SUPPRESSOR T CELLS IN HAPTEN SPECIFIC IMMUNITY

半抗原特异性免疫中的抑制 T 细胞

• 批准号: 3134485
• 负责人: MARK I GREENE
• 金额: $ 17.88万
• 依托单位: TUFTS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134485
• 关键词: cell cell interaction; chemical structure function; genetic manipulation; genetic regulation; histocompatibility gene; hybridomas; immunochemistry; immunogenetics; immunoglobulin genes; immunoregulation; leukocyte activation /transformation; messenger RNA; suppressor T lymphocyte

The objective of this grant is to analyze the relationship between H-2 encoded gene products and VH like gene products which are important in suppressor T cell (Ts) pathways. The relationship between I-J determinants and idiotypic elements on first order Ts cells will be examined in a series of F1 hybrids to examine the preferential assortment I-J and VH structures which are present on suppressor molecules (TsF). The genetic basis of H-2 restriction which occurs between second order (Ts2) and third order (Ts3) cells will be examined by evaluating the genetically restricted inductive signals for Ts3 generation. Moreover, by generating Ts2 and TsF2 in a panel of inbred strains and examining the ability of such generated TsF2 to function in F1 hybrids or other inbred strains, we will determine the H-2 loci which govern Ts2 activation and function. Moreover, the special role of I-J+ antigen presenting cells (APC) will be deduced using an in vitro cytotoxicity assay in which we can selectively manipulate APC activity. We will also characterize the functional and structural properties of two long-term Ts hybridomas. We will evaluate in ivitro and in vivo function at each level of pruification and characterization. Finally using a molecular biological approach, we will evaluate the activity and structure of proteins translated by purified and isolated mRNA obtained from these hybridomas. The major thrust of these studies is therefore to analyze the immunobiology of Ts cells and their products in hapten specific immunity.

这笔资助的目的是分析H-2之间的关系 编码的基因产物和VH样基因产物在 抑制性 T 细胞 (Ts) 途径。 I-J决定因素之间的关系 和一级 T 细胞上的独特型元件将进行一系列检查 F1 杂交体以检查优先分类 I-J 和 VH 结构 它们存在于抑制分子（TsF）上。 H-2的遗传基础 发生在二阶 (Ts2) 和三阶 (Ts3) 之间的限制 将通过评估遗传限制的诱导来检查细胞 Ts3 生成的信号。 此外，通过在a中生成Ts2和TsF2 近交系小组并检查此类生成的 TsF2 的能力 F1 杂种或其他近交系中的功能，我们将确定 H-2 控制 Ts2 激活和功能的基因座。 此外，特殊角色 I-J+ 抗原呈递细胞 (APC) 将使用体外推断 细胞毒性测定，我们可以选择性地操纵 APC 活性。 我们 还将表征两种的功能和结构特性 长期Ts杂交瘤。 我们将评估体外和体内功能 在纯化和表征的每个级别。 最后使用一个 分子生物学方法，我们将评估活性和结构 由从这些获得的纯化和分离的 mRNA 翻译的蛋白质 杂交瘤。 因此，这些研究的主要目的是分析 Ts细胞及其产物在半抗原特异性免疫中的免疫生物学。