IMMUNOPATHOLOGY OF MYCOBACTERIUM INTRACELLULARE IN AIDS

最令人痛苦和潜在的严重健康问题之一 最近在美国人的生活中发现的是获得性免疫缺陷症 综合征（艾滋病）。 在最初不到3年的时间里 认识，许多患者已被发现有这种综合征。 的 疾病的严重性和非常高的痛苦和严峻的事实 死亡率促使一些有关机构和个人发起了一项 与这种疾病的斗争。 艾滋病患者越来越多 易受危及生命的感染，由几种机会性感染引起， 微生物，这做，在最多只造成良性感染过程 在正常人中。 本申请涉及免疫病理学 其中之一的研究，M。胞内的 不像其他 机会微生物M.细胞内感染可引起难治性， 在非艾滋病患者中也存在播散型疾病。 后 从我们的实验室获得的早期线索，米色的适用性 C57 B1/6小鼠作为艾滋病实验动物模型的可行性 患者M.将研究细胞内感染。 其他 除此之外，他们还发现了一些其他的证据。 对这种感染的易感性，是缺乏自然杀伤细胞（NK） 细胞和增加的抑制细胞-确定为常见的特征 在艾滋病患者中。 本研究的一个重要方面是评估 免疫抑制剂和娱乐药物，通常用于艾滋病 患者，在该动物模型中该生物体的发病机制， 通过体内细菌增殖和死亡率来判断。 为了 进一步描述和加强这一模式的要求，具体 通过评估宿主的免疫调节作用， 前列腺素（PGE 2）及其合成， 白细胞介素-2（IL-2）和γ干扰素（IFN）。 保护性研究 使用吲哚美辛（抑制剂或前列腺素合成）、IL-2和 IFN还将补充这些药物的临床研究结果， 已经在这个国家进行。 最后，具体实验旨在 在表征和定量T亚群（T辅助细胞（TH）和T细胞亚群）时， 抑制（TS）细胞）群体和TH：TS细胞比例在这只小鼠 模型，感染M.细胞内研究将进一步加深对 研究这种综合征的病理学， 该模型

One of the most distressing and potentially serious health problems recently discovered in American life is the Acquired Immune Deficiency Syndrome (AIDS). In the short span of less than 3 years after the initial recognition, many patients have been discovered to have this syndrome. The agonizing and grim facts of the seriousness of the disease and very high mortality prompted several concerned agencies and individuals to launch a serious battle against this malady. AIDS patients become increasingly susceptible to life threatening infections, caused by several opportunistic microorganisms, which do, at the most cause only benign infection processes in normal individuals. This application concerns the immunopathologic studies with one of these, M. intracellulare. Unlike the other opportunistic microorganisms, M. intracellulare can cause a refractory, disseminated type of disease in non-AIDS patients as well. Following the earlier leads obtained from our laboratories, the suitability of the Beige mutants of C57B1/6 mice as a possible experimental animal model for AIDs patients with M. intracellulare infections will be investigated. Other attributes of the Beige mice, besides the evidence of their increased susceptibility to this infection, are the absence of natural killer (NK) cells, and increased suppressor cells - features established to be common in AIDS patients. An important aspect in this study is to assess the role of immunosuppressive and recreational drugs, commonly used by the AIDS patients, on the pathogenesis of this organism in this animal model, as judged by in vivo bacterial multiplication and mortality. In order to further characterize and strengthen the claims of this model, specific investigations on the immunomodulation of the host by way of assessment of prostaglandin (PGE2) and synthesis with its consequent influence on Interleukin-2 (IL-2) and gamma interferon (IFN). Protective studies in animals using indomethacin (inhibitor or prostaglandin synthesis), IL-2 and IFN will also complement the results of clinical studies with these agents, already in progress in this country. Finally, specific experiments aimed at characterization and quantitation of the T subset (T helper (TH) and T suppressor (TS) cell) populations and the TH:TS cell ratios in this mouse model, following infection with M. intracellulare will add further insight into the pathobiology of this syndrome and strengthen the suitability of this model.