ROLE OF AMINES IN ALCOHOLIC BEVERAGES ON THE GI TRACT

某些酒精饮料刺激胃酸的机制 分泌还不完全清楚。 关于有效性的证据 乙醇刺激酸输出与最近的研究相矛盾 表现出抑制作用。 相比之下， 非蒸馏酒精饮料（即葡萄酒和啤酒） 胃酸分泌的有效刺激物， 归因于它们刺激胃泌素释放的能力。 化学 葡萄酒和啤酒中的一种成分， 然而，这种激素还有待鉴定。 在以前的研究中， 实验室已经证明，挥发性胺，化合物产生的 发酵和发现在这些酒精饮料中的高浓度， 是体内和体外胃泌素释放的强效刺激剂。 因此，在本提案中，我们计划调查 简单的脂肪胺以及芳香胺，酪胺， 胃分泌反应（胃泌素释放和酸分泌）由 这些饮料在大鼠和狗的胃内给药。 胺 分析将通过荧光定性或 比色反应和气相色谱定量。 胃泌素 释放将通过放射免疫测定和胃酸分泌来确定 将在实验室动物中进行研究， 袋中，并用自动滴定仪测量。 因此，这项研究应 让我们深入了解某些酒精饮料 促进消化过程，以及这种GI作用是否有害或 对机体有治疗作用。

The mechanism by which certain alcoholic beverages stimulate gastric acid secretion is not fully understood. The evidence concerning the efficacy of ethanol to stimulate acid output is conflicting with recent studies demonstrating an inhibitory action. In contrast there is a rather uniform agreement that non-distilled alcoholic beverages (i.e. wines and beers) are effective stimulants of gastric acid secretion which appears to be attributable to their ability to stimulate gastrin release. The chemical constituent(s) in wine and beer responsible for triggering the secretion of this hormone, however, have yet to be identified. In previous studies our laboratory has demonstrated that volatile amines, compounds generated by fermentation and found in high concentration in these alcoholic beverages, are potent stimulants of gastrin release both in vivo and in vitro. Accordingly in this proposal we plan to investigate the contribution of the simple aliphatic amines as well as the aromatic amine, tyramine, to the gastric secretory response (gastrin release and acid secretion) elicited by the intragastric administration of these beverages in rats and dogs. Amine analysis will be performed both qualitatively by fluorescence or colorimetric reactions and quantitatively by gas chromatography. Gastrin release will be determined by radioimmunoassay, and gastric acid secretion will be studied in laboratory animal with surgically prepared gastric pouches and measured with an autotitrator. This study, therefore, should provide insight into mechanism by which certain alcoholic beverages facilitate the digestive process, and whether this GI action is harmful or therapeutic to the organism.