MECHANISMS OF NONSTERILIZING IMMUNITY IN MALARIA

小鼠对急性查鲍迪阿达米疟疾的抗药性是介导性的 通过非抗体机制的免疫。传导性细胞移植研究 证明T细胞是诱导和表达所必需的 这种豁免权。拟议研究的目的是 确定脾T细胞的哪些Lyt亚群参与其中。LYT子集 选择性杀伤胸腺小鼠脾细胞的实验研究 补体存在或平移时的抗Lyt单抗 大鼠抗鼠Lyt包被的脾细胞在包被抗鼠抗体的培养板上的应用 免疫球蛋白将注射到可组织复制的裸鼠体内，然后 与P.chadaudi Adami一起挑战。由此产生的寄生虫病将是 为了看看哪个Lyt子集(S)压制了 感染。此外，从小鼠的脾中制备的脾细胞的LYT亚群 沙包氏疟原虫Adami免疫B细胞缺陷小鼠及其免疫学 完好的小鼠将被测试它们的过继转移能力 对辐照同系物的P-Chabaudi Adami感染的免疫力 收件人。为明确P。 Chabaudi Adami T细胞克隆及脾T细胞杂交瘤细胞 将测试免疫小鼠的细胞保护裸鼠的能力 对抗挑战感染。同样，来自保护性的可溶性因子 将对细胞系进行表征，并测试它们的中介能力 对裸鼠的保护。 将调查另外两个参数，以确定 它们是否可以与Lyt组分实现的保护相关 T细胞克隆和T细胞杂交体。这些是脾巨噬细胞的激活 通过化学发光和脾网状结构的激活来确定 细胞。最后，查鲍迪巴氏杆菌Adami抗原将被分离并 以激活保护细胞的能力为特征的 并通过免疫诱导对小鼠的保护。 这种方法将尝试将保护与细胞 功能、因子产生和效应细胞激活，可以提供 事件的连贯生理图景，必须为 表达抗体非依赖性免疫。

Resistance to acute Plasmodium chabaudi adami malaria in mice is mediated by non-antibody mechanisms of immunity. Adoptive cell transfer studies demonstrate that T cells are required for both the induction and expression of this form of immunity. The purpose of the proposed study is to determine which Lyt subsets of splenic T cells are involved. Lyt subsets of spleen cells from euthymic mice prepared by selective killing with anti-Lyt monoclonal antibody in the presence of complement or by panning rat anti-mouse Lyt coated spleen cells on petri plates coated with anti-rat Ig will be injected into histocopatible athymic nude mice prior to challenge with P. chadaudi adami. The resulting parasitemias will be plotted against time in order to see which Lyt subset(s) suppress infection. Also, Lyt subsets of spleen cells prepared from the spleens of P. chabaudi adami immune B-cell deficient mice as well as immunologically intact mice will be tested for their ability to adoptively transfer immunity to P chabaudi adami infection to irradiated homologous recipients. In order to define mechanisms of nonantibody immunity to P. chabaudi adami T-cell clones and T-cell hybrids constructed with spleen cells of immune mice will be tested for their capacity to protect nude mice against challenge infection. Similarly, soluble factors from protective cell lines will be characterized and tested for their ability to mediate protection in nude mice. Two additional parameters will be investigated in order to determine whether they can be correlated with protection achieved with Lyt fraction T-cell clones and T-cell hybrids. These are splenic macrophage activation as determined by chemiluminescence and the activation of splenic reticular cells. Finally, P. chabaudi adami antigens will be fractionated and characterized in terms of their ability to activate protecting cell populations as well as to induce protection in mice by immunization. This approach, which will attempt to correlate protection with cell function, factor-production, and effector cell activation, may provide a coherent physiologic picture of events which must transpire for the expression of antibody-independent immunity.