MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
批准号:
3131248
负责人:
DONNA M PAULNOCK
金额:
$10.85万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-07-01 至 1991-06-30
关键词:
T lymphocyte affinity chromatography antigen antibody reaction antigens antiidiotype antibody bactericidal immunity bioassay cell cell interaction cell population study flow cytometry gel electrophoresis genetic library genetic manipulation histocompatibility antigens immunochemistry immunofluorescence technique immunogenetics interferons laboratory mouse laboratory rat leukocyte activation /transformation lymphokines macrophage molecular cloning monoclonal antibody neoplasm /cancer immunology phagocytes protein structure radioimmunoassay surface antigens transfection
中文摘要
单核吞噬细胞系统的细胞相互关联
英文摘要
The cells of the mononuclear phagocyte system are associated
with a wide variety of immune response-related functions and
activities. These include a participation in host defense against
neoplasia and infectious organisms, and a role in the positive and
negative control of immune responses. The potentiation of
macrophage activity in the development of such functions is
regulated, at least in part, by soluble mediators produced during
the course of an immune response. One factor shown to have
potent macrophage activating capability is interferon-gamma;
however, the mechanisms by which this factor controls
macrophage function remain to be elucidate. The objective of the
proposed research is to gain an understanding of the molecular
basis of macrophage activation, through the immunochemical
analysis of cell surface alterations associated with the activation
of macrophages by interferon. Using established macrophage cell
lines for immunization, xenogeneic monoclonal antibody probes
will be developed to cell surface antigens which are induced on
macrphages by treatment with immune interferon. These
antibodies will then be used in a detailed analysis of the surface
antigen phenotype of macrophages at intermediate stages in the
pathway of activation, and for identification of specific antigens
which play a role in the tumoricidal activity or antigen-presenting
cell function of activated cells. The antibodies will also be used,
in combination with biochemical techniques, to detect and purify
functionally relevant gene products for molecular
characterization. Such antibodies ultimately may be useful on a
practical level in the selective manipulation of macrophage
function in vivo.
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Transforming growth factor-beta 1 inhibits activation of macrophage cell line RAW 264.7 for cell killing.
转化生长因子-β 1 抑制巨噬细胞系 RAW 264.7 的活化以杀死细胞。
DOI:
10.1111/j.1365-2249.1990.tb05461.x
发表时间:
1990
期刊:
Clinical and experimental immunology
影响因子:
4.6
作者:
[Haak-Frendscho,M, Wynn,TA, Czuprynski,CJ, Paulnock,D]
通讯作者:
Paulnock,D
Identification and characterization of monoclonal antibodies specific for macrophages at intermediate stages in the tumoricidal activation pathway.
杀肿瘤激活途径中间阶段巨噬细胞特异性单克隆抗体的鉴定和表征。
DOI:
--
发表时间:
1990
期刊:
Journal of immunology (Baltimore, Md. : 1950)
影响因子:
--
作者:
[Paulnock,DM, Lambert,LE]
通讯作者:
Lambert,LE
Induced proteins in human peripheral mononuclear cells over a range of clinically tolerable doses of interferon-gamma.
在一系列临床可耐受剂量的干扰素-γ 下,在人外周单核细胞中诱导蛋白质。
DOI:
10.1089/jir.1989.9.457
发表时间:
1989
期刊:
Journal of interferon research
影响因子:
--
作者:
[Paulnock,DM, Havlin,KA, Storer,BM, Spear,GT, Sielaff,KM, Borden,EC]
通讯作者:
Borden,EC
Modulation of macrophage function by gamma-irradiation. Acquisition of the primed cell intermediate stage of the macrophage tumoricidal activation pathway.
通过伽马射线照射调节巨噬细胞功能。
DOI:
--
发表时间:
1987
期刊:
Journal of immunology (Baltimore, Md. : 1950)
影响因子:
--
作者:
[Lambert,LE, Paulnock,DM]
通讯作者:
Paulnock,DM
DIRECTOR'S OFFICE
-
批准号:8173063
-
项目类别:
-
资助金额:$10.33万
-
财政年份:2010
-
负责人:DONNA M PAULNOCK
-
依托单位:
WISCONSIN NATIONAL PRIMATE RESEARCH CENTER SUPPORT
-
批准号:8173147
-
项目类别:
-
资助金额:$5.16万
-
财政年份:2010
-
负责人:DONNA M PAULNOCK
-
依托单位:
Modulation of innate immunity by microbial factors
-
批准号:6463236
-
项目类别:
-
资助金额:$18.19万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
-
批准号:6434265
-
项目类别:
-
资助金额:$32.53万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
-
批准号:6621422
-
项目类别:
-
资助金额:$32.52万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
-
批准号:6698013
-
项目类别:
-
资助金额:$32.51万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
-
批准号:6838220
-
项目类别:
-
资助金额:$32.5万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
Modulation of innate immunity by microbial factors
-
批准号:6888539
-
项目类别:
-
资助金额:$18.19万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
Modulation of innate immunity by microbial factors
-
批准号:7067148
-
项目类别:
-
资助金额:$17.76万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
Modulation of innate immunity by microbial factors
-
批准号:6623118
-
项目类别:
-
资助金额:$18.19万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
MACROPHAGE ACTIVATION IN AFRICAN TRYPANOSOMIASIS
-
批准号:7013654
-
项目类别:
-
资助金额:$31.74万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
Modulation of innate immunity by microbial factors
-
批准号:6746890
-
项目类别:
-
资助金额:$18.19万
-
财政年份:2002
-
负责人:DONNA M PAULNOCK
-
依托单位:
GENE REGULATION DURING MACROPHAGE ACTIVATION
-
批准号:2099649
-
项目类别:
-
资助金额:$9.7万
-
财政年份:1995
-
负责人:DONNA M PAULNOCK
-
依托单位:
GENE REGULATION DURING MACROPHAGE ACTIVATION
-
批准号:2099650
-
项目类别:
-
资助金额:$10.08万
-
财政年份:1995
-
负责人:DONNA M PAULNOCK
-
依托单位:
GENE REGULATION DURING MACROPHAGE ACTIVATION
-
批准号:2443023
-
项目类别:
-
资助金额:$10.49万
-
财政年份:1995
-
负责人:DONNA M PAULNOCK
-
依托单位:
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
-
批准号:3131243
-
项目类别:
-
资助金额:$12.21万
-
财政年份:1984
-
负责人:DONNA M PAULNOCK
-
依托单位:
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
-
批准号:3131247
-
项目类别:
-
资助金额:$11.28万
-
财政年份:1984
-
负责人:DONNA M PAULNOCK
-
依托单位:
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
-
批准号:3131245
-
项目类别:
-
资助金额:$7.33万
-
财政年份:1984
-
负责人:DONNA M PAULNOCK
-
依托单位:
MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION
-
批准号:3131246
-
项目类别:
-
资助金额:$6.73万
-
财政年份:1984
-
负责人:DONNA M PAULNOCK
-
依托单位:
海外基金