ALTERED NAC1 TRANSPORT IN HENLE'S LOOP IN--MODELS OF HYPERTENSION

高血压模型中亨利氏环中 NAC1 转运的改变

• 批准号: 3876195
• 负责人: JACK WORK
• 金额: --
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3876195
• 关键词: denervation; disease /disorder model; electrolyte balance; hormone regulation /control mechanism; ion transport; prostaglandins; renal cortex; renal hypertension; renal medulla; renal tubular transport; sodium chloride; spontaneous hypertensive rat; vasopressins

This project will focus on identifying the intrarenal site or sites of altered sodium reabsorption in two well established models of experimental hypertension. Studies will utilize the method of perfusing isolated tubule segments of specific pathogen-free Sprague-Dawley rats, Dahl S and R rats, and Brattleboro rats with diabetes insipidus. Although these animal models have been extensively studied previously utilizing other research techniques, the application of the isolated tubule technique in the rat has been limited. This new development will allow direct correlation of results of in vitro perfusion with previous results from in vivo micropuncture of the same animal model of hypertension. Methodologies will involve measurement of net and unidirectional ion fluxes and transepithelial voltages in isolated, perfused segments of the loop of Henle in the hypertensive models. Studies will directly evaluate the effect of renal denervation on sodium chloride transport in the DOCA-salt model, will examine the effect of Dahl S plasma on salt transport in both normal and Dahl distal nephron segments, and will examine the sites of action by which DOCA and vasopressin effect salt transport in the Brattleboro rat. These studies should provide information concerning the site or sites in the distal nephron that may contribute to altered sodium transport in these experimental models of hypertension.

该项目将侧重于识别肾内部位 两个完善的模型中钠重吸收的改变 实验性高血压。 研究将利用以下方法 灌注无特定病原体的分离小管段 Sprague-Dawley 大鼠、Dahl S 和 R 大鼠以及 Brattleboro 大鼠 患有尿崩症。 尽管这些动物模型已 先前利用其他研究进行了广泛研究 技术，离体管技术的应用 老鼠受到了限制。 这一新的发展将允许直接 体外灌注结果与既往结果的相关性 来自同一动物模型的体内微穿刺 高血压。 方法论将涉及净测量 和单向离子通量和跨上皮电压 亨利环中分离的、灌注的片段 高血压模型。 研究将直接评估效果 肾去神经支配对 DOCA 盐中氯化钠转运的影响 模型，将检查 Dahl S 血浆对盐的影响 在正常和 Dahl 远端肾单位段中的运输，以及 将检查 DOCA 和加压素的作用位点 影响布拉特尔伯勒大鼠的盐转运。 这些研究应该 提供有关远端站点或站点的信息 肾单位可能有助于改变这些细胞中的钠转运 高血压实验模型。