EXPRESSION OF PAPOVAVIRUS TUMOR ANTIGENS
EXPRESSION OF PAPOVAVIRUS TUMOR ANTIGENS
批准号:
3130666
负责人:
Robert Tse Nan Tjian
金额:
$7.37万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-04-01 至 1987-03-31
中文摘要
需要更方便和通用的克隆载体
英文摘要
The need for more convenient and versatile cloning vectors for the
expression of foreign genes in eukaryotic cells has prompted us to develop
a viral vector system that can accomodate and express a large range of
foreign gene sequences. Our system is designed to allow specific selection
of recombinant viruses that express high levels of foreign gene products
under the control of viral transcription promoters. In the past three
years, we have developed a number of strategies for expressing high levels
of the SV40 tumor antigen in a mammalian viral vector system. We succeeded
in obtaining overproduction of the SV40 A gene mRNA by constructing
recombinant viruses in which the A gene was under the transcriptional
control of the adenovirus major late promoter. However, we discovered that
efficient translation of T mRNA appeared to require specific leader
sequences to be attached to the 5' end of the T antigen mRNA. This finding
let us to hypothesize that efficient initiation of translation in our host
vector system requires specific cis-acting sequence elements that are
commonly found at the 5' ends of adenovirus late mRNAs. Here we propose to
identify the translational control signals in the leader sequences of late
viral mRNAs and to explore the relationship between the function of the
tripartite leader sequence and the selective translation of mRNAs in
virus-infected host cells. We intend to further explore our ability to
position foreign DNA within the adnovirus genome by homologous
recombination. In addition to constructing and isolating recombinant
viruses, we will also apply a battery of techniques to characterize the
genome, transcript and protein structures of the genes expressed various
adenovirus recombinants. Finally, we propose to generalize the expression
system by constructing cloning vectors that contain the SV40 helper
function gene fused to other foreign genes in order to select for
expression of other genes. In particular, we plan to construct a series of
recombinant vectors designed to express the tumor antigen of polyoma
virus. Our interest in the biochemical properties of polyoma T antigen
stems from studies which indicate that although the polyoma A gene encodes
a polypeptide that is analogous in structure and function to the SV40 T
antigen, there are significant differences between the arrangement of
regulatory sequences that influence the T antigen mediated transcription
and viral DNA synthesis in polyoma and SV40.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Structure and Function of Transcription Complexes
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批准号:6958362
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项目类别:
-
资助金额:$121.09万
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财政年份:2005
-
负责人:Robert Tse Nan Tjian
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依托单位:
Biochemistry and Structural Analysis of TFIID and TFIIA
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批准号:6999944
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项目类别:
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资助金额:$19.75万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Structure and Function of Transcription Complexes
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批准号:7105080
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项目类别:
-
资助金额:$111.72万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Protein Production Facility
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批准号:6999946
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项目类别:
-
资助金额:$17.13万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Structure and Function of Transcription Complexes
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批准号:7281232
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项目类别:
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资助金额:$113.57万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Structure and Function of Transcription Complexes
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批准号:7678444
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项目类别:
-
资助金额:$114.83万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
Administrative Core
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批准号:6999947
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项目类别:
-
资助金额:$3.32万
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财政年份:2005
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负责人:Robert Tse Nan Tjian
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依托单位:
GORDON CONFERENCE ON MOLECULAR GENETICS
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批准号:3435172
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项目类别:
-
资助金额:$0.5万
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财政年份:1992
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负责人:Robert Tse Nan Tjian
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依托单位:
EXPRESSION OF PAPOVAVIRUS TUMOR ANTIGENS
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批准号:3130667
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项目类别:
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资助金额:$7.27万
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财政年份:1984
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负责人:Robert Tse Nan Tjian
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依托单位:
REGULATION OF EUKARYOTIC RIBOSOMAL RNA TRANSCRIPTION
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批准号:3282024
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项目类别:
-
资助金额:$12.11万
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财政年份:1983
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负责人:Robert Tse Nan Tjian
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依托单位:
REGULATION OF EUKARYOTIC RIBOSOMAL RNA TRANSCRIPTION
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批准号:3282025
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项目类别:
-
资助金额:$9.08万
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财政年份:1983
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负责人:Robert Tse Nan Tjian
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依托单位:
AUTOREGULATION OF SV40 EARLY GENE EXPRESSION
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批准号:3172493
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项目类别:
-
资助金额:$6.36万
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财政年份:1983
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负责人:Robert Tse Nan Tjian
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依托单位:
REGULATION OF EUKARYOTIC RIBOSOMAL RNA TRANSCRIPTION
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批准号:3282026
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项目类别:
-
资助金额:$9.65万
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财政年份:1983
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负责人:Robert Tse Nan Tjian
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依托单位:
AUTOREGULATION OF SV40 EARLY GENE EXPRESSION
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批准号:3172494
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项目类别:
-
资助金额:$6.45万
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财政年份:1983
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 TUMOR ANTIGEN
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批准号:2904318
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项目类别:
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资助金额:$45.83万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 TUMOR ANTIGEN
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批准号:6350019
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项目类别:
-
资助金额:$47.17万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 TUMOR ANTIGEN
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批准号:6628219
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项目类别:
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资助金额:$56.05万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 TUMOR ANTIGEN
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批准号:3482021
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项目类别:
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资助金额:$37.73万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 Tumor Antigen
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批准号:7012291
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项目类别:
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资助金额:$50.81万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位:
SV40 Tumor Antigen
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批准号:7175304
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项目类别:
-
资助金额:$55.38万
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财政年份:1979
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负责人:Robert Tse Nan Tjian
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依托单位: