B-CELL FUNCTION OF TNP-ABC PURIFIED FROM AGING MODEL

为了了解B细胞在老年人免疫系统中的功能， 动物，我们计划研究抗原诱导的生长和分化， 抗原特异性群体。 最初，B细胞向两种细胞的扩增 胸腺非依赖性（TI）和胸腺依赖性（TD）抗原将被 评估并与活化细胞的分化相关， 抗体形成细胞（AFC）。 这些实验将检验 的B细胞增殖，以检验这一假设， 细胞在受到攻击后不能有效地提供合适的后代， 抗原的 其次，TD抗原激活的B细胞对 非特异性巨噬细胞和T细胞生长和分化细胞因子将 被评价。 细胞因子对老年小鼠B细胞影响的降低可能 参与这些细胞的整体能力，以扩大后， 启动免疫反应。 第三，记忆B细胞的能力 从老年小鼠中扩增并为第二代提供足够的后代 将检查免疫应答。 这些实验的结果应该 有助于我们理解B细胞在免疫系统中的功能。 老化的免疫系统，独立于可能发生在 辅助细胞群

In order to understand B cell function within the immune system of old animals, we plan to study the antigen-induced growth and differentiation of antigen-specific populations. Initially, the expansion of B cells to both thymus-independent (TI) and thymus-dependent (TD) antigens will be evaluated and correlated to differentiation of the activated cells to antibody-forming cells (AFC). These experiments will examine the ability of B cells from aging mice to proliferate to test the hypothesis that such cells are inefficient in providing suitable progeny following challenge by antigen. Secondly, the responsiveness of TD antigen activated B cells to nonspecific macrophage and T cell growth and differentiation cytokines will be evaluated. Decreased cytokine influences on B cells from old mice could be involved in the overall inability of these cells to expand following the initiation of the immune response. Thirdly, the ability of memory B cells from old mice to expand and provide sufficient progeny for the secondary immune response will be examined. The results of these experiments should contribute significantly to our understanding of B cell function in the aging immune system, independent of defects which may occur in the accessory cell populations.