MORPHOLOGY AND FUNCTION OF THE BASAL GANGLIA

该计划的目标是研究其功能和 使用各种技术和方法发展基底节 准备工作。这项提议的力量是从 在六个实验室中每一个都建立了工作，其负责人 调查人员拥有不同的背景，代表着各个领域 解剖学、电生理学、药理学、分子和细胞学 生物学和行为学。这些调查人员由一个 共同的原因；即理解功能和 多学科和多学科研究下基底神经节的发育 高度互动的研究项目。我们计划调查一项) 脑内突触的发育及其顺序和地形图 新纹状体，b)递质的发育和形态 纹状体神经元的表型及其特征 它们的离子电导，c)假定的发射器对 基底节神经元，d)新的假定的鉴定 苍白球中的神经递质，e)单位活动 在清醒动物的基底节中，以及f)脑移植在 基底节。 项目1将打算在初级解体中刻画 大鼠组织块培养中神经递质和神经递质的表达 免疫细胞化学技术在形态表型中的应用 结合光学和电子显微镜，离子 全电池和单通道电压钳位的电导 技术，以及多巴胺对这些神经元的电生理效应 神经元。项目2将研究多巴胺和 乙酰胆碱在大鼠纹状体脑片制备中的应用 细胞内记录技术与形态关系 在多巴胺传入和纹状体神经元之间。项目3研究 运动过程中纹状体神经元的放电模式 灵长类动物中由感觉触发的运动任务。伴随而来的 解剖学研究，使用免疫细胞化学示踪结合 提出了用光镜和电子显微镜观察。项目4将 确定正常细胞连接的程度 纹状体移植物中的排列是通过细胞内复制的 结合光的记录法和PHA-L示踪法 和电子显微镜。

The goal of this program is to investigate the function and development of the basal ganglia using a variety of techniques and preparations. The strength of the proposal has been built from established work in each of six laboratories, whose principal investigators possess diverse backgrounds representing the fields of anatomy, electrophysiology, pharmacology, molecular and cell biology, and behavior. These investigators are united by a common cause; namely to understand the function and development of the basal ganglia under a multidisciplinary and highly interactive research program. We plan to investigate a) development of synapses and order and topography in the neostriatum, b) development of transmitter and morphological phenotype of the striatal neurons and the characterization of their ionic conductances, c) action of putative transmitters on the basal ganglia neurons, d) identification of new putative neurotransmitters in the globus pallidum, e) single-unit activities in the basal ganglia of awake animals, and f) brain graft in the basal ganglia. Project 1 will intend to characterize in the primary dissociated rat explant culture the expression of neurotransmitter and morphological phenotypes using immunocytochemical techniques in conjunction with light and electronmicroscopy, ionic conductances by whole-cell and single-channel voltage clamp techniques, and electrophysiological effects of dopamine on these neurons. Project 2 will examine the action of dopamine and acetylcholine in in vivo rats striatal slice preparation by the intracellular recording technique and morphological relationship between dopamine afferents and striatal neurons. Project 3 study the firing pattern of striatal neurons during the performance of a sensory-triggered movement tasks in the primate. Concomitant anatomical studies, using immunocytochemical tracing combined with light and electronmicroscope are proposed. Project 4 will determine the extent to which the normal cellular connectional arrangements are reproduced in striatal graft by intracellular recording and PHA-L tracing methods in conjunction with light and electron microscopy.