DEVELOPMENTAL BIOLOGY OF STREPTOMYCES

链霉菌的发育生物学

• 批准号: 3125588
• 负责人: JERALD C ENSIGN
• 金额: $ 9.94万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-04-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3125588
• 关键词: Streptomyces; enzyme mechanism; hydrolase; laboratory rabbit; microorganism growth; microorganism metabolism; mutant; protein biosynthesis; protein structure; sporogenesis; trehalose

Streptomyces are the primary source of medically importnt antibiotics. This research project is designed at understanding the molecular and physiological processes involved in sporulation and spore germination of Streptomyces viridochromogenes and S. griseus. Germinating spores excrete and degrade intracellular reserves of trehalose and a glutamic acid-rich protein. Experiments are designed to determine the nature of the reserves and how the enzymes degrading them are activated during initiation of germination. Trehalose comprises 25-30% of the dry weight of spores. The idea that trehalose is involved in desiccation resistance and dormancy of spores is being studied. Non-sporulating streptomycin non-producing mutants of S. griseus acquire both properties when supplied a butyrolactone compound, A-factor, produced by the parent organisms. A-factor induces formation of NAD-glycohydrolase in the mutants. We will test the hypothesis that the glycohydrolase modifies sporulation-specific proteins by an ADP-ribosylatin reaction. ADP-ribosylation of proteins is a major mechanism of post-translational modificaton in eucaryotes. This work would be the fist report of this reaction as part of the normal growth and development of bacteria. There is a possibility that the modification involves expression of antibiotic synthesis as well as sporulation. We intend to study the glycohydrolase, clone the gene for its production in order to study its control, and to identify the proteins modified by the ADP-ribosylation reaction.

链霉菌是医学上重要抗生素的主要来源。 这项研究项目旨在了解分子和 参与孢子形成和孢子萌发的生理过程 绿色产色链霉菌（Streptomyces viridochromogenes）和S.灰色的 萌发孢子分泌 并降解细胞内海藻糖和富含谷氨酸的 蛋白 实验的目的是确定储备的性质 以及降解它们的酶如何在启动过程中被激活， 萌发 海藻糖占孢子干重的25-30%。 的 海藻糖参与植物抗脱水和休眠的观点 正在研究孢子。 不产链霉素 S.当提供丁内酯时，灰蝶会获得这两种特性 由母体生物产生的化合物A因子。 A因子诱导 突变体中NAD-糖水解酶的形成。 我们将测试 糖水解酶修饰孢子形成特异性蛋白质的假说 ADP-核糖基化反应。 蛋白质的ADP-核糖基化是一个主要的 真核生物中翻译后修饰的机制。 这项工作将 作为正常增长的一部分， 细菌的发展。 有一种可能性是， 涉及抗生素合成以及孢子形成的表达。 我们 打算研究糖水解酶，克隆其生产基因， 为了研究它的控制，并确定蛋白质修饰的 ADP-核糖基化反应。