FACTORS INFLUENCING SURVIVAL OF VASCULARIZED BONE GRAFTS

这项建议旨在： 1.评估遗传定义大鼠的同种异体骨移植 为了系统地收集基本的免疫信息， 关于血管化骨同种异体移植物的移植。 协议已经 被设计来确定表现形式，也许， 移植物中的排斥过程，并确定 影响同种异体移植物存活。 光学显微镜，电子显微镜， 将采用酶组织化学来评价排斥过程; 将尝试改变移植物抗原性并评估其影响 环孢素A免疫抑制对移植物存活的长期影响 目的发展同种异体组织在肢体的应用 重建 2.带血管蒂肌瓣游离移植修复下肢深静脉曲张的疗效评价 治疗犬模型中的慢性骨髓炎，目的是 阐明该技术在临床重建手术中的作用。 3.评估原位和异位移植的生长情况 在灵长类动物模型中的骺板，以尝试定义 带血管蒂骨骺移植在骨骺移植中的临床应用 桡骨俱乐部手的重建手术，肿瘤 包括长骨的上皮细胞，以及手指从 从脚到手，用于儿童的先天性或创伤性疾病。 4.评价使用血管化骨再生技术进行塑形的可行性 钛合金室 利用预定的骨密度来制造骨的可能性 大小和形状以及确定的维管营养茎将大大 节段性骨缺损简化重建。 的结果 技术将与传统的重建程序进行比较。

This proposal seeks to: 1. Evaluate bone allograft transplantation in a genetically defined rat model in order to systemically gather fundamental immunologic information concerning transplantation of vascularized bone allografts. Protocols have been designed to identify manifestations and, perhaps, the focus of the rejection process in the graft and to determine graft-host factors that affect allograft survival. Light microscopy, electron microscopy, and enzyme histochemistry will be employed to evaluate the rejection process; attempts will be made to alter graft antigenicity and evaluate the affect of cyclosporin-A immunosuppression on graft survival with the long-term objective of developing the used of allogeneic tissue in extremity reconstruction. 2. Evaluate the efficacy of free vascularized muscle flap coverage in the treatment of chronic osteomyelitis in a canine model with the objective of clarifying the role of this technique in clinical reconstructive surgery. 3. Evaluate the growth in orthotopically and heterotopically transplanted epiphyseal plates in a primate model in order to attempt to define the possible clinical role of vascularized epiphyseal transplantation in reconstructive surgery for the treatment of radial club hand, tumors involving epithyses of long bones, and the transferring of digits from the foot to the hand for congenital or traumatic conditions in children. 4. Evaluate the feasibility of molded vascularized osteoneogenesis using a titanium chamber. The possibility of producing bone with a predetermined size and shape as well as a defined vascular nutrient stalk would greatly simplify reconstruction of segmental bone defects. The results of this technique will be compared to conventional reconstructive procedures.