STRUCTURE AND FUNCTION OF THE GENOMES OF SMALL VIRUSES

这项工作的目的是修改mRNA的基因组， 病毒BMV（雀麦花叶病毒）、Q β噬菌体和 SBMV（南方菜豆花叶病毒）及其近缘种， 研究病毒基因组复制的基本过程， 蛋白质合成、病毒体组装及其相互关系。 进行这些修改的直接目标是 改变病毒与宿主的相互作用， 用于引入新的或替代的病毒功能的载体。 将构建病毒cDNA及其RNA 转录本是感染过程中的中间体， 使用强大的重组DNA化学技术。 将引入修饰以进一步鉴定蛋白质 编码在病毒基因组中，并描述其作用机制 并鉴定和表征基因组调控位点。

The purpose of this work is to modify the genomes of the mRNA viruses BMV (brome mosaic virus), Q beta bacteriophage and SBMV (southern bean mosaic virus) and their relatives in order to study the fundamental processes of viral genome replication, viral protein synthesis, virion assembly and their interrelationships. The immediate goals in making these modifications are both to alter virus-host interactions and to enable the construction of vectors for the introduction of new or alternative viral functions. Constructions will be made in which viral cDNAs and their RNA transcripts are intermediates in the infectious process in order to use the powerful techniques of recombinant DNA chemistry. Modifications will be introduced to further identify the proteins encoded in viral genomes and delineate their mechanism of action and to identify and characterize genomic regulatory sites.