Understanding how interaction of single-walled carbon nanotubes with biological interfaces in the gastrointestinal system may alter chemical bioavailability

约瑟夫·比塞西#1605119单壁碳纳米管是一种小型人造材料，目前正用于许多消费行业。随着使用量的增加，这些材料进入水环境的可能性也增加了。这些材料的特性使它们极有可能被鱼类等水生生物消耗，在那里它们可能与鱼的肠道系统相互作用。该项目的总体目标是了解单壁碳纳米管如何通过使鱼类更容易受到化学物质(即污染物)的影响来影响肠道环境，这些化学物质已经存在于我们的环境中，并与健康问题有关。为了实现这一目标，PI将研究单壁碳纳米管如何改变暴露在水中的鱼类肠道的正常结构和功能，并确定这些变化是否有助于增加它们对我们水生环境中已经存在的其他化学物质的敏感性。单壁纳米管在性质上是亲脂性的，很可能吸收我们环境中已经存在的其他水生污染物，并通过饮食暴露途径改变鱼类的肠道。本研究的目的是为了更好地了解单壁碳纳米管与鱼肠的特异性相互作用，通过探讨它们与胃肠道系统中的脂类物质的相互作用，从而可能导致肠道脂质体的结构和功能的变化。PI还将检查胃肠道脂质谱的这种变化是否会导致可能被吸附到单壁碳纳米管上的化学物质的释放，从而增加这些污染物的生物积累。PI将通过检测单壁碳纳米管饮食暴露期间的胃肠道脂质分布，确定脂类对这些材料对化学物质吸附的影响，以及检测纳米脂类相互作用对化学生物积累的影响来实现这些目标。该项目开展的活动预计将在国际、国家和区域范围内产生深远影响。纳米生物相互作用研究的结果将有助于下一代纳米材料的设计，这些材料利用或避免已确定的下游行为。通过这种方式，PI可以继续利用纳米材料的惊人特性，而不会产生有害的后果。在地方一级，研究小组计划将代表人数不足的群体的学生纳入该项目，并通过使用视频增加公众对纳米材料好处的了解。科学界将通过在区域会议上教授短期课程以及在国家会议上举行领导会议，在全国范围内参与这一项目。最后，研究小组将通过国际社区现有的教学计划，整合有关纳米材料用于应对资源匮乏国家健康问题的信息，从而扩大教育。

Bisesi, Joseph H.# 1605119 Single-walled carbon nanotubes are small man-made materials that are currently being used in a number of consumer industries. With this increased use, there is increased likelihood that these materials will make their way into aquatic environments. The materials have properties which make them highly likely to be consumed by aquatic organisms such as fish where they may interact with the fish intestinal system. The overall objective of this project is to understand how single-walled carbon nanotubes influence the intestinal environment by making fish more susceptible to chemicals (i.e. pollutants) that are already present in our environment and are associated with health concerns. To achieve this objective the PI will examine how single-walled carbon nanotubes may change the normal structure and function of the intestines of exposed fish and determine whether these changes contribute to increasing their susceptibility to other chemicals that are already present in our aquatic environments. Single-walled nanotubes are lipophilic in nature and are likely to sorb other aquatic contaminants already present in our environment as well as alter the intestinal tract of fish through dietary exposure routes. The objective of this research is to better understand the specific interactions of single-walled carbon nanotubes with fish intestines by probing their interaction with lipids in the gastrointestinal system which may result in structural and functional changes in the intestinal lipidome. The PI will also examine whether such changes in gastrointestinal lipid profiles result in release of chemicals that may be adsorbed to single-walled carbon nanotubes, increasing bioaccumulation of these contaminants. The PI will accomplish these objectives by examining gastrointestinal lipid profiles during single-walled carbon nanotube dietary exposure, determining the effects of lipids on sorption of chemicals to these materials, and examining the effects of nano-lipid interactions on chemical bioaccumulation. Activities performed in this project are expected to have far reaching impacts on an international, national, and regional scale. Results from the nano-bio interaction studies will contribute to the design of next generation nanomaterials that take advantage of, or avoid, identified downstream behavior. In this way the PI can continue to utilize the amazing properties of nanomaterials without detrimental consequences. On a local level, the research team plans to include students from underrepresented groups in this project as well as increasing public knowledge on the benefits of nanomaterials through the use of videos. The scientific community will be involved in this project on a national scale through teaching of short courses at regional meetings as well as leading sessions at national meetings. Finally, the research team will expand education by integrating information about nanomaterial uses for combating health issues in resource poor countries through existing teaching programs in international communities.