ROLE OF AMINES IN ALCOHOLIC BEVERAGES ON THE GI TRACT

某些酒精饮料刺激胃酸的机制 对分泌物的了解还不完全。关于药效的证据 乙醇刺激产酸与最近的研究相矛盾 表现出抑制作用的。相比之下，有一种相当统一的 同意未蒸馏的酒精饮料(即葡萄酒和啤酒) 胃酸分泌的有效刺激剂似乎是 归因于它们刺激胃泌素释放的能力。化学物质 葡萄酒和啤酒中负责触发分泌的成分(S) 然而，这种荷尔蒙还没有确定。在以前的研究中，我们的 实验室已经证明，挥发性胺，由 在这些酒精饮料中发现高浓度的发酵， 在体内和体外都是胃泌素释放的有效刺激剂。 因此，在这项建议中，我们计划调查 简单的脂肪胺以及芳香胺、酪胺 胃泌素分泌反应(胃泌素释放和胃酸分泌) 这些饮料在老鼠和狗身上的灌胃给药。胺 分析将通过荧光或定性方法进行 比色反应并用气相色谱进行定量。胃泌素 释放将通过放射免疫法测定，胃酸分泌 将在实验室动物身上用手术准备的胃进行研究 用自动滴定仪测量。因此，这项研究应该 洞察某些酒精饮料的作用机制 促进消化过程，以及这种GI行为是有害的还是 对机体有治疗作用。

The mechanism by which certain alcoholic beverages stimulate gastric acid secretion is not fully understood. The evidence concerning the efficacy of ethanol to stimulate acid output is conflicting with recent studies demonstrating an inhibitory action. In contrast there is a rather uniform agreement that non-distilled alcoholic beverages (i.e. wines and beers) are effective stimulants of gastric acid secretion which appears to be attributable to their ability to stimulate gastrin release. The chemical constituent(s) in wine and beer responsible for triggering the secretion of this hormone, however, have yet to be identified. In previous studies our laboratory has demonstrated that volatile amines, compounds generated by fermentation and found in high concentration in these alcoholic beverages, are potent stimulants of gastrin release both in vivo and in vitro. Accordingly in this proposal we plan to investigate the contribution of the simple aliphatic amines as well as the aromatic amine, tyramine, to the gastric secretory response (gastrin release and acid secretion) elicited by the intragastric administration of these beverages in rats and dogs. Amine analysis will be performed both qualitatively by fluorescence or colorimetric reactions and quantitatively by gas chromatography. Gastrin release will be determined by radioimmunoassay, and gastric acid secretion will be studied in laboratory animal with surgically prepared gastric pouches and measured with an autotitrator. This study, therefore, should provide insight into mechanism by which certain alcoholic beverages facilitate the digestive process, and whether this GI action is harmful or therapeutic to the organism.