MOSQUITO TOXIN PRODUCED BY XENORHABDUS SCAPTERISCII

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 斯氏杆菌是从昆虫病原线虫中分离到的一种伽马变形杆菌。该细菌被发现产生并分泌一种1350D分子，该分子对埃及伊蚊幼虫在皮克水平上是口服致死的。该毒素对其他昆虫或动物无毒，经生长液丙酮沉淀、离子交换、反相色谱分离纯化而成。该毒素是在以甘油为碳源和能源、以硫酸铵为氮源的特定培养基中产生的。该毒素将从含有C13甘油和N15铵的介质中制备，用于核磁共振分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Xenorhabdus scapteriscii is a gamma proteobacter isolated from an entomopathogenic nematode. The bacterium was found to produce and secrete a 1350D molecule that is orally lethal to Aedes aegypti mosquito larvae in picogram levels. It is not toxic to other insects or animals, The toxin is purified by acetone precipitation of growth liquor followed by ion exchange and reverse phase chromatography. The toxin is produced in defined media containing glycerol as carbon and energy source and ammonium sulfate as N source. The toxin will be prepared from media containing C13 glycerol and N15 ammonium for NMR analyses.