FUNCTION OF THE KINETOPLAST IN THE HEMOFLAGELLATES

这个项目的总体目标是解剖线粒体的过程， 在这种低等真核寄生细胞中， 在线粒体基因组和核基因组方面。 此外，我们想了解不寻常的分子 这种线粒体DNA在结构和功能上的分化 道理啊 具体目标如下：1.完成整个序列 L.大圆塔伦托拉 2.所有的3'和5'末端的鉴定 成绩单 3.主要成绩单的鉴定。 4.分离与 tRNA的测序。 5.特异性大环基因的转录 不同的生理条件。 6.大环基因的转录 在T.布鲁塞。 7.线粒体RNA聚合酶的分离。 大环基因启动子的鉴定。 8.比较上颌圆 不同物种的基因结构。 9. 9和12 s基因的测序 几个物种。 10.细胞色素氧化酶或FOF 1 ATP酶的分离 距离L. tarentolae 11.细胞色素氧化酶核基因的克隆 FOF 1 ATPase亚基4-7。 12.克隆细胞核基因 线粒体蛋白 13.发展调节分析 在T.布鲁塞。 14.分析可能的 小环转录

The overall goal of this project is to dissect the process of mitochondrial biogenesis that occurs in this lower eukaryotic parasitic cell in molecular terms, both in terms of the mitochondrial genome and the nuclear genome. In addition we would like to understand the unusual molecular differentiation of this mitochondrial DNA in a structural and functional sense. Specific aims are as follows: 1. Completion of entire sequence of L. tarentolae maxicircle. 2. Identification of 3' and 5' ends of all transcripts. 3. Identification of primary transcripts. 4. Isolation and sequencing of tRNAs. 5. Transcription of specific maxicircle genes under different physiological conditions. 6. Transcription of maxicircle genes in life cycle of T. brucei. 7. Isolation of mitochondrial RNA polymerase. Identification of promoters of maxicircle genes. 8. Comparative maxicircle gene organization in different species. 9. Sequencing of 9 and 12s genes from several species. 10. Isolation of cytochrome oxidase or FOF1 ATPase from L. tarentolae. 11. Cloning of nuclear genes for cytochrome oxidase subunits 4-7 of FOF1 ATPase subunits. 12. Cloning nuclear genes for mitochondrial proteins. 13. Analysis of developmentally regulated transcription of the BhR sequence in T. brucei. 14. Analysis of possible minicircle transcription.