MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
批准号:
3131248
负责人:
DONNA M PAULNOCK
金额:
$10.85万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-07-01 至 1991-06-30
关键词:
T lymphocyte affinity chromatography antigen antibody reaction antigens antiidiotype antibody bactericidal immunity bioassay cell cell interaction cell population study flow cytometry gel electrophoresis genetic library genetic manipulation histocompatibility antigens immunochemistry immunofluorescence technique immunogenetics interferons laboratory mouse laboratory rat leukocyte activation /transformation lymphokines macrophage molecular cloning monoclonal antibody neoplasm /cancer immunology phagocytes protein structure radioimmunoassay surface antigens transfection
中文摘要
单核巨噬细胞系统的细胞是相关的
具有多种与免疫反应相关的功能和
活动。这些措施包括参与东道主防御
肿瘤和感染性生物体,以及在积极和
对免疫反应的消极控制。的强化作用
巨噬细胞的活性在这种功能的发展中是
至少部分地由过程中产生的可溶性介体调节
免疫反应的过程。有一个因素被证明具有
强大的巨噬细胞激活能力是干扰素-γ;
然而,这一因素控制的机制
巨噬细胞的功能仍有待阐明。该计划的目标是
建议的研究是为了了解分子
巨噬细胞激活的基础,通过免疫化学
与激活相关的细胞表面变化的分析
干扰素对巨噬细胞的影响。利用已建立的巨噬细胞
免疫品系,异种单抗探针
将发展成细胞表面抗原,这些抗原是在
用免疫干扰素治疗巨噬细胞。这些
然后将使用抗体对表面进行详细分析
慢性阻塞性肺疾病中期巨噬细胞的抗原表型
激活途径,并用于鉴定特定抗原
它们在杀瘤活性或抗原呈递中起作用
激活细胞的细胞功能。抗体也将被使用,
与生化技术相结合,检测和纯化
功能相关的分子基因产物
人物刻画。这种抗体最终可能对
巨噬细胞选择性操作的实用水平
在体内发挥作用。
英文摘要
The cells of the mononuclear phagocyte system are associated
with a wide variety of immune response-related functions and
activities. These include a participation in host defense against
neoplasia and infectious organisms, and a role in the positive and
negative control of immune responses. The potentiation of
macrophage activity in the development of such functions is
regulated, at least in part, by soluble mediators produced during
the course of an immune response. One factor shown to have
potent macrophage activating capability is interferon-gamma;
however, the mechanisms by which this factor controls
macrophage function remain to be elucidate. The objective of the
proposed research is to gain an understanding of the molecular
basis of macrophage activation, through the immunochemical
analysis of cell surface alterations associated with the activation
of macrophages by interferon. Using established macrophage cell
lines for immunization, xenogeneic monoclonal antibody probes
will be developed to cell surface antigens which are induced on
macrphages by treatment with immune interferon. These
antibodies will then be used in a detailed analysis of the surface
antigen phenotype of macrophages at intermediate stages in the
pathway of activation, and for identification of specific antigens
which play a role in the tumoricidal activity or antigen-presenting
cell function of activated cells. The antibodies will also be used,
in combination with biochemical techniques, to detect and purify
functionally relevant gene products for molecular
characterization. Such antibodies ultimately may be useful on a
practical level in the selective manipulation of macrophage
function in vivo.
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Transforming growth factor-beta 1 inhibits activation of macrophage cell line RAW 264.7 for cell killing.
转化生长因子-β 1 抑制巨噬细胞系 RAW 264.7 的活化以杀死细胞。
DOI:
10.1111/j.1365-2249.1990.tb05461.x
发表时间:
1990
期刊:
Clinical and experimental immunology
影响因子:
4.6
作者:
[Haak-Frendscho,M, Wynn,TA, Czuprynski,CJ, Paulnock,D]
通讯作者:
Paulnock,D
Identification and characterization of monoclonal antibodies specific for macrophages at intermediate stages in the tumoricidal activation pathway.
