THE ROLE OF CALCIUM IN SECRETION FROM TUMOR BASOPHILS

钙在肿瘤嗜碱性粒细胞分泌中的作用

• 批准号: 3129369
• 负责人: Clare Fewtrell
• 金额: $ 12.78万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-01-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3129369
• 关键词: acid base balance; arachidonate; basophils; calcium; calcium channel blockers; cytoplasm; histamine; hybridomas; hypersensitivity desensitization; immediate hypersensitivity; immunoglobulin E; ion transport; membrane permeability; membrane potentials; mitochondria; multiple myeloma; neoplastic cell; radiotracer; secretion; serotonin

The role of calcium in stimulus-secretion coupling in tumour basophils will be studied. The aim will be to determine the mechanism by which the aggregation of receptors for immunoglobulin E(IgE) leads to an elevation of cytoplasmic Ca++ and the subsequent secretion of histamine, serotonin and other mediators of immediate hypersensitivity. Tracer measurements of 45Ca uptake, efflux and movements at isotopic equilibrium will be used to determine the relative contributions of intracellular calcium stores, plasma membrane extrusion mechanisms and permeability pathways. Since intracellular pH and membrane potential may also affect calcium handling by the cells, these will be measured using radiolabelled ion distribution methods. The intracellular calcium buffer and fluorescence indicator, quin2, will be used to monitor changes in free ionized calcium in the cytoplasm. It will also be used to generate a large cytoplasmic pool of buffered calcium in studies of 45Ca fluxes acrosss the plasma membrane. The long-term objective is the molecular characterization of the calcium permeability pathway or channel and its interactions with receptors for IgE. This should further our understanding of the biochemistry and cell biology of stimulus-secretion coupling and be of value in designing methods and drugs for the treatment of allergies and asthma.

钙在肿瘤嗜碱性粒细胞刺激分泌偶联中的作用将 被研究。 其目的是确定 免疫球蛋白E（IgE）受体的聚集导致 细胞质Ca++和随后分泌的组胺，血清素和 速发型超敏反应的其他介质。 同位素示踪法测量~（45）Ca的摄取、流出和运动 平衡将被用来确定相对贡献 细胞内钙储存，质膜挤出机制， 渗透途径 由于细胞内pH和膜电位可能 也影响细胞对钙的处理，这些将使用 放射性标记离子分布方法。 细胞内钙缓冲液和荧光指示剂quin 2将被 用于监测细胞质中游离离子钙的变化。 它将 也可用于产生缓冲钙的大细胞质池， ~（45）Ca跨膜通量的研究。 长期目标是钙离子的分子特征 渗透途径或通道及其与受体的相互作用， IgE。 这将进一步加深我们对生物化学和细胞的理解。 刺激-分泌偶联的生物学并在设计方法中具有价值 以及治疗过敏和哮喘的药物。

项目成果

The effect of mitochondrial inhibitors on calcium homeostasis in tumor mast cells.

线粒体抑制剂对肿瘤肥大细胞钙稳态的影响。

• DOI: 10.1152/ajpcell.1990.258.2.c217
• 发表时间: 1990
• 期刊: The American journal of physiology
• 作者: Mohr,FC;Fewtrell,C
• 通讯作者: Fewtrell,C

Release of calcium from intracellular stores in rat basophilic leukemia cells monitored with the fluorescent probe chlortetracycline.

用荧光探针金霉素监测大鼠嗜碱性白血病细胞内钙的释放。

• DOI: 10.1002/jcp.1041420111
• 发表时间: 1990
• 期刊: Journal of cellular physiology
• 影响因子: 5.6
• 作者: Marcotte,GV;Millard,PJ;Fewtrell,C
• 通讯作者: Fewtrell,C

IgE receptor-mediated depolarization of rat basophilic leukemia cells measured with the fluorescent probe bis-oxonol.

使用荧光探针 bis-oxonol 测量 IgE 受体介导的大鼠嗜碱性白血病细胞去极化。

• 发表时间: 1987
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Mohr,FC;Fewtrell,C
• 通讯作者: Fewtrell,C

The relative contributions of extracellular and intracellular calcium to secretion from tumor mast cells. Multiple effects of the proton ionophore carbonyl cyanide m-chlorophenylhydrazone.

细胞外和细胞内钙对肿瘤肥大细胞分泌的相对贡献。

• 发表时间: 1987
• 期刊: The Journal of biological chemistry
• 作者: Mohr,FC;Fewtrell,C
• 通讯作者: Fewtrell,C

Depolarization of rat basophilic leukemia cells inhibits calcium uptake and exocytosis.

大鼠嗜碱性白血病细胞的去极化抑制钙摄取和胞吐作用。

• DOI: 10.1083/jcb.104.3.783
• 发表时间: 1987
• 期刊: The Journal of cell biology
• 作者: Mohr,FC;Fewtrell,C
• 通讯作者: Fewtrell,C