TOXICOLOGY OF PULMONARY OXIDANT INJURY IN AGING
TOXICOLOGY OF PULMONARY OXIDANT INJURY IN AGING
批准号:
3119128
负责人:
MARK R MONTGOMERY
金额:
$7.26万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-08-01 至 1993-07-31
关键词:
NAD(H) phosphate aging animal old age bioenergetics catalase cell growth regulation environmental toxicology enzyme linked immunosorbent assay enzyme mechanism histology laboratory rat lung disorder mature animal membrane permeability mitochondria nicotinamide adenine dinucleotide nucleoside monophosphate oxidative phosphorylation ozone physical chemical interaction respiratory epithelium respiratory gas transport respiratory toxin statistics /biometry superoxide dismutase tocopherols
中文摘要
肺力学在衰老过程中的进行性恶化
被反复记录下来。 衰老的生物化学方面
肺受到的关注要少得多,特别是,
肺生物能量学根本没有被研究过。
老化肺中的生物能量学对于
细胞活力和寿命,因此,维持
适当的器官功能和抵抗疾病和伤害。 这
该项目将检验老年肺具有
生物能代谢能力降低,
成人器官 具体来说,肺上皮将是
调查的重点。 这个肺表面是气体
交易所,因此,是许多类型的
肺损伤 该假说预测上皮细胞
老年人的肺对损伤的反应会降低。 到
为了研究这一点,一种可重复氧化性肺损伤模型
使用急性暴露(8小时)对三种
臭氧浓度。 在这个模型中,特定的和独立的
上皮损伤、细胞增殖和恢复的阶段
已被定义。 这些阶段中的每一个都可以在
损伤分级水平定义为轻度、中度和重度,
上皮损伤的程度。 为了全面评估
这种典型的氧化损伤的生物能量后果,
线粒体和细胞质的生物能代谢将是
研究了 此外,这种损伤的细胞后果,
在成年和老年动物中,将根据脂质和
蛋白质损伤和防御反应。 所有生化研究
将与两者出现的形态测量变化相关
在整个器官和分离的II型肺上皮中。
这些综合研究将提供关于
衰老对正常、健康人肺生物能量学的影响
哺乳动物和在肺损伤的情况下,从化学品
这是许多常见肺部毒素的典型特征
英文摘要
Progressive deterioration of pulmonary mechanics during aging
has been documented repeatedly. Biochemical aspects of aging in
the lung have received much less attention, and in particular,
pulmonary bioenergetics have not been investigated at all.
Bioenergetics in the aging lung are of immense importance to
cellular viability and longevity, and hence, to maintenance of
proper organ function and resistance to disease and injury. This
project will examine the hypothesis that the aged lung has a
decreased capacity for bioenergetic metabolism compared to the
adult organ. Specifically, the pulmonary epithelium will be the
focus of investigation. This lung surface is the site of gas
exchange and as such, is the initial site for many types of
pulmonary injury. The hypothesis predicts that the epithelial cells
in aged lungs will respond less efficiently to injury. To
investigate this, a reproducible model of oxidant lung injury has
been developed using acute exposure (8 hours) to three
concentrations of ozone. In this model, specific and separate
phases of epithelial injury, cellular proliferation and recovery
have been defined. Each of these phases may be ovserved during
graded levels of injury defined as mild, moderate and severe by
the extent of epithelial damage. To fully evaluate the
bioenergetic consequences of this typical oxidant injury, both
mitochondrial and cytoplasmic bioenergetic metabolism will be
investigated. Also, the cellular consequences of this injury, both
in adult and aged animals, will be quantitated in terms of lipid and
protein damage and defensive responses. All biochemical studies
will be correlated with the morphoetric alterations appearing both
in the whole organ and in isolated Type II pulmonary epithelium.
These combined studies will provide the first information on the
effects of aging on pulmonary bioenergetics in the noral, healthy
mammal and during the situation of lung injury from a chemical
that is typical of numerous common pulmonary toxins.
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TOXICOLOGY OF PULMONARY OXIDANT INJURY IN AGING
-
批准号:3119131
-
项目类别:
-
资助金额:$7.41万
-
财政年份:1988
-
负责人:MARK R MONTGOMERY
-
依托单位:
TOXICOLOGY OF PULMONARY OXIDANT INJURY IN AGING
-
批准号:3119132
-
项目类别:
-
资助金额:$7.33万
-
财政年份:1988
-
负责人:MARK R MONTGOMERY
-
依托单位:
TOXICOLOGY OF PULMONARY OXIDANT INJURY IN AGING
-
批准号:3119130
-
项目类别:
-
资助金额:$7.36万
-
财政年份:1988
-
负责人:MARK R MONTGOMERY
-
依托单位:
TOXICOLOGY OF PULMONARY OXIDANT INJURY IN AGING
-
批准号:3119133
-
项目类别:
-
资助金额:$7.52万
-
财政年份:1988
-
负责人:MARK R MONTGOMERY
-
依托单位:
FEMALE EDUCATION AND AGE AT MARRIAGE
-
批准号:3321590
-
项目类别:
-
资助金额:$8.67万
-
财政年份:1986
-
负责人:MARK R MONTGOMERY
-
依托单位:
XENOBIOTIC METABOLISM AND PARAQUAT TOXICITY
-
批准号:3250542
-
项目类别:
-
资助金额:$7.47万
-
财政年份:1985
-
负责人:MARK R MONTGOMERY
-
依托单位:
XENOBIOTIC METABOLISM AND PARAQUAT TOXICITY
-
批准号:3250540
-
项目类别:
-
资助金额:$7.82万
-
财政年份:1985
-
负责人:MARK R MONTGOMERY
-
依托单位:
XENOBIOTIC METABOLISM AND PARAQUAT TOXICITY
-
批准号:3250543
-
项目类别:
-
资助金额:$7.08万
-
财政年份:1985
-
负责人:MARK R MONTGOMERY
-
依托单位:
海外基金