Complementary ex vivo multimodal optical imaging and in vivo Raman spectroscopy to understand tissue dynamics
补充离体多模态光学成像和体内拉曼光谱以了解组织动力学
基本信息
- 批准号:10683232
- 负责人:
- 金额:$ 66.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-08-15 至 2026-05-31
- 项目状态:未结题
- 来源:
- 关键词:ActinsAffectAlprostadilBiochemicalBiochemical MarkersBiochemistryBiologicalBiological AssayBiological SciencesBiomechanicsBiomedical EngineeringBloodBlood VesselsCarbocyaninesCardiacCervicalCervical RipeningCervix IncompetenceCervix UteriClinicalCollagenComplexCustomDetectionDevelopmentDyesEarly DiagnosisEarly treatmentElasticityElastinEtiologyExtracellular MatrixExtracellular Matrix ProteinsFiber OpticsFingerprintFreeze DryingGoalsHealthHemeHormonalHumanHyaluronic AcidHydration statusImageImaging TechniquesImmunohistochemistryImpairmentInfectionInterdisciplinary StudyLabelLengthLightLipidsLongitudinal StudiesMapsMeasuresMechanicsMedicalMicroscopeMicroscopyMifepristoneModelingMolecularMolecular ConformationMonitorMulti-modal optical imagingMusNeonatal MortalityOpticsOutcomePerfusionPerinatalPhasePhenotypePhysiologicalPhysiologyPregnancyPremature BirthPreparationProcessPropertyProteinsProteoglycanRaman Spectrum AnalysisReportingReproductionResearchResearch Project GrantsStructural ProteinSyndromeSystemTechniquesTechnologyTerm BirthTestingTimeTissue imagingTissuesUnited StatesUterusVascularizationVisualizationWaterWorkbiomechanical testcervical remodelingdata fusiondecorindesignex vivo imagingimprovedin vivoin vivo monitoringin vivo optical imaginginfant morbidityinfant morbidity/mortalityinnovationinsightmicroscopic imagingmorphometrymouse modelmultimodalityneonatal morbiditynoveloptical fiberphysical sciencepregnantprematureprototyperesponsetissue mapping
项目摘要
PROJECT SUMMARY
Limited approaches exist to examine longitudinal changes in the structural and biochemical properties of tissues
under various physiological conditions such as pregnancy. Light and light based technologies offer the potential
for non-invasive, real-time, in vivo monitoring of longitudinal shifts in tissue physiology in response to
developmental, hormonal, or environmental influences. The objective of the proposed research is to pursue a
multi-pronged platform with complementary Raman scattering approaches to study longitudinal changes in tissue
physiology in vivo as well as ex vivo and compare the results with conventional assays to validate our approach.
In addition, we will combine various forms of in vivo Raman imaging, including (i) dual wavelength, dual region
Raman (ii) polarization-sensitive Raman, and (iii) resonance Raman spectroscopy to quantitatively analyze
changes in tissue physiology. In response to a lack of detailed understanding of preterm birth and its tissue
biochemistry, our group has reported the utility of in vivo RS to detect and extract longitudinal biochemical
changes in the mouse as well as human cervix, a tissue that undergoes extensive remodeling over the course
of pregnancy. We established the ability of a conventional in vivo RS probe to identify significant spectral changes
in collagen, elastin, water, and blood. Moreover, we can correlate these Raman spectra with changes in
biomechanical properties of the mouse cervix, including stiffness and distensibility, as the cervical tissue
undergoes normal remodeling/maturation in preparation for labor. Building on our prior research, the present
proposal will focus multimodal ex vivo and in vivo Raman approaches to gain in-depth, quantitative information
during physiologic cervical maturation and in mouse models of premature remodeling. We hypothesize that
multimodal Raman approaches will enable detection of key biochemical changes, such as extracellular matrix
(ECM) organization, tissue hydration, lipid and protein influx and vascularity that will allow molecular and
structural phenotyping of the cervix as a prototype of physiologic tissue remodeling. Results will be correlated
with spatial information obtained via ex vivo imaging and biomechanical testing. Our Aims are to: 1) Implement
multimodal non-linear imaging to characterize changes in structural proteins in the mouse cervix, 2) Use dual
wavelength, dual region Raman Spectroscopy to track changes in tissue hydration and lipid dynamics, and 3)
Evaluate spatial and temporal changes in blood and vascularization in the mouse cervix over the course of
pregnancy. Ultimately, this project will integrate the results of these aims to provide a more complete picture of
the molecular and structural changes that can be used to understand normal as well as compromised
pregnancies. The resultant in-depth biomolecular profiles and spatial tissue maps obtained for normal term and
preterm pregnancies with the innovative Raman approaches will provide vital information about the mechanism
of premature cervical remodeling and for monitoring longitudinal changes in the structural and biochemical
properties of other tissues with RS.
