Understanding programming in highly reducing iterative fungal polyketide synthases - a structural and mechanistic approach

了解高度还原迭代真菌聚酮合酶的编程——一种结构和机制方法

• 批准号: BB/I003355/1
• 负责人: Thomas Simpson
• 金额: $ 87.23万
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI003355%2F1
• 关键词: Understanding; programming; highly; reducing; iterative

Fungal polyketide synthases (PKS) are extremely large multifunctional multi-domain proteins that assemble simple biochemical building blocks in a highly programmed iterative manner to make a huge range of biologically active complex natural products many of which are of importance as drugs or agrochemical agents or as toxins which are detrimental to both human health and agriculture. In simple terms PKSs are like beads on a string where each bead or 'domain' of the PKS represents an individual enzyme function or biological catalyst. The PKS uses simple carbon-carbon bond forming (condensation) reactions followed by a variety of 'tailoring' reactions in which the initial condensation product may be modified by all or some of a series of reactions - reduction (addition of two atoms of hydrogen), dehydration (loss of water) and C-methylation (addition of a single carbon branch), each of which is controlled by specific domains on the PKS. Discovering the molecular factors which control this programming is arguably the greatest remaining problem in our understanding of how nature makes complex natural products, and also represents a great fundamental problem in enzyme catalysis. Understanding of how the programming works, how the number iterative condensation cycle that determines the overall chain length, degree of reduction, and methylation is essentially absent. In this project the individual beads/domains will be isolated so that their individual properties and structures can be studied. Individual beads will then be brought together to study how one influences another, and this process will be repeated to try to build up an overall picture of how the intact PKS works. The project is highly interdisciplinary and will bring together the skills and expertise of natural products chemistry, chemical synthesis, molecular biology, biochemistry, structural biology and bioinformatics. The ultimate aim is to use the understanding gained of how the programming actually works, to manipulate the PKSs to make new products with altered or improved properties of benefit to human and animal health and to provide the pharmaceutical and fine chemical industries with new methods to produce new useful products.

真菌聚酮酶（PKS）是一种非常大的多功能多结构域蛋白，其以高度程序化的迭代方式组装简单的生物化学构件，以产生大量具有生物活性的复杂天然产物，其中许多是重要的药物或农用化学剂或毒素，其对人类健康和农业都有害。简单地说，PKS就像一串珠子，其中PKS的每个珠子或“结构域”代表单个酶功能或生物催化剂。PKS使用简单的碳-碳键形成（缩合）反应，然后是各种“定制”反应，其中初始缩合产物可以通过一系列反应中的全部或一些进行修饰-还原（添加两个氢原子）、脱水（失水）和C-甲基化（添加单个碳分支），其中每一个都由PKS上的特定结构域控制。发现控制这种编程的分子因素可以说是我们理解自然界如何制造复杂天然产物的最大遗留问题，也代表了酶催化的一个重要基本问题。了解编程如何工作，如何确定总链长，还原程度和甲基化的迭代缩合循环的数量基本上是不存在的。在本项目中，将分离单个珠/域，以便研究其各自的特性和结构。然后，将单个珠子放在一起，研究一个珠子如何影响另一个珠子，重复这个过程，试图建立完整的PKS如何工作的整体画面。该项目是高度跨学科的，将汇集天然产物化学，化学合成，分子生物学，生物化学，结构生物学和生物信息学的技能和专业知识。最终目的是利用对编程实际工作方式的理解，操纵PKS以制造具有改变或改善的有益于人类和动物健康的特性的新产品，并为制药和精细化工行业提供生产新有用产品的新方法。

项目成果

Structural and synthetic studies on maleic anhydride and related diacid natural products

• DOI: 10.1016/j.tet.2019.130717
• 发表时间: 2020-01-03
• 期刊: TETRAHEDRON
• 影响因子: 2.1
• 作者: Heard, David M.;Tayler, Emyr R.;Willis, Christine L.
• 通讯作者: Willis, Christine L.

Oxidative dearomatisation: the key step of sorbicillinoid biosynthesis†Electronic supplementary information (ESI) available: Containing all experimental details. See DOI: 10.1039/c3sc52911hClick here for additional data file.

• DOI: 10.1039/c3sc52911h
• 发表时间: 2014-02-23
• 期刊: Chemical science
• 影响因子: 8.4
• 作者: Fahad AA;Abood A;Fisch KM;Osipow A;Davison J;Avramović M;Butts CP;Piel J;Simpson TJ;Cox RJ
• 通讯作者: Cox RJ

Kinetic characterisation of the FAD dependent monooxygenase TropB and investigation of its biotransformation potential

• DOI: 10.1039/c5ra06693j
• 发表时间: 2015-01-01
• 期刊: RSC ADVANCES
• 影响因子: 3.9
• 作者: Abood, Amira;Al-Fahad, Ahmed;Cox, Russell J.
• 通讯作者: Cox, Russell J.

Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein.

• DOI: 10.1039/c5sc03864b
• 发表时间: 2016-03-01
• 期刊: Chemical science
• 影响因子: 8.4
• 作者: Dong X;Bailey CD;Williams C;Crosby J;Simpson TJ;Willis CL;Crump MP
• 通讯作者: Crump MP