CHANGES IN SKELETAL MUSCLE SATELLITE CELLS DURING AGING

衰老过程中骨骼肌卫星细胞的变化

• 批准号: 3114715
• 负责人: RONALD E ALLEN
• 金额: $ 8.36万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-05-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3114715
• 关键词: aging; animal age group; atrophy; cell cycle; degenerative motor system disease; growth factor receptors; hormone receptor; laboratory mouse; laboratory rat; molecular pathology; myoblasts; radiotracer; receptor mediated endocytosis; striated muscles; tissue /cell culture

Senile muscle atrophy represents one of the major problems associated with aging and is characterized by loss of strength and muscle mass. These changes restrict the mobility and independence of a growing segment of our society. This project will probe the cellular and molecular changes that occur during muscle aging. In particular, the function of skeletal muscle satellite cells will be evaluated. Satellite cells are the myogenic precursor cells that persist in muscle throughout life and function by adding new nuclei to existing muscle fibers during muscle hypertrophy and by regenerating new fibers following injury. Their ability to proliferate and differentiate in response to three important regulatory protein growth factors, or hormones, is being studied. These hormones are the two insulin-like growth factors (somatomedins) and fibroblast growth factor. In vitro techniques have been devised for monitoring the effects of growth factors on the proliferation and differentiation of cultured satellite cells, and these techniques will be employed to study the normal control of satellite cell activity. In addition, age-related changes in the ability of satellite cells to respond to these growth factors and changes in their growth factor receptor characteristics during aging will be examined. This research program is designed to provide fundamental insights into the cellular and molecular mechanisms of muscle aging with the ultimate goal of contributing toward the alleviation of problems associated with senile muscle atrophy.

老年性肌肉萎缩是与以下疾病相关的主要问题之一 衰老，以失去力量和肌肉质量为特征。这些 变化限制了我们日益增长的部分人的流动性和独立性 社会。 这个项目将探索细胞和分子发生的变化 在肌肉老化过程中。特别是，骨骼肌的功能 将对卫星电池进行评估。卫星细胞是肌源性的 前体细胞在肌肉中终生存在，并通过 在肌肉肥大期间向现有的肌肉纤维添加新的细胞核 通过在受伤后再生新的纤维。它们的增殖能力 并根据三种重要的调节蛋白的生长情况进行分化 因素，或荷尔蒙，正在研究中。这些荷尔蒙是两种 胰岛素样生长因子(生长抑素)和成纤维细胞生长因子。 已经设计了体外技术来监测生长的影响 影响培养卫星增殖分化的因素 这些技术将被用来研究细胞的正常控制。 卫星细胞活动。此外，与年龄相关的能力变化 卫星细胞对这些生长因子的反应以及它们的变化 将检查老化过程中的生长因子受体特性。这 研究计划旨在提供对 肌肉衰老的细胞和分子机制的最终目标是 为缓解与老年人有关的问题作出贡献 肌肉萎缩。