ALTERED NAC1 TRANSPORT IN HENLE'S LOOP IN--MODELS OF HYPERTENSION

高血压模型中亨利氏环中 NAC1 转运的改变

• 批准号: 3855124
• 负责人: JACK WORK
• 金额: --
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3855124
• 关键词: denervation; disease /disorder model; electrolyte balance; hormone regulation /control mechanism; ion transport; prostaglandins; renal cortex; renal hypertension; renal medulla; renal tubular transport; sodium chloride; spontaneous hypertensive rat; vasopressins

This project will focus on identifying the intrarenal site or sites of altered sodium reabsorption in two well established models of experimental hypertension. Studies will utilize the method of perfusing isolated tubule segments of specific pathogen-free Sprague-Dawley rats, Dahl S and R rats, and Brattleboro rats with diabetes insipidus. Although these animal models have been extensively studied previously utilizing other research techniques, the application of the isolated tubule technique in the rat has been limited. This new development will allow direct correlation of results of in vitro perfusion with previous results from in vivo micropuncture of the same animal model of hypertension. Methodologies will involve measurement of net and unidirectional ion fluxes and transepithelial voltages in isolated, perfused segments of the loop of Henle in the hypertensive models. Studies will directly evaluate the effect of renal denervation on sodium chloride transport in the DOCA-salt model, will examine the effect of Dahl S plasma on salt transport in both normal and Dahl distal nephron segments, and will examine the sites of action by which DOCA and vasopressin effect salt transport in the Brattleboro rat. These studies should provide information concerning the site or sites in the distal nephron that may contribute to altered sodium transport in these experimental models of hypertension.

本项目将重点确定肾内部位或部位 钠重吸收的改变，在两个良好建立的模型， 实验性高血压 研究将采用以下方法： 灌注无特定病原体的分离的小管段 Sprague-Dawley大鼠、Dahl S和R大鼠以及Brattleboro大鼠 尿崩症 尽管这些动物模型已经被 以前广泛研究利用其他研究 技术，应用离体小管技术， 老鼠是有限的。 这一新的发展将使直接 体外灌注结果与既往结果的相关性 来自相同动物模型的体内微穿刺， 高血压 方法学将涉及净收入的计量 和单向离子流和跨上皮电压 孤立的，灌注段的亨利环在 高血压模型。 研究将直接评估 去肾神经支配对DOCA盐中氯化钠转运的影响 模型，将检查达尔S等离子体对盐的影响 正常和Dahl远端肾单位段的转运，以及 将检查DOCA和加压素的作用部位， 影响布拉特伯勒大鼠的盐转运。 这些研究应 提供关于远端部位的信息 肾单位，可能有助于改变钠转运在这些 高血压的实验模型