PROGRAM OF EXCELLENCE IN MOLECULAR BIOLOGY

许多心血管和肺部疾病与细胞紊乱有关。 增殖分化。卓越的分子课程 加州大学旧金山分校的生物学将通过利用两个 我们机构的最大优势--一群杰出的分子 对分子生物学做出显著贡献和 心血管研究所一直致力于研究 心血管和肺部生物学在过去的三十年里。通过 将这些团体聚集在一起，是过去三十年的计划。通过 将这些小组聚集在一起，卓越计划将是 加州大学旧金山分校心血管生物学新方向的焦点 这项提议的科学目标是使用最复杂的 分子和发育生物学方法来确定关键 调节心脏生长和分化的分子， 血管和肺组织，研究其结构和功能。 这些分子处于正常发育和疾病中：为了发现 前体细胞和细胞相互作用负责 心脏和血管的形成。调查的主题将 包括生长因子及其受体在血管生成中的作用 和心脏生成，分子功能的结构基础 参与心血管和肺的发育，调节 肺发育过程中基因表达及细胞周期的鉴定 控制基因及其在增殖性疾病中的作用和功能 肌原纤维心脏蛋白的胚胎亚型。在技能上 发展计划年轻调查人员将接受技术指导 比如通过“基因打靶”创造出转基因小鼠。 利用胚胎干细胞中的同源重组，表达和 消减cDNA克隆，X射线结晶学，原位杂交， 定点诱变和饱和诱变，以及大规模应用 蛋白质表达系统。他们还将学习新的方法 发展生物学。卓越计划将建立一个 独特的高级调查员计划，将支持年轻人 博士后培训结束后的调查人员。因此，程序将 为新的研究方向和发展提供机会 将产生新一代的分子生物学家 心血管和肺部生物学。

Many cardiovascular and pulmonary disease involve disorders of cell proliferation and differentiation. A program of Excellence in Molecular Biology at U.C.S.F. would focus on these problems by utilizing two of the greatest strengths of our institution - an outstanding group of molecular biologists who have made notable contributions to molecular biology and a Cardiovascular Research Institute that has been dedicated to the study of cardiovascular and pulmonary biology for the last three decades. By bringing these groups together, the Program of the last three decades. By bringing these grouwps together, the Program of Excellence would be the focus for new directions in cardiovascular biology at U.C.S.F. The scientific goals of this proposal are to use the most sophisticated molecular and developmental biology approaches to identify the key molecules that regulate the growth and differentiation of cardiac, vascular and pulmonary tissues, to study the structure and function of these molecules in normal development and in disease: and to discover the precursor cells and cellular interactions that are responsible for formation of the heart and blood vessels. Topic of investigation will include the role of growth factors and their receptors in angiogenesis and cardiogenesis, the structural basis for the function of molecules involved in cardiovascular and pulmonary development, the regulation of gene expression in the developing lung, the identification of cell cycle control genes and their role in proliferative diseases and the function of embryonic isoforms of myofibrillar cardiac proteins. In the Skills Development Program young investigators will be instructed in techniques such as the creation of genetically altered mice by "gene targeting" using homologous recombination in embryonic stem cells, expression and subtractive cDNA cloning, x-ray crystallography, in situ hybridization, site-directed and saturation mutagenesis, and the use of large scale systems for expression of proteins. They will also learn novel approaches to developmental biology. The Program of Excellence will establish a unique advanced investigator program that will support young investigators after their postdoctoral training. Thus the Progrram will provide the opportunity for development of new research directions and will produce a new generation of molecular biologists workintg in cardiovascular and pulmonary biology.