INTEGRATIVE MECHANISMS IN LAMPREY SPINAL CORD

七鳃鳗脊髓的整合机制

• 批准号: 3901746
• 负责人: BURGESS N. CHRISTENSEN
• 金额: --
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3901746
• 关键词: Agnatha; aminoacid; dendrites; electrophysiology; interneurons; ion transport; membrane permeability; neural transmission; neuroanatomy; neurotransmitter metabolism; saltwater environment; spinal cord; voltage /patch clamp

We propose to accomplish the following specific goals. (1) To measure the wide band impedance of lamprey spinal giant interneurons using a frequency domain analysis in which the neuron is stimulated with a white noise signal and the impedance (under current clamp conditions) is measured as a function of frequency. (2) To measure the wide band impedance of giant interneurons under conditions in which the cell membrane conductance is changed by membrane polarizaion brought about by current injection, activation of excitatory and inhibitory synapitc input, application of putative transmitters at specific identified dendritic sites and during regeneration following spinal cord injury. (3) To measure the cable parameters of these cells using independent methods. The first will utilize the data obtained from the wide band impedance measurements. These data will be curve fit with a model from which the membrane time constant, dendritic to soma conductance ratio, and dendritic electron length can be estimated. The second will be from morphological measurements of dendritic lengths and diameters from HRP labeled cells. The research outlined in this proposal has as its aim to examine the hypothesis that neuronal integration of synaptic potentials depends on the dynamic membrane properties of the dendritic structure.

我们建议实现以下具体目标。 (1) 至 测量七鳃鳗脊椎巨人的宽带阻抗 中间神经元使用频域分析，其中 神经元受到白噪声信号的刺激，并且阻抗 （在电流钳条件下）被测量为函数 频率。 (2) 测量巨型器件的宽带阻抗 中间神经元在细胞膜 膜极化引起的电导发生变化 通过电流注入，激活兴奋性和抑制性 synapitc 输入，假定发射机在特定的应用 确定树突位点并在脊髓后再生过程中 脊髓损伤。 (3) 测量这些电池的电缆参数 使用独立的方法。 第一个将利用数据 从宽带阻抗测量中获得。 这些 数据将与膜的模型进行曲线拟合 时间常数、树突与体细胞电导率比和树突 可以估计电子长度。 第二个将来自 树突长度和直径的形态测量 来自 HRP 标记的细胞。 本提案中概述的研究的目的是检查 突触电位的神经元整合假说 取决于树突的动态膜特性 结构。