REPLICATIVE MECHANISMS OF THE NEGATIVE STRAND VIRUSES

急性病毒感染通常与细胞杀伤有关， 主要的细胞功能可能会被抑制或破坏， 病毒复制的过程。 阴性急性病毒 链RNA组包括麻疹、腮腺炎、狂犬病、呼吸道 合胞病毒和用于分子研究的模型原型， 水泡性口炎病毒（VSV）。 本提案的目标 是了解病毒与宿主相互作用的分子本质 导致与VSV感染相关的细胞病理学。 这些实验将集中在核酸-蛋白质相互作用 被认为影响宿主细胞大分子事件， 转录和RNA加工。 我们将使用抗体 沉淀、重组DNA方法、细胞培养技术和 体外生化分析。 我们的目标是定义 在分子水平上，这些RNA-蛋白质接触改变了细胞的 功能，以了解机制：1）细胞杀伤， VSV：2）从急性转为持续感染：3）细胞 RNA转录和加工。 因此，我们对VSV感兴趣 作为一个具有独特复制特性的生物实体， 病理学和作为探针研究哺乳动物细胞的功能。

Acute viral infections are usually associated with cell killing in that major cellular functions may be inhibited or destroyed in the Process of replication of the virus. Acute viruses of the negative strand RNA group include measles, mumps, rabies, respiratory syncytial virus and the model prototype for molecular studies, vesicular stomatitis virus (VSV). The objectives of this proposal are to understand the molecular nature of virus-host interactions that lead to the cytopathology associated with infection by VSV. These experiments will focus on nucleic acid-protein interactions that are thought to affect host cell macromolecular events such as transcription and RNA processing. We will use antibody precipitation, recombinant DNA methods, cell culture techniques and in vitro biochemical analysis. Our goals are to define at the molecular level those RNA-protein contacts that alter cellular functions in order to understand mechanisms of: 1) cell killing by VSV: 2) conversion from acute to persistent infection: 3) cellular RNA transcription and processing. Thus, we are interested in VSV as a biological entity with unique properties of replication and pathology and as a probe to study the functions of mammalian cells.