STRUCTURE AND FUNCTION OF HUMAN SECRETORY COMPONENT
STRUCTURE AND FUNCTION OF HUMAN SECRETORY COMPONENT
批准号:
3131510
负责人:
Randall M Goldblum
金额:
$9.93万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-07-01 至 1988-03-31
关键词:
affinity chromatography chemical structure function conformation enzyme linked immunosorbent assay epithelium gel electrophoresis high performance liquid chromatography immunofluorescence technique immunoglobulin A monoclonal antibody protease inhibitor proteolysis receptor mediated endocytosis secretion secretory immune system
中文摘要
分泌成分(SC)在粘膜免疫中起着核心作用
英文摘要
Secretory component (SC) plays a central role in the mucosal immune
system. It functions as an intergal membrane receptor, transcellular
transporter, and protease inhibitor for its ligand polymeric IgA. A panel
of monoclonal antibodies to human SC, characterized in our laboratories,
offers a unique opportunity to study the immunobiology of SC. Our basic
objective is to examine the structural characteristics which allow SC to
function in its multiple specialized roles. Clinical studies will seek
evidence for heterogeneity in size and structure of SC in different
secretions and from different individuals. These studies may provide
evidence for a modulatory role for SC and insight into alterations in SC
metabolism in malignant epithelial cells.
The SC from various secretions will be isolated by immunoaffinity
chromatography and certain preparations will be modified by deglycosylation
and limited and enzymatic proteolysis. Initially SDS polyacrylamide gel
electrophoresis, HPLC and reaction with monoclonal antibodies will be used
to characterize the size and epitopic structure of the products.
Subsequently, amino acid and carbohydrate composition and amino acid
sequence analysis will be determined for certain SC fragments. The in
vitro function of the SC preparations will be assessed by evaluating
polymeric IgA binding and inhibition of IgA proteolysis. Intracellular
events will be examined using a colon carcinoma derived cell line. The
intracellular pathway of SC will be followed by immunocytochemistry using
monoclonal antibodies which distinguish the various physical forms of SC.
Glycosylation will be inhibited with tunicamycin and attention focused on
the role of carbohydrate in SC cellular function.
Human IgG antibodies to SC will be used to probe the in vivo conformation
of the receptor form of SC by comparing the spectrum of antibodies in the
serum and secretions of individuals. A modulatory role for these
antibodies will be tested in an in vitro model of SC function.
Deliniation of the normal structure and function of SC will enhance our
understanding of mucosal immunity and its control. Therapeutic
applications of this knowledge may include the development of methods for
targeting active or passive immunity or other therapeutic agents to the
site of mucosal infections. The proposed studies may also elucidate
alterations in SC metabolism in malignant epithelial cells which may allow
direction of cytotoxic or chemotherapeutic agent selectively to these
tumors.
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Prevention of Allergic Rhinitis with Topical Immunomodulating Antibodies
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批准号:8201881
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项目类别:
-
资助金额:$30.0万
-
财政年份:2011
-
负责人:Randall M Goldblum
-
依托单位:
Phylogenetic Approach to Plant Allergy Vaccines
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批准号:7760239
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项目类别:
-
资助金额:$29.49万
-
财政年份:2009
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负责人:Randall M Goldblum
-
依托单位:
Phylogenetic Approach to Plant Allergy Vaccines
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批准号:6861095
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项目类别:
-
资助金额:$26.43万
-
财政年份:2004
-
负责人:Randall M Goldblum
-
依托单位:
Phylogenetic Approach to Plant Allergy Vaccines
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批准号:7029666
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项目类别:
-
资助金额:$29.24万
-
财政年份:2004
-
负责人:Randall M Goldblum
-
依托单位:
Phylogenetic Approach to Plant Allergy Vaccines
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批准号:7225584
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项目类别:
-
资助金额:$29.49万
-
财政年份:2004
-
负责人:Randall M Goldblum
-
依托单位:
Phylogenetic Approach to Plant Allergy Vaccines
-
批准号:6723560
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项目类别:
-
资助金额:$26.17万
-
财政年份:2004
-
负责人:Randall M Goldblum
-
依托单位:
CORE--LABORATORY
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批准号:6564691
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项目类别:
-
资助金额:$19.74万
-
财政年份:2001
-
负责人:Randall M Goldblum
-
依托单位:
CORE--LABORATORY
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批准号:6439488
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项目类别:
-
资助金额:$19.74万
-
财政年份:2000
-
负责人:Randall M Goldblum
-
依托单位:
CORE--LABORATORY
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批准号:6301969
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项目类别:
-
资助金额:$26.41万
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财政年份:1999
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负责人:Randall M Goldblum
-
依托单位:
CORE--LABORATORY
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批准号:6108576
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项目类别:
-
资助金额:$26.41万
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财政年份:1998
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负责人:Randall M Goldblum
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依托单位:
CORE--LABORATORY
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批准号:6272186
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项目类别:
-
资助金额:$26.11万
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财政年份:1998
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负责人:Randall M Goldblum
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依托单位:
IGA CELLULAR RECEPTOR INTERACTIONS IN IGA NEPHROPATHY
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批准号:2150049
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项目类别:
-
资助金额:$26.47万
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财政年份:1994
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负责人:Randall M Goldblum
-
依托单位:
IGA CELLULAR RECEPTOR INTERACTIONS IN IGA NEPHROPATHY
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批准号:2150048
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项目类别:
-
资助金额:$23.77万
-
财政年份:1994
-
负责人:Randall M Goldblum
-
依托单位:
IGA CELLULAR RECEPTOR INTERACTIONS IN IGA NEPHROPATHY
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批准号:2518452
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项目类别:
-
资助金额:$27.26万
-
财政年份:1994
-
负责人:Randall M Goldblum
-
依托单位:
IGA CELLULAR RECEPTOR INTERACTIONS IN IGA NEPHROPATHY
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批准号:2150050
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项目类别:
-
资助金额:$26.22万
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财政年份:1994
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负责人:Randall M Goldblum
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依托单位:
DEVELOP METHODS OF ANALYSIS OF HUMAN COLOSTRUM AND MILK
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批准号:3652198
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项目类别:
-
资助金额:$0.0万
-
财政年份:1986
-
负责人:Randall M Goldblum
-
依托单位:
DEVEL OF METHODS OF ANALYSIS OF HUMAN COLOSTRUM AND MILK
-
批准号:3652196
-
项目类别:
-
资助金额:$14.79万
-
财政年份:1986
-
负责人:Randall M Goldblum
-
依托单位:
DEVEL OF METHODS OF ANALYSIS OF HUMAN COLOSTRUM AND MILK
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批准号:3652194
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项目类别:
-
资助金额:$11.22万
-
财政年份:1986
-
负责人:Randall M Goldblum
-
依托单位:
METHODS OF ANALYSIS--HUMAN COLOSTRUM AND MILK
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批准号:3652197
-
项目类别:
-
资助金额:$15.26万
-
财政年份:1986
-
负责人:Randall M Goldblum
-
依托单位:
DEVEL OF METHODS OF ANALYSIS OF HUMAN COLOSTRUM AND MILK
-
批准号:3652195
-
项目类别:
-
资助金额:$0.0万
-
财政年份:1986
-
负责人:Randall M Goldblum
-
依托单位:
海外基金