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MUTATIONAL ANALYSIS OF SEXUAL DEVELOPMENT IN PHYCOMYCES

藻类性发育的突变分析

基本信息

批准号：
3132200
负责人：
RICHARD P SUTTER
金额：
$ 5.46万
依托单位：
WEST VIRGINIA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-12-01 至 1989-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3132200
关键词：
chemical synthesis fungal genetics gene complementation growth /development high performance liquid chromatography hormone regulation /control mechanism microorganism culture mutant pheromone

项目摘要

The ultimate goal of the project is to understand in molecular detail the mechanisms regulating sexual development in Phycomyces. This system provides a fascinating example of mutual signaling between two types of cells. Such signaling may be the root of the organizing principle in mammals. The system also provides an example of elementary differentiation. Pheromones initiate sexual development by stimulating zygophore (sex cell)-development, carotenogenesis, their own biosynthesis, and possibly all other facets of sexual development. The pheromones made by one sex are active only in cultures of the opposite sex. The structures of 9 sex pheromones active in Phycomyces are known; their mechanisms of action are unknown. The mutational analysis outlined in this proposal provides a basis for obtaining mechanistic answers for some (if not all) aspects of pheromone function. Work centers upon screening for 120 mutants blocked in early sexual development and then identifying their defects behaviorally, biochemically, and genetically. This specific project: 1) will determine whether the sex pheromones or their metabolites, the trisporic acids, actually regulate zygophore-development, 2) will indicate the (minimum) number of events occurring between pheromone-receptor and zygophore-development, and 3) will identify the (early) sex-specific events. More importantly, this project will provide mutants which can be used subsequently in the analysis of: growth arrest, the regulation of pheromone biosynthesis, the regulation of zygophore-development, and (possibly) the molecular basis of sexuality. Furthermore, the overall project may reveal a novel regulatory mechanism, unrecognized to date, which could be present in all organisms. The more that is known about regulatory mechanisms at any level of biological organization, the better the prospects of discovering how abnormal mammalian cells function and of discovering how to bring them under control.

该项目的最终目标是了解分子细节，调节性发育的机制。该系统提供了一个有趣的例子，说明两种类型的细胞这样的信号可能是组织原则的根源，哺乳动物该系统还提供了一个基本的例子，分化信息素通过刺激性发育来启动性发育生殖细胞-发育，胡萝卜素生成，自身生物合成，可能还有性发育的其他方面制造的信息素只有在异性文化中才活跃。的结构已知9种性信息素中的1种，它们的作用机制行动不明。这份提案中概述的突变分析为获得某些（如果不是全部）机械答案提供了基础信息素功能的各个方面。工作重点是筛选120个突变体在早期性发育中受阻，然后发现他们的缺陷行为上生化上和基因上具体项目：1）将决定性信息素或其代谢物，三孢子酸，实际上调节孢子柄的发育，2）将表明信息素受体之间发生的事件（最小）数量， 3）将确定（早期）性别特异性事件更重要的是，这个项目将提供突变体，随后用于分析：生长停滞，信息素的生物合成，调节植物生长发育，（可能）性的分子基础。此外，总体该项目可能揭示了一种新的监管机制，迄今为止尚未认识到，可能存在于所有生物体中我们知道的越多在任何生物组织水平上的调节机制，发现异常哺乳动物细胞功能的前景，探索如何控制它们