FACTORS INFLUENCING SURVIVAL OF VASCULARIZED BONE GRAFTS

影响血管化骨移植存活的因素

• 批准号: 3155353
• 负责人: ANDREW J WEILAND
• 金额: $ 16.79万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3155353
• 关键词: blood flow measurement; blood vessel transplantation; bone imaging /visualization /scanning; bone transplantation; cyclosporines; disease /disorder model; electron microscopy; epiphysis; genetic strain; histochemistry /cytochemistry; homologous transplantation; immunosuppressive; microangiography; muscle transplantation; musculoskeletal circulation; osteomyelitis; radiotracer; skin transplantation

This proposal seeks to: 1. Evaluate bone allograft transplantation in a genetically defined rat model in order to systemically gather fundamental immunologic information concerning transplantation of vascularized bone allografts. Protocols have been designed to identify manifestations and, perhaps, the focus of the rejection process in the graft and to determine graft-host factors that affect allograft survival. Light microscopy, electron microscopy, and enzyme histochemistry will be employed to evaluate the rejection process; attempts will be made to alter graft antigenicity and evaluate the affect of cyclosporin-A immunosuppression on graft survival with the long-term objective of developing the used of allogeneic tissue in extremity reconstruction. 2. Evaluate the efficacy of free vascularized muscle flap coverage in the treatment of chronic osteomyelitis in a canine model with the objective of clarifying the role of this technique in clinical reconstructive surgery. 3. Evaluate the growth in orthotopically and heterotopically transplanted epiphyseal plates in a primate model in order to attempt to define the possible clinical role of vascularized epiphyseal transplantation in reconstructive surgery for the treatment of radial club hand, tumors involving epithyses of long bones, and the transferring of digits from the foot to the hand for congenital or traumatic conditions in children. 4. Evaluate the feasibility of molded vascularized osteoneogenesis using a titanium chamber. The possibility of producing bone with a predetermined size and shape as well as a defined vascular nutrient stalk would greatly simplify reconstruction of segmental bone defects. The results of this technique will be compared to conventional reconstructive procedures.

这项建议旨在： 1.对大鼠同种异体骨移植进行评估 模型，以便系统地收集基础免疫学信息 关于带血管的同种异体骨移植。协议有 被设计用来识别表现形式，也许还有 移植物中的排斥反应过程，并确定移植物宿主因子 影响同种异体移植物存活。光学显微镜、电子显微镜和 用酶组织化学方法评价排斥反应过程； 将尝试改变移植物的抗原性并评估其影响 环孢素-A免疫抑制对移植物长期存活的影响 目的发展同种异体组织在四肢中的应用 重建。 2.评价带血管蒂肌瓣游离移植的疗效。 慢性骨髓炎犬模型的治疗 阐明该技术在临床重建外科中的作用。 3.原位移植和异位移植的生长发育评价 在灵长类动物模型中的骨骺板，以试图定义 带血管蒂骨盆移植在临床中的可能作用 重建手术治疗桡骨杆状手肿瘤 累及长骨上皮，以及从长骨上皮起的手指移位 儿童先天性或创伤性疾病的足部到手部。 4.评估模塑血管化成骨的可行性。 钛室。生产骨骼的可能性与预先确定的 大小和形状以及确定的血管营养茎将大大 简化节段性骨缺损的重建。这样做的结果 这项技术将与传统的重建程序进行比较。