ROCKY MOUNTAIN TASTE AND SMELL CENTER

本提案要求为方案项目提供补充资金 格兰特，目前支持落基山品尝和烹饪中心。 除了金纳蒙博士，所有的调查人员都住在 同样的建筑在科罗拉多大学医学院， 丹佛，科罗拉多。 该中心将由实验室和 五个主要研究者的人员，一个相互共享的系统， 核心设施，以及大学医院的耳鼻喉科诊所 紧邻医学院。 的主要目标 建议的计划项目是增加基础知识， 理解味觉的神经生物学基础， 嗅觉及其在细胞水平上的紊乱。 这一目标将是 通过一系列五大项目完成：1）生物医学 脊椎动物嗅粘膜的研究，大卫T. Moran，P.I.; 2）脊椎动物味蕾中的化学感觉转导，Stephen D.罗珀， P.I.; 3)电生理学鉴定原发性肝癌的解剖学分析 味觉传入，托马斯E.芬格，私家侦探; 4)神经生物学正常和 异常的人类味觉受体，布鲁斯W. Jafek，P.I.;和 5）脊椎动物味蕾的超微结构，John C. Kinnamon，P.I. 这些 调查将利用一些强有力的新方法，如 味觉感受器的细胞内记录 高压电子显微照片的三维结构分析 嗅觉和味觉受体，仅举两个例子--为了阐明 味觉和嗅觉受体的细胞神经生物学。 大学的 科罗拉多，我们很幸运，有优良的设备，设施， 实验室和诊所空间，以及已经到位的人员。 五 主要研究人员都是非常合格的生物医学 接受过各种主要神经生物学学科培训的科学家 包括电子显微镜，电生理学，神经解剖学，外科手术， 和细胞生物学。 调查人员之间的相互关系， 调查和共享设施是如此接近，在目前， 计划项目，整体将出现远远大于其总和 零部件

The present proposal requests supplemental funds for the Program Project Grant that presently supports the Rocky Mountain Taste and Smell Center. The investigators are all, save Dr. Kinnamon, Faculty members housed within the same building in the University of Colorado School of Medicine in Denver, Colorado. The Center will consist of the laboratories and personnel of the five principal investigators, a system of mutually shared Core facilities, and the Otorhinolaryngology clinic at University Hospital directly adjacent to the School of Medicine. The major objective of the proposed Program Project is to increase the fundamental knowledge and understanding of the neurobiological substrates of the senses of taste and smell and their disorders at the cellular level. That objective will be accomplished through a series of five major projects: 1) Biomedical investigations of vertebrate olfactory mucosa, David T. Moran, P.I.; 2)\Chemosensory transduction in vertebrate taste buds, Stephen D. Roper, P.I.; 3) Anatomical analysis of electrophysiologically identified primary gustatory afferents, Thomas E. Finger, P.I.; 4) Neurobiology of normal and abnormal human gustatory receptors, Bruce W. Jafek, P.I.; and 5)\Ultrastructure of vertebrate taste buds, John C. Kinnamon, P.I. These investigations will utilize a number of powerful new approaches--such as intracellular recording from taste receptors and computer-generated three-dimensional structure analysis of high voltage electron micrographs of olfactory and taste receptors, to name but two--to elucidate the cellular neurobiology of taste and smell receptors. At the University of Colorado we are fortunate to have excellent equipment, facilities, ample laboratory and clinic space, and personnel already in place. The five principal investigators are all exceptionally well-qualified biomedical scientists trained in a variety of major neurobiological disciplines including electron microscopy, electrophysiology, neuroanatomy, surgery, and cell biology. The interrelationship between the investigators, investigations, and shared facilities is so close that, in the present Program Project, the whole will emerge far greatr than the sum of its component parts.