A functional genomics toolkit for the wheat pathogen Mycosphaerella graminicola

小麦病原菌禾本科球腔菌的功能基因组学工具包

基本信息

  • 批准号:
    BB/I025956/1
  • 负责人:
  • 金额:
    $ 110.48万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2012
  • 资助国家:
    英国
  • 起止时间:
    2012 至 无数据
  • 项目状态:
    已结题

项目摘要

Wheat is the principle food crop in Europe, including Britain where the annual value of wheat production is approximately £1.4 billion. With the expectation that planet's population will increase to over 9 billion by 2050, we urgently need to find ways to provide more food to support this growth. One of the major threats to wheat production is fungal disease. The pathogen Mycosphaerella graminicola is the major disease-causing agent of wheat in the UK and is one of the principle reasons why over £500 million of fungicides are applied to protect cereal crops in the UK alone. Despite this there are still major yield losses. Hence there is an urgent need for new durable disease control strategies, including novel fungicides. The main difficulty in developing novel fungicides is the inability to determine how new molecules work to kill the fungus. In the model fungus brewer's/baker's yeast, Saccharomyces cerevisiae, powerful tools have been generated, such as sets of yeast strains; lacking each fungal gene; that have each protein tagged with a fluorescent protein, or where each individual protein is expressed at a high level. These resources have allowed large-scale determination of how a wide range of chemicals function to alter the biology of yeast. We intend to construct a similar complete set of tools that will allow us to perform these assays, and hence determine how novel fungicides work, in the wheat pathogen M. graminicola. Specifically, we will place each of its ~10,000 genes into a mobile genetic element (plasmid) that will allow easy transfer into a large range of other plasmids (that we will also create) that can be used to inactivate, fluorescently tag and over-express each gene, both individually and as a pool. We will also construct a series of M. graminicola strains that will allow us to determine how the natural fungus invades wheat, and we will develop a laboratory model of wheat infection to achieve this. This will significantly add to the value of the current investment in sequencing the wheat genome. We will then use these resources to determine how fungicides impact on the infection process, and hence circumvent the principle impediment to the development of new control measures. To ensure that these resources are made widely available we will develop a repository for these plasmids and strains, place them in international depositories, write standard operating procedures to allow maximal exploitation and create a website to advertise and allow access to the resources. Finally, we will interact with stakeholders, both in the South-West (eg the new integrated farm platform at BBSRC North Wyke), and nationally (eg Rothamsted Research, Sainsbury Laboratory, and Syngenta) to increase the impact and dissemination of the tools generated.
小麦是欧洲的主要粮食作物,包括英国,其小麦年产量约为14亿GB。由于预计到2050年地球人口将增加到90亿以上,我们迫切需要找到方法来提供更多的食物来支持这一增长。小麦生产的主要威胁之一是真菌病。禾谷分枝杆菌是英国小麦的主要病原菌,也是仅在英国就有超过5亿GB的杀菌剂用于保护谷类作物的主要原因之一。尽管如此,产量仍有较大损失。因此,迫切需要新的持久疾病控制战略,包括新型杀菌剂。开发新型杀菌剂的主要困难是无法确定新分子是如何杀死真菌的。在真菌酿造/烘焙酵母的模型中,已经产生了强大的工具,例如几组酵母菌株;缺乏每个真菌基因;每个蛋白质都有荧光蛋白标记,或者每个单独的蛋白质都高水平表达。这些资源使得大规模确定各种化学物质如何发挥作用来改变酵母的生物学特性成为可能。我们打算构建一套类似的完整工具,使我们能够进行这些分析,从而确定新型杀菌剂如何在小麦病原菌M.graminicola中发挥作用。具体地说,我们将把它的大约10,000个基因中的每一个放入一个可移动的遗传元件(质粒)中,这样就可以轻松地转移到其他大范围的质粒中(我们也将创建这些质粒),这些质粒可以单独或作为一个池用于灭活、荧光标记和过度表达每个基因。我们还将构建一系列禾谷分枝杆菌菌株,使我们能够确定这种天然真菌是如何入侵小麦的,我们将开发小麦感染的实验室模型来实现这一点。这将大大增加目前小麦基因组测序投资的价值。然后,我们将利用这些资源来确定杀菌剂对感染过程的影响,从而绕过新控制措施发展的主要障碍。为了确保这些资源被广泛获得,我们将为这些质粒和菌株开发一个储存库,将它们放在国际储存库中,编写标准操作程序以允许最大限度地利用这些资源,并创建一个网站来宣传和允许访问这些资源。最后,我们将与西南部(例如BBSRC North Wyke的新的综合农场平台)和全国(例如Rothamsted Research、Sainsbury实验室和先正达)的利益攸关方互动,以增加所产生工具的影响和传播。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Measurement of virulence in Zymoseptoria tritici through low inoculum-density assays.
Fluorescent markers for the Spitzenkörper and exocytosis in Zymoseptoria tritici.
  • DOI:
    10.1016/j.fgb.2015.04.014
  • 发表时间:
    2015-06
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Guo M;Kilaru S;Schuster M;Latz M;Steinberg G
  • 通讯作者:
    Steinberg G
Asynchronous development of Zymoseptoria tritici infection in wheat.
  • DOI:
    10.1016/j.fgb.2020.103504
  • 发表时间:
    2021-01
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Fantozzi E;Kilaru S;Gurr SJ;Steinberg G
  • 通讯作者:
    Steinberg G
Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.
  • DOI:
    10.1016/j.fgb.2020.103487
  • 发表时间:
    2021-01
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Fantozzi E;Kilaru S;Cannon S;Schuster M;Gurr SJ;Steinberg G
  • 通讯作者:
    Steinberg G
Construction and high-throughput phenotypic screening ofZymoseptoria tritici over-expression strains.
  • DOI:
    10.1016/j.fgb.2015.04.013
  • 发表时间:
    2015-06
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Cairns TC;Sidhu YS;Chaudhari YK;Talbot NJ;Studholme DJ;Haynes K
  • 通讯作者:
    Haynes K
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Ken Haynes其他文献

