PROGRAM OF EXCELLENCE IN MOLECULAR BIOLOGY

许多心血管和肺部疾病都涉及细胞功能紊乱， 增殖和分化。分子生物学卓越计划 U.C.S.F.的生物学将通过利用其中的两个来关注这些问题。 我们机构的最大优势-一个杰出的分子组 对分子生物学做出了显著贡献的生物学家， 心血管研究所一直致力于研究 心血管和肺生物学的研究。通过 把这些团体聚集在一起，过去三十年的计划。通过 将这些团体聚集在一起，卓越计划将是 在U.C.S.F.心血管生物学的新方向的焦点。 这项提议的科学目标是使用最先进的 分子和发育生物学方法来确定 调节心脏细胞生长和分化的分子， 血管和肺组织，研究结构和功能， 这些分子在正常发育和疾病中的作用： 前体细胞和细胞间的相互作用 心脏和血管的形成。调查主题将 包括生长因子及其受体在血管生成中的作用 和心脏发生，分子功能的结构基础 参与心血管和肺的发育， 发育中肺的基因表达，细胞周期的鉴定 控制基因及其在增殖性疾病中的作用和功能 肌原纤维心脏蛋白的胚胎亚型。在技能 发展计划年轻的研究人员将在技术指导 例如通过"基因靶向"来制造基因改变的老鼠 利用胚胎干细胞中的同源重组， 消减cDNA克隆，X射线晶体学，原位杂交， 定点和饱和诱变，以及使用大规模 蛋白质表达系统。他们还将学习新的方法 发展生物学。卓越计划将建立一个 独特的高级研究员计划，将支持年轻的 经过博士后培训的研究人员。因此，该计划将 为发展新的研究方向提供机会， 将产生新一代的分子生物学家， 心血管和肺生物学。

Many cardiovascular and pulmonary disease involve disorders of cell proliferation and differentiation. A program of Excellence in Molecular Biology at U.C.S.F. would focus on these problems by utilizing two of the greatest strengths of our institution - an outstanding group of molecular biologists who have made notable contributions to molecular biology and a Cardiovascular Research Institute that has been dedicated to the study of cardiovascular and pulmonary biology for the last three decades. By bringing these groups together, the Program of the last three decades. By bringing these grouwps together, the Program of Excellence would be the focus for new directions in cardiovascular biology at U.C.S.F. The scientific goals of this proposal are to use the most sophisticated molecular and developmental biology approaches to identify the key molecules that regulate the growth and differentiation of cardiac, vascular and pulmonary tissues, to study the structure and function of these molecules in normal development and in disease: and to discover the precursor cells and cellular interactions that are responsible for formation of the heart and blood vessels. Topic of investigation will include the role of growth factors and their receptors in angiogenesis and cardiogenesis, the structural basis for the function of molecules involved in cardiovascular and pulmonary development, the regulation of gene expression in the developing lung, the identification of cell cycle control genes and their role in proliferative diseases and the function of embryonic isoforms of myofibrillar cardiac proteins. In the Skills Development Program young investigators will be instructed in techniques such as the creation of genetically altered mice by "gene targeting" using homologous recombination in embryonic stem cells, expression and subtractive cDNA cloning, x-ray crystallography, in situ hybridization, site-directed and saturation mutagenesis, and the use of large scale systems for expression of proteins. They will also learn novel approaches to developmental biology. The Program of Excellence will establish a unique advanced investigator program that will support young investigators after their postdoctoral training. Thus the Progrram will provide the opportunity for development of new research directions and will produce a new generation of molecular biologists workintg in cardiovascular and pulmonary biology.