MOSQUITO TOXIN PRODUCED BY XENORHABDUS SCAPTERISCII

镰状致病杆菌产生的蚊子毒素

• 批准号: 8168950
• 负责人: JERALD C ENSIGN
• 金额: $ 0.11万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168950
• 关键词: Acetone; Aedes; Ammonium; Ammonium Sulfate; Animals; Bacteria; Carbon; Computer Retrieval of Information on Scientific Projects Database; Culicidae; Energy-Generating Resources; Funding; Glycerol; Grant; Growth; Insecta; Institution; Ion Exchange; Larva; Nematoda; Precipitation; Research; Research Personnel; Resources; Source; Toxin; United States National Institutes of Health; Xenorhabdus; distilled alcoholic beverage; reversed phase chromatography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Xenorhabdus scapteriscii is a gamma proteobacter isolated from an entomopathogenic nematode. The bacterium was found to produce and secrete a 1350D molecule that is orally lethal to Aedes aegypti mosquito larvae in picogram levels. It is not toxic to other insects or animals, The toxin is purified by acetone precipitation of growth liquor followed by ion exchange and reverse phase chromatography. The toxin is produced in defined media containing glycerol as carbon and energy source and ammonium sulfate as N source. The toxin will be prepared from media containing C13 glycerol and N15 ammonium for NMR analyses.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 斯氏黄杆菌是从昆虫病原线虫中分离的γ蛋白酶。 发现该细菌产生并分泌一种1350 D分子，该分子对埃及伊蚊幼虫具有皮克级的口服致死性。 该毒素对其它昆虫和动物无毒，用丙酮沉淀生长液，然后用离子交换和反相色谱纯化。 该毒素在含有甘油作为碳源和能源以及硫酸铵作为N源的确定成分培养基中产生。 将从含有C13甘油和N15铵的培养基中制备毒素，用于NMR分析。