CELL BIOLOGY OF THE IMMUNE RESPONSE TO BACTERIA

对细菌免疫反应的细胞生物学

• 批准号: 3124926
• 负责人: PRISCILLA A CAMPBELL
• 金额: $ 9.81万
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-08-01 至 1986-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124926
• 关键词: B lymphocyte; Listeria; T lymphocyte; bacterial vaccines; bactericidal immunity; cell biology; cellular immunity; hematopoietic tissue; humoral immunity; macrophage; microorganism immunology; phagocytosis

This project will continue to evaluate immunocompetent cells involved in host resistance to infection by intracellular bacteria. Listeria monocytogenes infection in the mouse is used as the model system. We are conducting experiments to determine why injection of either Listeria fractions or thioglycollate medium prior to infection with live Listeria decreases the mouse's ability to resist infection. Experiments to confirm this finding in vitro and to evaluate the mechanism are in progress. Another approach is selective propagation of Listeria specific T cells, whose function will be evaluated. Studies on biologic effects with Listeria fractions include their ability to function as mitogens and adjuvants, as antigens, and as activators of macrophages. Activation of macrophages to kill tumor cells is the criterion by which we measure macrophage activation.

本项目将继续评估参与宿主免疫活性细胞 对细胞内细菌感染的抵抗力。 单核细胞增生李斯特菌 使用小鼠中的感染作为模型系统。 我们正在进行 实验来确定为什么注射李斯特菌部分或 在感染活李斯特菌之前使用硫乙醇酸盐培养基， 小鼠抵抗感染的能力。 实验证实了这一发现， 体外研究及机制探讨正在进行中。 另一种方法是 李斯特菌特异性T细胞的选择性增殖，其功能将是 评估。 李斯特菌部分的生物学效应的研究包括其 作为有丝分裂原和佐剂，作为抗原和作为 巨噬细胞 巨噬细胞的活化以杀死肿瘤细胞是标准， 我们测量巨噬细胞的活性。