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Dissemination of MAPseq and BARseq for high-throughput brain mapping

传播用于高通量脑图谱的 MAPseq 和 BARseq

基本信息

批准号：
10440138
负责人：
ANTHONY M ZADOR
金额：
$ 109.59万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2027-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10440138
关键词：
Age Anatomy Animal Model Animals Area Atlases Axon Bar Codes Biological Models Brain Brain Mapping Brain region Carrier Proteins Collaborations Communities Complex Computer software Custom DNA sequencing Data Development Distant Engineering Foundations Gender Gene Expression Generations Genes Genetic Models Gilles de la Tourette syndrome Goals High-Throughput DNA Sequencing In Situ Infection Interneurons Label Laboratories Libraries Maps Mental Depression Methods Multiplexed Analysis of Projections by Sequencing Mus Neocortex Neuroanatomy Neurofibrillary Tangles Neurons Neuropil Neurosciences Neurosciences Research Population Presynaptic Terminals Probability Protocols documentation Publications Publishing RNA Reagent Resolution Reverse Transcription Role Schizophrenia Scientist Specificity Standardization Synapses Techniques Technology Time Training and Education Viral Virus Vision Work addiction autism spectrum disorder base bioinformatics pipeline brain tissue cost in situ sequencing insight interest neuronal circuitry neuropsychiatric disorder next generation next generation sequencing novel novel strategies relating to nervous system skills tissue processing transcriptomics user-friendly

项目摘要

The goal of this project is to disseminate MAPseq and BARseq to the broader neuroscience community. These are novel methods developed in my laboratory based on high-throughput DNA sequencing for determining neuronal circuitry. Neurons transmit information to distant brain regions via long-range axonal projections. In some cases, functionally distinct populations of neurons are intermingled within a small region. Disruptions of connectivity may underlie many neuropsychiatric disorders including autism and schizophrenia. At present, neuroanatomical techniques—particularly those with single neuron resolution—are expensive and labor intensive. MAPseq and BARseq convert neuroanatomy into a problem of DNA sequencing, and thereby allow us to exploit the tremendous breakthroughs in next- generation sequencing throughput. The dissemination of these high-throughput methods for determining neuronal projections will have important implications for understanding normal neuronal circuitry, and how this circuitry is disrupted in animal models of neuropsychiatric disorders like autism and schizophrenia.

该项目的目标是将MAPseq和BARseq传播给更广泛的神经科学社区。这些都是我的实验室基于高通量DNA开发的新方法用于确定神经元回路的测序。神经元将信息传递给遥远的大脑通过长距离轴突投射的区域。在某些情况下，功能上不同的种群神经元在一个很小的区域内混合在一起。连接中断可能是许多包括自闭症和精神分裂症在内的神经精神障碍。目前，神经解剖学技术--特别是那些具有单个神经元分辨率的技术--是价格昂贵，劳动密集型。MAPseq和BARseq将神经解剖学转化为 DNA测序，从而允许我们利用下一步的巨大突破- 生成序列吞吐量。这些高通量方法的传播确定神经元投射将对理解正常具有重要意义神经回路，以及在神经精神病学动物模型中这种回路是如何被破坏的自闭症和精神分裂症等疾病。