SUPPRESSOR T CELLS IN HAPTEN SPECIFIC IMMUNITY

本研究旨在分析H-2与 编码的基因产物和VH样基因产物，其在 抑制性T细胞（Ts）通路。 I-J行列式之间的关系 一级Ts细胞上的独特型元件将在一系列中进行检查 的F1杂种，以检查优先分配I-J和VH结构 其存在于抑制分子（TsF）上。 H-2的遗传基础 在二阶（Ts2）和三阶（Ts3）之间发生的限制 细胞将通过评估遗传限制诱导 Ts3代的信号。 此外，通过在一个实施例中生成Ts2和TsF2， 一组近交系，并检查这样产生的TsF2 在F1杂种或其他近交系中的功能，我们将确定H-2 控制Ts2激活和功能的基因座。 此外，特殊作用 I-J+抗原呈递细胞（APC）的数量将使用体外 在细胞毒性测定中，我们可以选择性地操纵APC活性。 我们 还将描述两种化合物的功能和结构特性， 长期Ts杂交瘤。 我们将评估体外和体内功能 在每一个纯化和表征的水平上。 最后使用 分子生物学方法，我们将评估活性和结构 由纯化和分离的mRNA翻译的蛋白质， 杂交瘤 因此，这些研究的主要目的是分析 Ts细胞及其产物在半抗原特异性免疫中免疫生物学

The objective of this grant is to analyze the relationship between H-2 encoded gene products and VH like gene products which are important in suppressor T cell (Ts) pathways. The relationship between I-J determinants and idiotypic elements on first order Ts cells will be examined in a series of F1 hybrids to examine the preferential assortment I-J and VH structures which are present on suppressor molecules (TsF). The genetic basis of H-2 restriction which occurs between second order (Ts2) and third order (Ts3) cells will be examined by evaluating the genetically restricted inductive signals for Ts3 generation. Moreover, by generating Ts2 and TsF2 in a panel of inbred strains and examining the ability of such generated TsF2 to function in F1 hybrids or other inbred strains, we will determine the H-2 loci which govern Ts2 activation and function. Moreover, the special role of I-J+ antigen presenting cells (APC) will be deduced using an in vitro cytotoxicity assay in which we can selectively manipulate APC activity. We will also characterize the functional and structural properties of two long-term Ts hybridomas. We will evaluate in ivitro and in vivo function at each level of pruification and characterization. Finally using a molecular biological approach, we will evaluate the activity and structure of proteins translated by purified and isolated mRNA obtained from these hybridomas. The major thrust of these studies is therefore to analyze the immunobiology of Ts cells and their products in hapten specific immunity.