IMMUNOLOGIC STUDY OF HUMAN HLA AND MOUSE H-2 ANTIGENS

人 HLA 和小鼠 H-2 抗原的免疫学研究

• 批准号: 3124465
• 负责人: D. BERNARD AMOS
• 金额: $ 35.96万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1974
• 资助国家: 美国
• 起止时间: 1974-10-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124465
• 关键词: B lymphocyte; Coxsackievirus; HY antigen; affinity chromatography; antibody dependent killer cell; antibody formation; antibody specificity; antigen antibody reaction; antileukocyte isoantibody; cell sorting; clone cells; electrofocusing; fluorescence; glycoproteins; helper T lymphocyte; histocompatibility antigens; human tissue; hybridomas; immune tolerance /unresponsiveness; immunochemistry; immunodeficiency; isoantibody; laboratory mouse; laboratory rabbit; laboratory rat; lymphoblast; macrophage; major histocompatibility complex; measles; membrane proteins; microorganism classification; monoclonal antibody; mutagens; plaque assay

The objective is to gain precise knowledge of certain cell surface markers especially those of lymphoid cells, and how each can serve as a differentiation signal or trigger the activities of another cell. Most of the effort is directed at components of the major histocompatibility complex of man, HLA, which includes two distinct types of antigen, Class I and Class II. Only 3 Class I molecules have been identified although over 30 genes exist. One of our aims is to determine if other genes in this cluster (which we call a congener) are operational in cells of certain tissue such as thymus or vascular endothelium or if some develop only at specific stages of differentiation. Another aim is to produce monoclonal antibodies to the Class I and Class II antigens. The Class II congener includes at least 9 functioning genes but only 3 or 4 of the products, transmembranous glycoprotein heterodimers can clearly be distinguished. Having produced monoclonal antibodies to at least some of the individual Class II glycoproteins and to the 2 or more epitopes probably carried by each, we wish to know if each molecule has separate function and we ask the complementary question "do the different epitopes of the same molecule even have different function". We believe that the answers to these questions will help us understand why some people form antibodies after transfusion and some do not, why some reject kidneys and others do not, and how we might, through such detailed analyses understand and control immune responses in health and in diseases such as AIDS and autoimmunity. Since we can learn about cellular function by an analysis of mutant cells, we propose to screen for somatic mutants. We shall also look for new markers of B lymphocytes and plasma cells. In a separate series of experiments in mice we plan to study the way that the MHC molecules are transported to the surface of the phagocytic cell since this may help us understand the way that highly complex molecules function as antigens.

目的是获得某些细胞表面标记物的精确知识

项目成果

Trypanosoma brucei sspp.: cleavage of variant specific and common glycoproteins during exposure of live cells to trypsin.

布氏锥虫亚种：活细胞暴露于胰蛋白酶期间，变异特异性和常见糖蛋白的裂解。

• DOI: 10.1016/0014-4894(88)90093-8
• 发表时间: 1988
• 期刊: Experimental parasitology
• 影响因子: 2.1
• 作者: Frommel,TO;Seyfang,A;Balber,AE
• 通讯作者: Balber,AE

Binding of bacillus Calmette-Guérin-activated macrophages to tumor targets. Selective inhibition by membrane preparations from homologous and heterologous neoplastic cells.

• DOI: 10.1084/jem.154.1.77
• 发表时间: 1981-07-01
• 期刊: The Journal of experimental medicine
• 作者: Marino PA;Whisnant CC;Adams DO
• 通讯作者: Adams DO

B cell sensitivity to natural killing: correlation with target cell stage of differentiation and state of activation.

B 细胞对自然杀伤的敏感性：与靶细胞分化阶段和激活状态的相关性。

• 发表时间: 1986
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Storkus,WJ;Dawson,JR
• 通讯作者: Dawson,JR

A mutation in a non-MHC murine cell surface antigen detectable by cytotoxic T lymphocytes.

细胞毒性 T 淋巴细胞可检测到的非 MHC 鼠细胞表面抗原的突变。

• 发表时间: 1986
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Rinchik,EM;Amos,DB
• 通讯作者: Amos,DB

The pellicle and the membrane of the flagellum, flagellar adhesion zone, and flagellar pocket: functionally discrete surface domains of the bloodstream form of African trypanosomes.

鞭毛的表膜和膜、鞭毛粘附区和鞭毛袋：非洲锥虫血流形式的功能离散表面区域。

• 发表时间: 1990
• 期刊: Critical reviews in immunology
• 影响因子: 1.3
• 作者: Balber,AE