Dectin-1 is required for β-glucan recognition and control of fungal infection
Dectin-1 是β-葡聚糖识别和真菌感染控制所必需的
  • DOI:
    10.1038/ni1408
  • 发表时间:
    2006-12-10
  • 期刊:
  • 影响因子:
    27.600
  • 作者:
    Philip R Taylor;S Vicky Tsoni;Janet A Willment;Kevin M Dennehy;Marcela Rosas;Helen Findon;Ken Haynes;Chad Steele;Marina Botto;Siamon Gordon;Gordon D Brown
  • 通讯作者:
    Gordon D Brown
Value of antigen detection in predicting invasive pulmonary aspergillosis
抗原检测对侵袭性肺曲霉病的预测价值
  • DOI:
    10.1016/0140-6736(90)92831-2
  • 发表时间:
    1990
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T. R. Rogers;Ken Haynes;Rosemary Ann Barnes
  • 通讯作者:
    Rosemary Ann Barnes

Ken Haynes的其他文献

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{{ truncateString('Ken Haynes', 18)}}的其他基金

Combinatorial Responses of Fungal Pathogens To Their Human Hosts: an Integrative Systems Biology Approach
真菌病原体对其人类宿主的组合反应:综合系统生物学方法
  • 批准号:
    BB/F005210/2
  • 财政年份:
    2010
  • 资助金额:
    $ 110.48万
  • 项目类别:
    Research Grant
Modelling the transcriptional proteomic and metabolic redox regulation in Saccharomyces cerevisiae and Candida glabrata
模拟酿酒酵母和光滑念珠菌的转录蛋白质组和代谢氧化还原调节
  • 批准号:
    BB/H53146X/2
  • 财政年份:
    2010
  • 资助金额:
    $ 110.48万
  • 项目类别:
    Research Grant
Systems Approach to Biological Research Studentships
生物研究奖学金的系统方法
  • 批准号:
    BB/H531751/1
  • 财政年份:
    2010
  • 资助金额:
    $ 110.48万
  • 项目类别:
    Training Grant
Modelling the transcriptional proteomic and metabolic redox regulation in Saccharomyces cerevisiae and Candida glabrata
模拟酿酒酵母和光滑念珠菌的转录蛋白质组和代谢氧化还原调节
  • 批准号:
    BB/H53146X/1
  • 财政年份:
    2010
  • 资助金额:
    $ 110.48万
  • 项目类别:
    Research Grant
Combinatorial Responses of Fungal Pathogens To Their Human Hosts: an Integrative Systems Biology Approach
真菌病原体对其人类宿主的组合反应:综合系统生物学方法
  • 批准号:
    BB/F005210/1
  • 财政年份:
    2008
  • 资助金额:
    $ 110.48万
  • 项目类别:
    Research Grant

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    2003
  • 资助金额:
    130.0 万元
  • 项目类别:
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Understanding and Targeting the Pathophysiology of Youth-onset Type 2 Diabetes
了解并针对青年发病 2 型糖尿病的病理生理学
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