IMMUNOLOGY OF CUTANEOUS BASOPHIL HYPERSENSITIVITY

通过对皮肤嗜碱性粒细胞超敏反应(CBH)的研究，现已明确 嗜碱性粒细胞和肥大细胞，它们所含的介质(如组胺 和5-羟色胺)及其相关的过敏性抗体(IgE和 免疫球蛋白1)，参与延迟性过程反应。这些发现意味着 在过敏性疾病和Astha中起重要作用的免疫成分也 在对微生物和寄生虫的免疫抵抗力以及在 肿瘤和组织移植的排斥反应。这份研究提案提交了一份 继续调查的全面方案 豚鼠CBH反应中的抗体。我们将通过以下方式来研究这一机制 这些抗体通过确定循环中的 嗜碱性细胞和到达CBH反应的嗜碱性细胞被敏化 转移CBH的抗体。我们还将确定这些 抗体是IgG1的一个特殊亚类，无论是淋巴细胞还是 巨噬细胞参与了抗体介导CBH的能力。 最后，将进行研究以确定这些抗体是否 也参与由胸腺来源的CBH反应 淋巴细胞(T细胞)。有关规定的研究亦会继续进行。 使T细胞激活肥大细胞释放血管活性胺 5-羟色胺与小鼠迟发型超敏反应(DTH)5-羟色胺 在迟发型变态反应中肥大细胞的转运，以及体外的研究。一根桅杆 细胞衍生的5-羟色胺蛋白可能在运输过程中起重要作用 5-羟色胺将被描述为。最后，我们将确定其机制 T细胞可通过决定是否 非特异性5-羟色胺释放淋巴因子，或特异性IGF样因子 都牵涉其中。

Through study of cutaneous basophil hypersensitivity (CBH) it is now clear that basophils and mast cells, their contained mediators (such as histamine and serotonin), and their associated anaphylactic antibodies (IgE and IgG1), are involved in delayed-time course reactions. These findings mean that immune components improtant in allergic disease and astha are also potentially important in immune resistance to microbes and parasites and in rejection of tumors and tissue grafts. This research proposal submits a comprehensive program for the continued investigation of the role of antibodies in CBH responses of guinea pigs. We will study the mechanism by which these antibodies mediate CBH by determining whether circulating basophils, and basophils arriving at CBH reactions are sensitized with antibodies that transfer CBH. We will also determine whether these antibodies are a special subclass of IgG1 and whether lymphocytes or macrophages are involved in the ability of antibodies to mediate CBH. Finally, studies will be performed to determine whether these antibodies are also involved in CBH reactions that are transferred by thymic-derived lymphocytes (T cells). Studies will also be continued on the requirement for T cells to activate mast cells to release the vasoactive amine serotonin in murine delayed-type hypersensitivity (DTH). Serotonin transport from mast cells in DTH, and in vitro will be studied. A mast cell-derived serotonin protein that may be important in transport of serotonin will be characterized. Finally, we will determine the mechanism by which T cells may activate mast cells in DTH by determining whether non-specific serotonin releasing lymphokines, or specific IgF-like factors are involved.