COLLABORATIVE PROJECT: MAGIC - A multi-tiered approach to generating increased carbon dioxide for photosynthesis

合作项目:MAGIC - 为光合作用产生更多二氧化碳的多层方法

基本信息

  • 批准号:
    BB/I02450X/1
  • 负责人:
  • 金额:
    $ 25.36万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2011
  • 资助国家:
    英国
  • 起止时间:
    2011 至 无数据
  • 项目状态:
    已结题

项目摘要

Photosynthesis is at the core of virtually every aspect of society, from food production to industrial construction. Terrestrial photosynthesis is intimately connected with our use of other natural resources, and it exerts major controls on the water, mineral and carbon cycles of the world. For example, plant transpiration is thought to have contributed to recent changes in fresh-water availability associated with the global rise in CO2, and it is at the centre of a crisis in water availability expected over the next 20-30 years. Over this same period it is estimated that a 50% increase in global food production will be required to keep pace with the increase in human population. Crop yields have matched population growth until recently, but the gains from cereal cultivars bred in the Green Revolution were realised in full a decade ago. Thus it is vital that routes to further improvements in photosynthetic efficiency are sought now. In most species, CO2 is fixed by Ribulose Bisphosphate Carboxylase/Oxygenase (RuBisCO) in the Calvin-Benson cycle to generate a three-carbon compound. RuBisCO is remarkably poor in its substrate selectivity and promiscuously fixes both CO2 and O2, a fact that makes RuBisCO arguably the most inefficient step in photosynthesis. One way of reducing O2 use by RuBisCO is to raise the partial pressure of CO2 (pCO2). So-called carbon concentrating mechanisms (CCMs) have evolved multiple times in nature, albeit not as a feature of most common crop species. Thus, comparisons suggest roughly a 50% increase in overall yield might be realised if O2 use by RuBisCO were bypassed in crops. Significant resources have gone into engineering RuBisCO for increased CO2 selectivity and introducing a single-celled version of C4 photosynthesis in rice, but these approaches have yet to see a step change in photosynthetic efficiency. One new set of strategies yet to be explored is to co-opt light-driven pumps, anion exchange transport and substrate channelling to supply CO2 to RuBisCO. To date none of these processes is known to facilitate photosynthesis, although all three occur naturally and have been employed synthetically in biology. It is our goal to develop the equivalent of a 'two-stage pump': placing in series (1) a transport mechanism to concentrate HCO3- in the chloroplast powered by the light-driven ion pump halorhodopsin (hR) from the archeon Halobacterium halobium, and (2) substrate channelling within the chloroplast using one or more molecular 'building blocks' from Clostridium or cyanobacteria to carry HCO3- or a four-carbon intermediate to RuBisCO. This two-stage strategy is expected to maximise CCM gain driven independently with light energy absorbed by hR, and it has the added potential for engineering hR to tap the unused asset of light beyond the photosynthetic spectrum. Furthermore, an overarching feature of this approach is in its modular nature: it will be possible to develop each stage of the two-stage pump in parallel, and to assess its functionality separately at molecular, cellular and whole-organismal levels, combining the components thereafter for final validation. This modular approach ensures the maximum efficiency and speed in realising our goal within the three-year period.
光合作用几乎是社会各个方面的核心,从食品生产到工业建设。陆地光合作用与我们对其他自然资源的使用密切相关,它对世界的水,矿物和碳循环施加重大控制。例如,植物蒸腾作用被认为是造成最近与全球二氧化碳上升有关的淡水供应变化的原因,它是预计在未来20-30年内水供应危机的核心。据估计,在同一时期,全球粮食产量需要增加50%才能跟上人口增长的步伐。直到最近,作物产量一直与人口增长相匹配,但在绿色革命中培育的谷物品种的收益在十年前就已经实现了。因此,现在寻求进一步提高光合效率的途径是至关重要的。在大多数物种中,CO2通过Calvin-Benson循环中的核酮糖二磷酸羧化酶/加氧酶(RuBisCO)固定,以产生三碳化合物。RuBisCO的底物选择性非常差,并且混杂地固定CO2和O2,这一事实使得RuBisCO可以说是光合作用中最低效的步骤。减少RuBisCO使用O2的一种方法是提高CO2分压(pCO 2)。所谓的碳浓缩机制(CCM)在自然界中已经进化了多次,尽管不是大多数常见作物物种的特征。因此,比较表明,如果在作物中绕过RuBisCO的O2使用,可能会实现总体产量增加约50%。大量的资源已经投入到工程RuBisCO中,以提高CO2的选择性,并在水稻中引入单细胞版本的C4光合作用,但这些方法还没有看到光合效率的阶跃变化。一套有待探索的新策略是利用光驱动泵、阴离子交换运输和底物通道向RuBisCO提供CO2。到目前为止,这些过程中没有一个是已知的,以促进光合作用,虽然所有三个自然发生,并已在生物学合成。我们的目标是开发相当于“两级泵”的产品:串联设置(1)由来自Archeon Halobacterium halobium的光驱动离子泵Halorhodopsin(hR)提供动力的在叶绿体中浓缩HCO 3-的运输机制,和(2)使用来自梭菌属或蓝细菌的一种或多种分子“构件”在叶绿体内进行底物通道化,以携带HCO 3-或四-碳中间体RuBisCO。这种两阶段策略预计将最大限度地提高CCM增益,独立驱动hR吸收的光能,并且它具有工程化hR的额外潜力,以利用光合光谱之外的未使用的光资产。此外,这种方法的首要特征是其模块化性质:可以并行开发两级泵的每个阶段,并在分子,细胞和整个生物体水平上分别评估其功能,然后将组件组合起来进行最终验证。这种模块化方法确保了在三年内实现我们目标的最大效率和速度。

