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MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION

巨噬细胞激活的单克隆抗体分析

基本信息

批准号：
3131248
负责人：
DONNA M PAULNOCK
金额：
$ 10.85万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-07-01 至 1991-06-30
项目状态：
已结题

项目摘要

The cells of the mononuclear phagocyte system are associated with a wide variety of immune response-related functions and activities. These include a participation in host defense against neoplasia and infectious organisms, and a role in the positive and negative control of immune responses. The potentiation of macrophage activity in the development of such functions is regulated, at least in part, by soluble mediators produced during the course of an immune response. One factor shown to have potent macrophage activating capability is interferon-gamma; however, the mechanisms by which this factor controls macrophage function remain to be elucidate. The objective of the proposed research is to gain an understanding of the molecular basis of macrophage activation, through the immunochemical analysis of cell surface alterations associated with the activation of macrophages by interferon. Using established macrophage cell lines for immunization, xenogeneic monoclonal antibody probes will be developed to cell surface antigens which are induced on macrphages by treatment with immune interferon. These antibodies will then be used in a detailed analysis of the surface antigen phenotype of macrophages at intermediate stages in the pathway of activation, and for identification of specific antigens which play a role in the tumoricidal activity or antigen-presenting cell function of activated cells. The antibodies will also be used, in combination with biochemical techniques, to detect and purify functionally relevant gene products for molecular characterization. Such antibodies ultimately may be useful on a practical level in the selective manipulation of macrophage function in vivo.

单核吞噬细胞系统的细胞是相关的具有多种与免疫反应相关的功能活动。其中包括参与宿主防御肿瘤和传染性生物体，以及积极和积极的作用免疫反应的负控制。的增强作用巨噬细胞在发展此类功能时的活动是至少部分地受到过程中产生的可溶性介体的调节免疫反应的过程。一项因素显示出有效的巨噬细胞激活能力是干扰素-γ；然而，该因素控制的机制巨噬细胞的功能仍有待阐明。该计划的目标拟议的研究是为了了解分子巨噬细胞活化的基础，通过免疫化学分析与激活相关的细胞表面变化干扰素对巨噬细胞的影响。使用已建立的巨噬细胞免疫生产线、异种单克隆抗体探针将被开发为细胞表面抗原，这些抗原被诱导通过免疫干扰素治疗巨噬细胞。这些然后抗体将用于对表面进行详细分析巨噬细胞在中间阶段的抗原表型激活途径，并用于识别特定抗原在杀肿瘤活性或抗原呈递中发挥作用活化细胞的细胞功能。抗体也将被使用，结合生化技术进行检测和纯化功能相关的分子基因产物表征。此类抗体最终可能可用于巨噬细胞选择性操纵的实践水平在体内发挥作用。