MURINE B LYMPHOCYTE DEVELOPMENT

这项研究项目的目的是考察 造血祖细胞产生特定抗原反应性B细胞 收养转移中的子集。一种新的移植模式将是 使用未经辐射的免疫缺陷的XID小鼠，这些小鼠不能 建立抗磷脂酰胆碱(PC)PFC反应。通过使用未辐照的 移植的供体细胞和宿主之间的竞争 完整的宿主细胞为发育成成熟状态提供了机会 在个体发育中应接近正常情况；通过研究 干细胞分化在相对正常的或非破坏性的情况下， 条件，即使是细微的区别之间的再生能力 不同抗原反应亚群的前体将被强调。 因此，该实验系统提供了一种灵敏的手段来分析 新兴B细胞亚群的种群动态。此外，通过 利用PC系统，根据以下条件划分响应的能力 显性和非显性独特型将提供额外的水平 歧视。干细胞移植到精确度和 最小限度地改变环境，以及对相关的、 独一无二的明确定义的抗原反应亚集，由 植入的细胞将不仅提供有关生成和分化的信息 B细胞谱系的多样化，也与克隆性有关 在功能上划定的B细胞隔间内的关系。我们 相信我们的实验将提供关于正常和 异常的小鼠淋巴细胞生成。

The purpose of this research project is to examine the capacities of lymphopoietic progenitors to generate certain antigen-reactive B cell subsets in adoptive transfer. A novel transplantation model will be employed involving unirradiated immunodeficient xid mice which are unable to mount anti-phosphorylcholine (PC) PFC responses. By using unirradiated hosts, the forced competition between the engrafted donor cells and the intact host cells for the opportunity to develop into the mature state should closely approximate the normal situation in ontogeny; by studying stem cell differentiation under relatively normal, or nondisruptive, conditions, even subtle distinctions between the regeneration abilities of precursors to different antigen-reactive subsets will be accentuated. Thus, the experimental system offers a sensitive means for analyzing the population dynamics of emerging B cell subpopulations. Furthermore, by utilizing the PC system, the ability to partition responses in terms of dominant and nondominant idiotypes will provide an additional level of discrimination. The transplantation of stem cells into precisely and minimally altered environments, and the monitoring of the related, idiotypically well-defined antigen-reactive subsets generated by the engrafted cells will provide information not only on the generation and diversification of B cell lineages, but also with respect to the clonal relationships within functionally delineated B cell compartments. We believe that our experiments will provide new information on normal and abnormal murine lymphopoiesis.