THE ROLE OF CALCIUM IN SECRETION FROM TUMOR BASOPHILS

钙在肿瘤嗜碱性粒细胞刺激-分泌偶联中的作用 被研究。其目的将是确定 免疫球蛋白E受体(IgE)的聚集导致 胞浆钙与随后的组胺、5-羟色胺和 其他即刻过敏症的调节剂。 同位素示踪测量~(45)Ca的吸收、外流和运动 均衡将被用来确定 细胞内钙储存，质膜挤出机制和 渗透性途径。因为细胞内pH和膜电位可以 也会影响细胞对钙的处理，这些都将使用 放射性标记离子分布法。 细胞内钙缓冲和荧光指示剂Quin2将是 用于监测细胞质中游离钙离子的变化。会的 也被用来产生一个巨大的细胞质缓冲钙池 ~(45)Ca跨质膜通量的研究 长期的目标是研究钙的分子特征。 通透性途径或通道及其与受体的相互作用 伊格。这应该会加深我们对生物化学和细胞的理解 刺激-分泌耦合生物学和BE在设计方法中的价值 以及治疗过敏和哮喘的药物。

The role of calcium in stimulus-secretion coupling in tumour basophils will be studied. The aim will be to determine the mechanism by which the aggregation of receptors for immunoglobulin E(IgE) leads to an elevation of cytoplasmic Ca++ and the subsequent secretion of histamine, serotonin and other mediators of immediate hypersensitivity. Tracer measurements of 45Ca uptake, efflux and movements at isotopic equilibrium will be used to determine the relative contributions of intracellular calcium stores, plasma membrane extrusion mechanisms and permeability pathways. Since intracellular pH and membrane potential may also affect calcium handling by the cells, these will be measured using radiolabelled ion distribution methods. The intracellular calcium buffer and fluorescence indicator, quin2, will be used to monitor changes in free ionized calcium in the cytoplasm. It will also be used to generate a large cytoplasmic pool of buffered calcium in studies of 45Ca fluxes acrosss the plasma membrane. The long-term objective is the molecular characterization of the calcium permeability pathway or channel and its interactions with receptors for IgE. This should further our understanding of the biochemistry and cell biology of stimulus-secretion coupling and be of value in designing methods and drugs for the treatment of allergies and asthma.