PILOT STUDY--PAIRED HELICAL FILAMENT BINDING PROTEINS

试点研究——配对螺旋丝结合蛋白

• 批准号: 3802732
• 负责人: GEORGE PERRY
• 金额: --
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3802732
• 关键词: autoradiography; binding proteins; cow; cytoskeleton; electron microscopy; hippocampus; human tissue; laboratory rat; microfilaments; microtubule associated protein; microtubules; neurofibrillary tangles; neurofilament; paired helical filament; polymerization; tau proteins

Although many studies have addressed the issue of the cellular components present in PHF none has defined the relationship of these components to PHF structure nor to each other. This lack of knowledge has resulted from a focus on components rather than their relationship. Further it has suffered from the necessity of a hypothesis with regard to likely components before a putative PHF component is examined. In the present proposal, an in vitro model system is developed utilizing the properties of polymers to define the relationship of identified PHF components as well as opening the possibility for identification of new PHF components. The utility of this approach is the possibility of assigning PHF components to a minimum of two classes, core or associated proteins, by non-immunochemical methods. PHF components detected by this protocol, but not identifiable by immunochemical approaches, will be defined by amino acid sequencing. These studies will provide an under standing of PHF as polymers current to that known for the most prevalent cellular linear polymer, the cytoskeleton.

尽管许多研究已经解决了细胞的问题