BIOLOGY OF LEISHMANIA IN PHLEBOTOMINE SANDFLIES

白蛉中利什曼原虫的生物学

• 批准号: 3130958
• 负责人: ROBERT B TESH
• 金额: $ 23.56万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3130958
• 关键词: Cypovirus; Leishmania; Psychodidae; density gradient ultracentrifugation; dietary carbohydrates; disease vectors; electrofocusing; enzyme linked immunosorbent assay; epidemiology; gel electrophoresis; genetic recombination; hamsters; host organism interaction; laboratory mouse; laboratory rabbit; leishmaniasis; life cycle; microorganism genetics; microorganism growth; microorganism immunology; nucleic acid hybridization; nutrient requirement; nutrition related tag; protozoal antigen; tissue /cell culture

The objective of this project is to study the biology of human pathogenic Leishmania in their phlebotomine sandfly vectors. The project involves the disciplines of parasitology, medical entomology, genetics, virology, biochemistry and immunology. Laboratory-reared sandflies will be experimentally infected, with a number of different Leishmania types and the following aspects of the parasites biology in the insects will be studied: the role of sugars in the enhancement or inhibition of leishmanial infection in sandflies, the effect of avian blood on the parasite infection in the vector, the genetic basis of vector competence in sandflies, biochemical and morphological studies of the sandfly hind gut and its role in the transmission of Le. braziliensis group parasites, and the possibility that genetic recombination might occur among related Leishmania types in the insect vector. A newly discovered sandfly cytoplasmic polyhydrosis virus will also be characterized and evaluated as a possible biological control agent for leishmaniasis.

该项目的目的是研究人类的生物学 白蛉载体中存在致病性利什曼原虫。 这 项目涉及寄生虫学、医学等学科 昆虫学、遗传学、病毒学、生物化学和免疫学。 实验室饲养的白蛉将被实验感染， 许多不同的利什曼原虫类型和以下方面 将研究昆虫体内寄生虫的生物学： 糖在增强或抑制利什曼原虫感染中的作用 白蛉，禽血对寄生虫感染的影响 载体，白蛉载体能力的遗传基础， 白蛉后肠的生化和形态学研究 其在传播乐中的作用。巴西亚群寄生虫，和 基因重组的可能性 昆虫媒介中相关的利什曼原虫类型。 一个新的 发现的白蛉胞质多水解病毒也将 作为一种可能的生物防治剂进行表征和评估 对于利什曼病。