Raman spectroscopy as a novel analytical bioprocessing tool for PAT

以蛋白质为基础的生物制药越来越广泛地用作治疗剂和生物治疗药物；例如，抗体疗法变得非常流行，用于治疗显性疾病，帮助免疫系统。这些蛋白质是在细胞内制造的，为了使它们具有活性，它们需要以正确的方式生产和纯化。虽然有些方法确实存在，但它们很费力，而且会破坏样品。相比之下，我们将开发一种基于光与物质相互作用的方法，这将使一种非常强大且非侵入性的分析成为可能。当光照射到某种物质上时，它会发生散射，有时这种光会以不同的波长散射；这种效应被称为拉曼散射。因此，我们将开发拉曼光谱作为直接原位分析生产过程的新方法，从而允许对这些基于蛋白质的生物制药进行更严格的评估。

Protein-based biopharmaceuticals are becoming increasingly widely used as therapeutic agents and biotherapeutics; for example antibody therapy is become very popular and is used for explicit diseases and helps the immune system. These proteins are manufactured within cells and in order for them to be active they need to be produced and purified in the correct way. Whilst some methods do exist they are laborious and destroy the sample. By contrast we will develop a method based on the interaction of light with matter that will enable a very powerful and non-invasive analysis. One process that happens when light is shone at a substance is that it is scattered and sometimes this light is scatter at a different wavelength; an effect named Raman scattering. We will therefore develop Raman spectroscopy as a novel method for the direct in situ analysis of the production process, thereby allowing more rigorous assessment of these protein-based biopharmaceuticals.