IMMUNOLOGIC STUDIES IN ECTOTHERMIC VERTEBRATES

拟议研究的主要重点是更好地了解 体温外动物免疫反应的细胞方面。《博大》 这项研究的目标有三个。首先，建议对其进行测试 鱼类有两类与T类似的淋巴细胞的假说 哺乳动物和鸟类的B细胞。检验这一假设将涉及到 利用抗鲶鱼单抗的细胞分离技术 淋巴细胞表面成分与淋巴细胞体外检测相结合 功能。第二个目标是检验Low 鱼的温度免疫抑制主要是由于偏爱 对辅助性细胞生成的影响。检验这一假说将 包括体内和体外温度影响的研究。 T依赖性和非T依赖性抗体分泌细胞的产生 抗原。第三个目标是检验鱼类T细胞的假设 具有与鱼类B细胞不同的膜成分，因此，没有 适当的低温驯化(同质粘性适应)，不会 在较低的温度下进行有丝分裂反应，鱼类B细胞也是如此。 验证这一假说将涉及对分离的叉尾叉尾鱼B和T的研究 细胞质膜与有丝分裂过程中的细胞事件 在不同温度下的响应。乐观地说，这些拟议的研究 将产生关于系统发育的重要信息 免疫反应和激活的基本特征， 淋巴细胞的分化和相互作用。

The major emphasis of the proposed research is to better understand the cellular aspects of immune responses in ectothermic animals. The broad objectives of the research are threefold. Firstly it is proposed to test the hypothesis that fish have two classes of lymphocytes comparable to T and B cells of mammals and birds. Testing this hypothesis will involve cell separation techniques employing monoclonal antibodies to catfish lymphocyte surface components combined with in vitro assays of lymphocyte functions. The second objective is to test the hypothesis that low temperature immunosupression in fish results primarly from a preferential effect on the generation of helper cells. Testing this hypothesis will involve in vivo and in vitro studies of temperature effects on the generation of antibody secreting cells to T-dependent an T-independent antigens. The third objective is to test the hypothesis that fish T cells have a different membrane composition than fish B cells and hence, without appropriate low temperature acclimation (homeoviscous adaptation), will not undergo mitogenic responses at lower temperatures as do fish B cells. Testing this hypothesis will involve studies on isolated catfish B and T cell plasma membranes and on cellular events occurring during mitogenic responses at various temperatures. Optimistically, these proposed studies will result in important information regarding the phylogenetic development of immune responses and the fundamental features of the activation, differentiation and interaction of lymphocytes.