ROCKY MOUNTAIN TASTE AND SMELL CENTER

本提案要求为该计划提供补充资金 支持落基山口味和美食的kGrant项目 中心 调查员都是除了Kinnamon博士， 成员住在同一栋大楼内的大学 位于科罗拉多州丹佛市的科罗拉多医学院。 该中心将 由五个主要的实验室和人员组成， 调查人员，相互共享的核心设施系统，以及 大学医院的耳鼻喉科诊所 毗邻医学院。 的主要目标 建议的计划项目是增加基础知识 以及对感官的神经生物学基础的理解 以及它们在细胞水平上的紊乱。的 目标将通过一系列五个主要方面来实现 1）脊椎动物嗅觉的生物医学研究 粘膜 2)脊椎动物味蕾中的化学感受传导。 3)电生理学鉴定的解剖学分析 初级味觉传入 4)正常和异常的神经生物学 人类味觉感受器 5)脊椎动物味觉的超微结构 芽 这些调查将利用一些强大的新的 比如味觉感受器的细胞内记录 和计算机生成的三维结构分析 嗅觉和味觉感受器的高压电子显微照片， 举两个例子--阐明味觉的细胞神经生物学 和嗅觉受体。 在科罗拉多大学我们很幸运 拥有优良的设备、设施、充足的实验室和诊所 空间和人员已经到位。 五位主要调查员 都是经过各种专业培训的合格的生物医学科学家， 神经生物学学科包括电子显微镜， 电生理学、神经解剖学、外科学和细胞生物学。

The present proposal request supplemental funds for the Program Project kGrant that supports the Rocky Mountain Taste and Smell Center. The investigators are all save Dr. Kinnamon, Faculty members housed within the same building in the University of Colorado School of Medicine in Denver, Colorado. The Center will consist of the laboratories and personnel of the five principal investigators, a system of mutally shared Core facilities, and the Otorhinolaryngology clinic at University Hospital directly adjacent to the School of Medicine. The major objective of the proposed Program Project is to increase the fundamental knowledge and understanding of the neurobiological substrates of the senses of taste and smell and their disorders at the cellular level. That objective will be accomplished through a series of five major projects; 1) Biomedical investigations of vertebraqte olfactory mucosa. 2) Chemosensory transduction in vertebrate taste buds. 3) Anatomical analysis of electrophysiologically identified primary gustatory afferents. 4)Neurobiology of normal and abnormal human gustatory receptors. 5) Ultrastructure of vertebrate taste buds. These investigations will utilize a number of powerful new approaches--such as intracellular recording from taste receptors and computer-generated three-dimensional structure analysis of high voltage electron micrographs of olfactory and taste receptors, to name but two--to elucidate the cellular neurobiology of taste and smell receptors. At the University of Colorado we are fortunate to have excellent equipment, facilities, ample laboratory and clinic space, and personnel already in place. The five principal investigators are all qualified biomedical scientists trained in a variety of major neurobiological disciplines including electron microscopy, electrophysiology, neuroanatomy, surgery, and cell biology.