CELL BIOLOGY OF THE IMMUNE RESPONSE TO BACTERIA

该项目将继续评估宿主参与的免疫活性细胞 抵抗细胞内细菌的感染。单核细胞增多性李斯特菌 以小鼠体内感染为模型系统。我们正在指挥 试验以确定为什么注射李斯特氏菌的部分或 感染活体李斯特菌前的硫乙醇酸盐培养基会降低 老鼠抵抗感染的能力。实验证实了这一发现 目前正在进行体外实验和机制评估。另一种方法是 李斯特菌特异性T细胞的选择性增殖，其功能将是 已评估。李斯特菌提取物的生物学效应研究包括其 作为有丝分裂原和佐剂，作为抗原和激活剂的能力 巨噬细胞。巨噬细胞的激活以杀伤肿瘤细胞为标准 我们用来测量巨噬细胞的活性。

This project will continue to evaluate immunocompetent cells involved in host resistance to infection by intracellular bacteria. Listeria monocytogenes infection in the mouse is used as the model system. We are conducting experiments to determine why injection of either Listeria fractions or thioglycollate medium prior to infection with live Listeria decreases the mouse's ability to resist infection. Experiments to confirm this finding in vitro and to evaluate the mechanism are in progress. Another approach is selective propagation of Listeria specific T cells, whose function will be evaluated. Studies on biologic effects with Listeria fractions include their ability to function as mitogens and adjuvants, as antigens, and as activators of macrophages. Activation of macrophages to kill tumor cells is the criterion by which we measure macrophage activation.