Dissemination of MAPseq and BARseq for high-throughput brain mapping

该项目的目标是将MAPseq和BARseq传播到更广泛的神经科学领域。 社区这些是我实验室开发的基于高通量DNA的新方法 用于确定神经元回路的测序。神经元将信息传输到远处的大脑 区域通过长距离轴突投射。在某些情况下，功能上不同的群体， 神经元混杂在一个小区域内。连接中断可能是许多 神经精神障碍，包括自闭症和精神分裂症。 目前，神经解剖学技术-特别是那些具有单神经元分辨率的技术- 昂贵且劳动密集。MAPseq和BARseq将神经解剖学转化为一个问题， DNA测序，从而使我们能够利用巨大的突破，在未来- 代测序通量。这些高通量方法的传播， 确定神经元投射将对理解正常的 神经元回路，以及这种回路是如何在神经精神疾病的动物模型中被破坏的。 像自闭症和精神分裂症。

The goal of this project is to disseminate MAPseq and BARseq to the broader neuroscience community. These are novel methods developed in my laboratory based on high-throughput DNA sequencing for determining neuronal circuitry. Neurons transmit information to distant brain regions via long-range axonal projections. In some cases, functionally distinct populations of neurons are intermingled within a small region. Disruptions of connectivity may underlie many neuropsychiatric disorders including autism and schizophrenia. At present, neuroanatomical techniques—particularly those with single neuron resolution—are expensive and labor intensive. MAPseq and BARseq convert neuroanatomy into a problem of DNA sequencing, and thereby allow us to exploit the tremendous breakthroughs in next- generation sequencing throughput. The dissemination of these high-throughput methods for determining neuronal projections will have important implications for understanding normal neuronal circuitry, and how this circuitry is disrupted in animal models of neuropsychiatric disorders like autism and schizophrenia.