项目总结
检查组织结构和生化特性的纵向变化的方法有限
在各种生理条件下,如怀孕。光和基于光的技术提供了潜在的
用于非侵入性、实时、活体监测组织生理学的纵向移位
发育、荷尔蒙或环境的影响。拟议研究的目标是进行一项
用互补拉曼散射方法研究组织纵向变化的多管齐下平台
并将结果与常规检测方法进行比较,以验证我们的方法。
此外,我们将结合各种形式的活体拉曼成像,包括(I)双波长、双区域
拉曼(II)偏振敏感拉曼和(III)共振拉曼光谱定量分析
组织生理学的变化。由于缺乏对早产及其组织的详细了解
生物化学,我们小组报道了体内RS检测和提取纵向生化的应用
小鼠和人类宫颈的变化,宫颈组织在整个过程中经历了广泛的重塑
怀孕的迹象。我们建立了传统体内RS探测器识别显著光谱变化的能力
在胶原蛋白、弹性蛋白、水和血液中。此外,我们可以将这些拉曼光谱与
小鼠宫颈的生物力学性质,包括僵硬和膨胀性,如宫颈组织
经历正常的重塑/成熟,为分娩做准备。在我们先前研究的基础上,目前
提案将重点关注多模式体外和体内拉曼方法,以获得深入的、定量的信息
在生理性宫颈成熟和小鼠早产重塑模型中。我们假设
多模拉曼方法将能够检测关键的生化变化,如细胞外基质
(ECM)组织,组织水化,脂肪和蛋白质流入和血管,将使分子和
宫颈结构表型作为生理性组织重塑的原型。结果将相互关联
通过体外成像和生物力学测试获得空间信息。我们的目标是:1)实施
多模式非线性成像用于表征小鼠宫颈结构蛋白的变化,2)使用DUAL
波长,双区域拉曼光谱,以跟踪组织水化和脂质动力学的变化,以及3)
评价小鼠子宫颈血流和血管形成的时空变化
怀孕了。最终,该项目将整合这些目标的结果,以提供更完整的图景
可以用来理解正常和受损的分子和结构变化
怀孕。由此得到的生物分子深度分布和空间组织图在正常和
利用创新的拉曼方法进行早孕将提供有关该机制的重要信息
早期宫颈重塑和监测结构和生化的纵向变化
RS的其他组织的特性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Anita Mahadevan-Jansen其他文献
Anita Mahadevan-Jansen的其他文献
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{{ truncateString('Anita Mahadevan-Jansen', 18)}}的其他基金
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双波长内窥镜拉曼探头治疗嗜酸性食管炎
- 批准号:
10653201 - 财政年份:2022
- 资助金额:
$ 66.79万 - 项目类别:
Complementary ex vivo multimodal optical imaging and in vivo Raman spectroscopy to understand tissue dynamics
补充离体多模态光学成像和体内拉曼光谱以了解组织动力学
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Detecting biochemical changes in the pregnant mouse cervix by Raman spectroscopy
拉曼光谱检测妊娠小鼠子宫颈的生化变化
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8925122 - 财政年份:2014
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Detecting biochemical changes in the pregnant mouse cervix by Raman spectroscopy
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8766404 - 财政年份:2014
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Development of breakthrough intra-operative detector and imaging system for parat
开发突破性的术中探测器和成像系统
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拉曼光谱-光学断层扫描相结合,可对皮肤进行全面诊断
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