology not available to several investigators. This aspect of the LILPP will permit a number of P.I.'s a more indepth approach in their studies. Extensive collaborative studies between different investigators have already been initiated and a number of others are in planning phase.