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Spatial photosynthesis modelling sets guidelines to constructing a viable single-cell cytoplasm-to-stroma C 4 cycle
空间光合作用模型为构建可行的单细胞细胞质到基质 C 4 循环提供了指导
  • DOI:
    10.1101/274845
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Juric I
  • 通讯作者:
    Juric I
Computational modelling predicts substantial carbon assimilation gains for C3 plants with a single-celled C4 biochemical pump.
计算模型预测使用单细胞 C4 生化泵的 C3 植物可大幅增加碳同化。
  • DOI:
    10.1371/journal.pcbi.1007373
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    4.3
  • 作者:
    Juric I
  • 通讯作者:
    Juric I
Size matters for single-cell C4 photosynthesis in Bienertia.
  • DOI:
    10.1093/jxb/erw374
  • 发表时间:
    2017-01
  • 期刊:
  • 影响因子:
    6.9
  • 作者:
    Jurić I;González-Pérez V;Hibberd JM;Edwards G;Burroughs NJ
  • 通讯作者:
    Burroughs NJ
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Nigel Burroughs其他文献

Kinesin Binding Expands and Stabilises the GDP-Microtubule Lattice
  • DOI:
    10.1016/j.bpj.2017.11.2769
  • 发表时间:
    2018-02-02
  • 期刊:
  • 影响因子:
  • 作者:
    Daniel Peet;Nigel Burroughs;Robert A. Cross
  • 通讯作者:
    Robert A. Cross
Relating the approaches to quantised algebras and quantum groups

Nigel Burroughs的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Nigel Burroughs', 18)}}的其他基金

14-PSIL MAGIC: a multi-tiered approach to gaining increased carbon
14-PSIL MAGIC:增加碳的多层方法
  • 批准号:
    BB/M011291/1
  • 财政年份:
    2015
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Research Grant
Genome wide identification of priming sites for Okazaki fragments
冈崎片段引发位点的全基因组鉴定
  • 批准号:
    BB/K018272/1
  • 财政年份:
    2013
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Research Grant
Quantification modelling and analysis of molecular dynamics patterning and signalling in the NK synapse.
NK 突触分子动力学模式和信号传导的量化建模和分析。
  • 批准号:
    BB/D011663/1
  • 财政年份:
    2006
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Research Grant

相似海外基金

HSI Pilot Project: Institutionalizing a Teaching and Learning Excellence Community of Practice focused on First-Year Student Success in STEM
HSI 试点项目:将卓越教学和学习实践社区制度化,重点关注一年级学生在 STEM 方面的成功
  • 批准号:
    2345247
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Standard Grant
HSI Implementation and Evaluation Project: Leveraging Social Psychology Interventions to Promote First Year STEM Persistence
HSI 实施和评估项目:利用社会心理学干预措施促进第一年 STEM 的坚持
  • 批准号:
    2345273
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Standard Grant
HSI Implementation and Evaluation Project: Green Chemistry: Advancing Equity, Relevance, and Environmental Justice
HSI 实施和评估项目:绿色化学:促进公平、相关性和环境正义
  • 批准号:
    2345355
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Continuing Grant
HSI Implementation and Evaluation Project: Blending Socioeconomic-Inclusive Design into Undergraduate Computing Curricula to Build a Larger Computing Workforce
HSI 实施和评估项目:将社会经济包容性设计融入本科计算机课程,以建立更大规模的计算机队伍
  • 批准号:
    2345334
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Continuing Grant
Project Incubation: Training Undergraduates in Collaborative Research Ethics
项目孵化:培养本科生合作研究伦理
  • 批准号:
    2316154
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Standard Grant
NESP MaC Project 4.5– Developing an Integrated Pest Management Framework for Feral Pigs in Coastal Environments 2024-2026 (NAILSMA)
NESP MaC 项目 4.5 — 为 2024-2026 年沿海环境中的野猪制定综合害虫管理框架 (NAILSMA)
  • 批准号:
    global : ba1e00f0-9953-4c17-b990-ba7aed84ce07
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
Women’s Careers in STEM: a Multi-method Project from an Indigenous Perspective
STEM 领域的女性职业:从原住民角度看的多方法项目
  • 批准号:
    24K16421
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
e-health tools to promote Equality in Quality of Life for childhood to young adulthood cancer patients, survivors and their families - a PanEuropean project supported by PanCare and Harmonic consortia
电子医疗工具可促进儿童到成年癌症患者、幸存者及其家人的生活质量平等 - 这是由 PanCare 和 Harmonic 联盟支持的 PanEuropean 项目
  • 批准号:
    10098114
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    EU-Funded
Net Zero Rail Product Commercialisation Project
净零轨产品商业化项目
  • 批准号:
    10098199
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Collaborative R&D
Priceworx Ultimate+: A world-first AI-driven material cost forecaster for construction project management.
Priceworx Ultimate:世界上第一个用于建筑项目管理的人工智能驱动的材料成本预测器。
  • 批准号:
    10099966
  • 财政年份:
    2024
  • 资助金额:
    $ 25.36万
  • 项目类别:
    Collaborative R&D
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了