EXPRESSION OF PAPOVAVIRUS TUMOR ANTIGENS
EXPRESSION OF PAPOVAVIRUS TUMOR ANTIGENS
批准号:
3130666
负责人:
Robert Tse Nan Tjian
金额:
$7.37万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-04-01 至 1987-03-31
中文摘要
对更方便、更通用的克隆载体的需求
外源基因在真核细胞中的表达促使我们发展
一种病毒载体系统,可以容纳和表达大量的
外源基因序列。我们的系统旨在允许特定的选择
高水平表达外源基因产物的重组病毒
在病毒转录启动子的控制下。在过去的三年里
多年来,我们已经制定了许多策略来表达高水平
在哺乳动物病毒载体系统中获得SV40肿瘤抗原。我们成功了
通过构建SV40A基因获得超量表达
A基因在转录下的重组病毒
控制腺病毒的主要晚期启动子。然而,我们发现,
T基因的高效翻译似乎需要特定的引导者
与T抗原基因5‘端连接的序列。这一发现
让我们假设在我们东道主中有效地启动翻译
载体系统需要特定的顺式作用序列元件
常见于腺病毒晚期mRNAs的5‘末端。在此,我们建议
识别最近前导序列中的翻译控制信号
并探讨病毒mRNAs与功能之间的关系
三方前导序列与mRNAs的选择性翻译
病毒感染的宿主细胞。我们打算进一步探索我们的能力
通过同源基因定位外源DNA在腺病毒基因组中
重组。除了构建和分离重组
病毒,我们还将应用一系列技术来表征
表达不同的基因的基因组、转录本和蛋白质结构
腺病毒重组体。最后,我们建议将该表达式推广到
通过构建包含SV40辅助分子的克隆载体来构建
功能基因与其他外源基因的融合以选择
其他基因的表达。特别是,我们计划建造一系列
表达多发性肿瘤抗原的重组载体的设计
病毒。我们对多发性肿瘤T抗原生化特性的兴趣
源于研究表明,尽管多瘤A基因编码
一种在结构和功能上与SV40T相似的多肽
抗原的排列方式有很大差异
影响T抗原介导转录的调控序列
在多发性瘤和SV40中的病毒DNA合成。
英文摘要
The need for more convenient and versatile cloning vectors for the
expression of foreign genes in eukaryotic cells has prompted us to develop
a viral vector system that can accomodate and express a large range of
foreign gene sequences. Our system is designed to allow specific selection
of recombinant viruses that express high levels of foreign gene products
under the control of viral transcription promoters. In the past three
years, we have developed a number of strategies for expressing high levels
of the SV40 tumor antigen in a mammalian viral vector system. We succeeded
in obtaining overproduction of the SV40 A gene mRNA by constructing
recombinant viruses in which the A gene was under the transcriptional
control of the adenovirus major late promoter. However, we discovered that
efficient translation of T mRNA appeared to require specific leader
sequences to be attached to the 5' end of the T antigen mRNA. This finding
let us to hypothesize that efficient initiation of translation in our host
vector system requires specific cis-acting sequence elements that are
commonly found at the 5' ends of adenovirus late mRNAs. Here we propose to
identify the translational control signals in the leader sequences of late
viral mRNAs and to explore the relationship between the function of the
tripartite leader sequence and the selective translation of mRNAs in
virus-infected host cells. We intend to further explore our ability to
position foreign DNA within the adnovirus genome by homologous
recombination. In addition to constructing and isolating recombinant
viruses, we will also apply a battery of techniques to characterize the
genome, transcript and protein structures of the genes expressed various
adenovirus recombinants. Finally, we propose to generalize the expression
system by constructing cloning vectors that contain the SV40 helper
function gene fused to other foreign genes in order to select for
expression of other genes. In particular, we plan to construct a series of
recombinant vectors designed to express the tumor antigen of polyoma
virus. Our interest in the biochemical properties of polyoma T antigen
stems from studies which indicate that although the polyoma A gene encodes
a polypeptide that is analogous in structure and function to the SV40 T
antigen, there are significant differences between the arrangement of
regulatory sequences that influence the T antigen mediated transcription
and viral DNA synthesis in polyoma and SV40.
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会议论文
Structure and Function of Transcription Complexes
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批准号:6958362
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项目类别:
-
资助金额:$121.09万
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财政年份:2005
-
负责人:Robert Tse Nan Tjian
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依托单位:
Biochemistry and Structural Analysis of TFIID and TFIIA
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批准号:6999944
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项目类别:
-
资助金额:$19.75万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Structure and Function of Transcription Complexes
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批准号:7105080
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项目类别:
-
资助金额:$111.72万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Protein Production Facility
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批准号:6999946
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项目类别:
-
资助金额:$17.13万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Structure and Function of Transcription Complexes
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批准号:7281232
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项目类别:
-
资助金额:$113.57万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Structure and Function of Transcription Complexes
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批准号:7678444
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项目类别:
-
资助金额:$114.83万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Administrative Core
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批准号:6999947
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项目类别:
-
资助金额:$3.32万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
GORDON CONFERENCE ON MOLECULAR GENETICS
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批准号:3435172
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项目类别:
-
资助金额:$0.5万
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财政年份:1992
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负责人:Robert Tse Nan Tjian
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依托单位:
EXPRESSION OF PAPOVAVIRUS TUMOR ANTIGENS
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批准号:3130667
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项目类别:
-
资助金额:$7.27万
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财政年份:1984
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负责人:Robert Tse Nan Tjian
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依托单位:
REGULATION OF EUKARYOTIC RIBOSOMAL RNA TRANSCRIPTION
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批准号:3282024
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项目类别:
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资助金额:$12.11万
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财政年份:1983
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负责人:Robert Tse Nan Tjian
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依托单位:
REGULATION OF EUKARYOTIC RIBOSOMAL RNA TRANSCRIPTION
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批准号:3282025
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项目类别:
-
资助金额:$9.08万
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财政年份:1983
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负责人:Robert Tse Nan Tjian
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依托单位:
AUTOREGULATION OF SV40 EARLY GENE EXPRESSION
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批准号:3172493
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项目类别:
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资助金额:$6.36万
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财政年份:1983
-
负责人:Robert Tse Nan Tjian
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依托单位:
REGULATION OF EUKARYOTIC RIBOSOMAL RNA TRANSCRIPTION
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批准号:3282026
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项目类别:
-
资助金额:$9.65万
-
财政年份:1983
-
负责人:Robert Tse Nan Tjian
-
依托单位:
AUTOREGULATION OF SV40 EARLY GENE EXPRESSION
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批准号:3172494
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项目类别:
-
资助金额:$6.45万
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财政年份:1983
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 TUMOR ANTIGEN
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批准号:6350019
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项目类别:
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资助金额:$47.17万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 TUMOR ANTIGEN
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批准号:2904318
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项目类别:
-
资助金额:$45.83万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 TUMOR ANTIGEN
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批准号:6628219
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项目类别:
-
资助金额:$56.05万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 TUMOR ANTIGEN
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批准号:3482021
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项目类别:
-
资助金额:$37.73万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 Tumor Antigen
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批准号:7012291
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项目类别:
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资助金额:$50.81万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 Tumor Antigen
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批准号:7175304
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项目类别:
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资助金额:$55.38万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位: