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The sRNA Workbench

sRNA 工作台

基本信息

批准号：
BB/I00016X/1
负责人：
Vincent Moulton
金额：
$ 42.63万
依托单位：
University of East Anglia
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2010
资助国家：
英国
起止时间：
2010 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FI00016X%2F1
关键词：
sRNA Workbench

项目摘要

RNA silencing is a complex and highly conserved regulatory mechanism that is now known to be involved in diverse processes such as development, pathogen control, genome maintenance and response to environmental change. Since its recent discovery, RNA silencing has become a fast moving area of research of great importance in both plant and animal molecular biology. Its discovery has not only been a major breakthrough for our understanding of complex regulatory processes in living cells, but has also opened the door to the development of novel therapeutics for human diseases. The importance of these findings was highlighted by the award of the Nobel prize for medicine in 2006 for the discovery of RNA interference. Small RNA molecules (sRNAs) play a crucial role in the RNA silencing machinery in that they can interact with other nucleic acids in a sequence specific manner to mark them as targets for inactivation. To learn more about this mechanism and its implications, researchers employ cutting-edge sequencing technologies to obtain large datasets of sRNA sequences (typically between 1-10 million sequences about 20-30 nucleotides in length) from a number of organisms. With these technologies becoming more accessible and affordable it is now possible to obtain multiple large-scale data sets which allow the analysis of changes in the sRNA population. These changes can indicate the involvement of RNA silencing pathways in the biological process of interest, such as regulation of key developmental stages in an organism. However, there is a lack of software tools for the analysis of such experiments, which presents a major bottleneck to the exploitation of the technology, especially for biologists without direct bioinformatics support. In this project, we will build on our existing publicly available software tools for sRNA analysis, with the aim of providing an easy to use, downloadable workbench to provide a complete solution for the analysis of plant and animal sRNA datasets. This will provide biologists with a much needed resource to identify novel sRNAs and their function as part of wet-lab studies.

RNA沉默是一种复杂且高度保守的调控机制，目前已知其参与多种过程，如发育、病原体控制、基因组维持和对环境变化的响应。RNA沉默自最近被发现以来，已成为植物和动物分子生物学中非常重要的快速发展的研究领域。它的发现不仅是我们对活细胞中复杂调控过程的理解的重大突破，而且也为人类疾病的新疗法的开发打开了大门。这些发现的重要性在2006年因RNA干扰的发现而获得诺贝尔医学奖时得到了强调。小RNA分子（sRNA）在RNA沉默机制中起着至关重要的作用，因为它们可以以序列特异性方式与其他核酸相互作用，将它们标记为失活的靶标。为了更多地了解这一机制及其影响，研究人员采用尖端测序技术从许多生物体中获得sRNA序列的大型数据集（通常在1- 1000万个序列之间，长度约为20-30个核苷酸）。随着这些技术变得越来越容易获得和负担得起，现在可以获得多个大规模数据集，从而可以分析sRNA群体的变化。这些变化可以指示RNA沉默途径参与感兴趣的生物过程，例如生物体中关键发育阶段的调节。然而，缺乏用于分析此类实验的软件工具，这对该技术的开发提出了主要瓶颈，特别是对于没有直接生物信息学支持的生物学家。在这个项目中，我们将建立在我们现有的公开可用的sRNA分析软件工具，目的是提供一个易于使用，可下载的工作台，为植物和动物sRNA数据集的分析提供一个完整的解决方案。这将为生物学家提供急需的资源，以确定新的sRNA及其功能，作为湿实验室研究的一部分。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The UEA sRNA workbench: a suite of tools for analysing and visualizing next generation sequencing microRNA and small RNA datasets.

DOI：
10.1093/bioinformatics/bts311
发表时间：
2012-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
0
作者：
Stocks MB;Moxon S;Mapleson D;Woolfenden HC;Mohorianu I;Folkes L;Schwach F;Dalmay T;Moulton V
通讯作者：
Moulton V

Global discovery and characterization of small non-coding RNAs in marine microalgae.

海洋微藻中小非编码 RNA 的全球发现和表征。

DOI：
10.1186/1471-2164-15-697
发表时间：
2014-08-20
期刊：
BMC genomics
影响因子：
4.4
作者：
Lopez-Gomollon S;Beckers M;Rathjen T;Moxon S;Maumus F;Mohorianu I;Moulton V;Dalmay T;Mock T
通讯作者：
Mock T

FiRePat-Finding Regulatory Patterns between sRNAs and Genes

FiRePat-寻找 sRNA 和基因之间的调控模式