DIFFERENTIAL ACTIVATION REQUIREMENTS OF CLONED T CELLS

克隆 T 细胞的差异激活要求

• 批准号: 3137647
• 负责人: Rosemarie H DeKruyff
• 金额: $ 11.33万
• 依托单位: LUCILE SALTER PACKARD CHILDREN'S HOSP
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3137647
• 关键词: B lymphocyte; CD4 molecule; T lymphocyte; antibody formation; antigen presenting cell; cell cell interaction; cell differentiation; cell population study; clone cells; enzyme linked immunosorbent assay; helper T lymphocyte; immunoregulation; interleukin 2; interleukin 4; interleukin 5; interleukin 6; laboratory mouse; leukocyte activation /transformation

The long term goal of this project is to gain further insight into the mechanisms governing the activation of CD4+ T cell subsets, and the mechanisms governing T-B cell interactions which result in the induction of antibody synthesis.' The specific aims of this project are to: 1. precisely define the conditions which lead to the activation and expansion of Th1 versus Th2 T cells. 2. examine the characteristics of Th1 and Th2 clones which are capable of inducing antibody synthesis, and dissect the mechanism by which they perform this function. 3. examine the role of Th1 and Th2 "nonhelper" clones in the induction of antibody synthesis. To perform this project, our lab has generated a large panel of nominal antigen specific Th1 and Th2 clones, which are heterogeneous with regard to their profile of lymphokine production and with regard to their ability to provide cognate help in the induction of antibody synthesis. In addition, we have established accurate and sensitive ELISA assays for IgG, IgG subclasses, IgA, and IgE, and have enlisted the support of other investigators to help us apply molecular biology techniques in the analysis of our clones. With our ability to purify B cell and accessory cell populations, we have available powerful tools to analyze T cell activation and function at the clonal level. Since isotype specific responses require the presence of distinct lymphokines, and since responses to different types of pathogens require distinct functions of T cells, the division of CD4+ T cells into subsets may be of fundamental importance in the regulation of immune responses. Thus understanding of the requirements for selective activation of one type of CD4+ T cell subset over another, and of the role of each subset in the induction of antibody synthesis is crucial for future development of strategies to favorably manipulate the immune system in multiple disease states.

该项目的长期目标是进一步深入了解 调节CD4+T细胞亚群激活的机制，以及 T-B细胞相互作用导致诱导的机制 抗体合成的技术。 这个项目的具体目标是：1.准确定义 导致Th1/Th2 T细胞活化和扩增的条件 细胞。2.检查Th1和Th2克隆的特征 能够诱导抗体合成，并通过以下方式剖析其机制 它们执行这一功能。3.研究Th1和Th2的作用 “非辅助性”克隆在诱导抗体合成中的作用。 为了执行这个项目，我们的实验室生成了一个很大的标称面板 抗原特异性Th1和Th2克隆，它们在以下方面是不同的 关于淋巴因子产生的概况以及关于他们的 在抗体合成诱导中提供同源帮助的能力。 此外，我们还建立了准确和灵敏的ELISA检测方法 Ig G、Ig G亚类、Ig A和Ig E，并已争取到其他 研究人员帮助我们将分子生物学技术应用于 分析我们的克隆人。我们有能力提纯B细胞和辅助细胞 细胞群体，我们有可用的强大工具来分析T细胞 在克隆水平上的激活和功能。 由于同种类型特定的响应需要存在不同的 淋巴因子，而且由于对不同类型的病原体的反应需要 T细胞的不同功能，将CD4+T细胞分裂成亚群 可能在免疫反应的调节中起着至关重要的作用。 从而理解选择性激活One的要求 CD4+T细胞亚群相对于另一个亚群的类型，以及每个亚群的作用 在诱导抗体合成方面对未来的发展至关重要 一系列有利地操控免疫系统的策略 疾病状态。