THE ROLE OF CALCIUM IN SECRETION FROM TUMOR BASOPHILS

钙在肿瘤嗜碱性粒细胞刺激分泌偶联中的作用将 被研究。 其目的是确定 免疫球蛋白E（IgE）受体的聚集导致 细胞质Ca++和随后分泌的组胺，血清素和 速发型超敏反应的其他介质。 同位素示踪法测量~（45）Ca的摄取、流出和运动 平衡将被用来确定相对贡献 细胞内钙储存，质膜挤出机制， 渗透途径 由于细胞内pH和膜电位可能 也影响细胞对钙的处理，这些将使用 放射性标记离子分布方法。 细胞内钙缓冲液和荧光指示剂quin 2将被 用于监测细胞质中游离离子钙的变化。 它将 也可用于产生缓冲钙的大细胞质池， ~（45）Ca跨膜通量的研究。 长期目标是钙离子的分子特征 渗透途径或通道及其与受体的相互作用， IgE。 这将进一步加深我们对生物化学和细胞的理解。 刺激-分泌偶联的生物学并在设计方法中具有价值 以及治疗过敏和哮喘的药物。

The role of calcium in stimulus-secretion coupling in tumour basophils will be studied. The aim will be to determine the mechanism by which the aggregation of receptors for immunoglobulin E(IgE) leads to an elevation of cytoplasmic Ca++ and the subsequent secretion of histamine, serotonin and other mediators of immediate hypersensitivity. Tracer measurements of 45Ca uptake, efflux and movements at isotopic equilibrium will be used to determine the relative contributions of intracellular calcium stores, plasma membrane extrusion mechanisms and permeability pathways. Since intracellular pH and membrane potential may also affect calcium handling by the cells, these will be measured using radiolabelled ion distribution methods. The intracellular calcium buffer and fluorescence indicator, quin2, will be used to monitor changes in free ionized calcium in the cytoplasm. It will also be used to generate a large cytoplasmic pool of buffered calcium in studies of 45Ca fluxes acrosss the plasma membrane. The long-term objective is the molecular characterization of the calcium permeability pathway or channel and its interactions with receptors for IgE. This should further our understanding of the biochemistry and cell biology of stimulus-secretion coupling and be of value in designing methods and drugs for the treatment of allergies and asthma.