杀肿瘤激活途径中间阶段巨噬细胞特异性单克隆抗体的鉴定和表征。
DOI:
--
发表时间:
1990
期刊:
Journal of immunology (Baltimore, Md. : 1950)
影响因子:
--
作者:
[Paulnock,DM, Lambert,LE]
通讯作者:
Lambert,LE
Induced proteins in human peripheral mononuclear cells over a range of clinically tolerable doses of interferon-gamma.
在一系列临床可耐受剂量的干扰素-γ 下,在人外周单核细胞中诱导蛋白质。
DOI:
10.1089/jir.1989.9.457
发表时间:
1989
期刊:
Journal of interferon research
影响因子:
--
作者:
[Paulnock,DM, Havlin,KA, Storer,BM, Spear,GT, Sielaff,KM, Borden,EC]
通讯作者:
Borden,EC
Modulation of macrophage function by gamma-irradiation. Acquisition of the primed cell intermediate stage of the macrophage tumoricidal activation pathway.
通过伽马射线照射调节巨噬细胞功能。
DOI:
--
发表时间:
1987
期刊:
Journal of immunology (Baltimore, Md. : 1950)
影响因子:
--
作者:
[Lambert,LE, Paulnock,DM]
通讯作者:
Paulnock,DM
DIRECTOR'S OFFICE
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批准号:8173063
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项目类别:
-
资助金额:$10.33万
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财政年份:2010
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负责人:DONNA M PAULNOCK
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依托单位:
WISCONSIN NATIONAL PRIMATE RESEARCH CENTER SUPPORT
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批准号:8173147
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资助金额:$5.16万
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Modulation of innate immunity by microbial factors
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批准号:6463236
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资助金额:$18.19万
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依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
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批准号:6434265
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项目类别:
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资助金额:$32.53万
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Modulation of innate immunity by microbial factors
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批准号:6888539
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资助金额:$18.19万
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依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
-
批准号:6838220
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项目类别:
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资助金额:$32.5万
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依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
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资助金额:$32.52万
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MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
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批准号:6698013
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资助金额:$32.51万
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财政年份:2002
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Modulation of innate immunity by microbial factors
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项目类别:
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资助金额:$17.76万
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Modulation of innate immunity by microbial factors
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批准号:6623118
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项目类别:
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资助金额:$18.19万
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财政年份:2002
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负责人:DONNA M PAULNOCK
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依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
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批准号:7013654
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项目类别:
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资助金额:$31.74万
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Modulation of innate immunity by microbial factors
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批准号:6746890
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项目类别:
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资助金额:$18.19万
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财政年份:2002
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负责人:DONNA M PAULNOCK
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依托单位:
GENE REGULATION DURING MACROPHAGE ACTIVATION
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批准号:2099649
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项目类别:
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资助金额:$9.7万
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财政年份:1995
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负责人:DONNA M PAULNOCK
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GENE REGULATION DURING MACROPHAGE ACTIVATION
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批准号:2099650
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资助金额:$10.08万
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财政年份:1995
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依托单位:
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项目类别:
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财政年份:1995
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负责人:DONNA M PAULNOCK
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依托单位:
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
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批准号:3131243
-
项目类别:
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资助金额:$12.21万
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财政年份:1984
-
负责人:DONNA M PAULNOCK
-
依托单位:
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
-
批准号:3131247
-
项目类别:
-
资助金额:$11.28万
-
财政年份:1984
-
负责人:DONNA M PAULNOCK
-
依托单位:
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
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批准号:3131245
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项目类别:
-
资助金额:$7.33万
-
财政年份:1984
-
负责人:DONNA M PAULNOCK
-
依托单位:
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
-
批准号:3131246
-
项目类别:
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资助金额:$6.73万
-
财政年份:1984
-
负责人:DONNA M PAULNOCK
-
依托单位:
海外基金