IMMUNOPATHOLOGY OF MYCOBACTERIUM INTRACELLULARE IN AIDS

最令人苦恼和潜在严重的健康问题之一 最近在美国人的生活中发现的是获得性免疫缺陷 综合症(艾滋病)。在最初的不到3年的时间里 认识到，许多患者都被发现患有这种综合征。这个 令人痛苦和严峻的事实，疾病的严重性和非常高 死亡率促使几个相关机构和个人发起了一项 与这种疾病进行严肃的斗争。艾滋病患者变得越来越多 容易感染威胁生命的感染，由几个机会主义者引起 这样做的微生物最多只能引起良性感染过程 在普通人身上。这一应用涉及到免疫病理学 对其中一种，胞内分枝杆菌的研究。与另一个不同 机会主义微生物，胞内分枝杆菌可以引起一种难治性， 在非艾滋病患者中也存在播散型疾病。紧随其后的是 早些时候从我们的实验室获得的线索，米色的适用性 C57B1/6小鼠突变株作为艾滋病实验动物模型的可能性 将对感染细胞内支原体的患者进行调查。其他 米色小鼠的属性，除了它们增加的证据 对这种感染的易感性，是缺乏自然杀手(NK) 细胞，以及增加的抑制细胞--这是普遍存在的特征 在艾滋病患者身上。这项研究的一个重要方面是评估 艾滋病人常用的免疫抑制和娱乐药物 在这个动物模型中，患者对这种微生物的致病机制的研究 根据体内细菌增殖和死亡率来判断。为了 进一步描述和加强这一模式的主张，具体而言 用免疫评价法研究寄主的免疫调节作用 前列腺素(PGE2)及其合成及其对血管紧张素转换酶的影响 白介素2(IL-2)和干扰素(IFN)。中国的防护性研究 使用吲哚美辛(或前列腺素合成抑制剂)、IL-2和 干扰素还将补充这些药物的临床研究结果， 在这个国家已经在进行了。最后，针对具体的实验 在表征和量化T子集(T辅助(Th)和T 小鼠的抑制性(TS)细胞群和TH：TS细胞比率 模型，感染胞内分枝杆菌将增加进一步的洞察力 探讨此综合征的病理生物学，并加强其适合性。 这个型号。

One of the most distressing and potentially serious health problems recently discovered in American life is the Acquired Immune Deficiency Syndrome (AIDS). In the short span of less than 3 years after the initial recognition, many patients have been discovered to have this syndrome. The agonizing and grim facts of the seriousness of the disease and very high mortality prompted several concerned agencies and individuals to launch a serious battle against this malady. AIDS patients become increasingly susceptible to life threatening infections, caused by several opportunistic microorganisms, which do, at the most cause only benign infection processes in normal individuals. This application concerns the immunopathologic studies with one of these, M. intracellulare. Unlike the other opportunistic microorganisms, M. intracellulare can cause a refractory, disseminated type of disease in non-AIDS patients as well. Following the earlier leads obtained from our laboratories, the suitability of the Beige mutants of C57B1/6 mice as a possible experimental animal model for AIDs patients with M. intracellulare infections will be investigated. Other attributes of the Beige mice, besides the evidence of their increased susceptibility to this infection, are the absence of natural killer (NK) cells, and increased suppressor cells - features established to be common in AIDS patients. An important aspect in this study is to assess the role of immunosuppressive and recreational drugs, commonly used by the AIDS patients, on the pathogenesis of this organism in this animal model, as judged by in vivo bacterial multiplication and mortality. In order to further characterize and strengthen the claims of this model, specific investigations on the immunomodulation of the host by way of assessment of prostaglandin (PGE2) and synthesis with its consequent influence on Interleukin-2 (IL-2) and gamma interferon (IFN). Protective studies in animals using indomethacin (inhibitor or prostaglandin synthesis), IL-2 and IFN will also complement the results of clinical studies with these agents, already in progress in this country. Finally, specific experiments aimed at characterization and quantitation of the T subset (T helper (TH) and T suppressor (TS) cell) populations and the TH:TS cell ratios in this mouse model, following infection with M. intracellulare will add further insight into the pathobiology of this syndrome and strengthen the suitability of this model.