ACTIVATION OF THE HUMAN NEUTROPHIL.

这些研究旨在研究人中性粒细胞的活化， 吞噬细胞和非吞噬细胞刺激，特别是 膜脂作为刺激-反应的转换器或调节器的作用 偶合器. 钙在中性粒细胞的活化中充当“第二信使”。 胞浆Ca升高是激活后的第一个可测量事件 通过各种刺激。 Ca主要从细胞内动员 店 特别是磷脂酰肌醇分解的作用 磷脂酰肌醇4，5二磷酸与生成二酰基 甘油和肌醇1，4，5三磷酸已牵连在钙 伴随着中性粒细胞活化的运动。 这些脂质的作用， 它们在中性粒细胞活化过程中的衍生物将被严格评估。 肿瘤促进剂PMA可以绕过这种Ca需求，可能是通过 激活蛋白激酶C;该蛋白激酶C被二酰基 甘油，并作为各种钙调节钙的需求 需要酶反应。 蛋白激酶在介导和 将研究调节嗜中性粒细胞应答。 进一步 钙运动、磷脂 重塑和蛋白激酶活性应该产生重要的信息， 中性粒细胞反应的激活和调节。 以来 中性粒细胞的适当功能在宿主防御中是重要的， 在炎症性疾病中， 中性粒细胞的活化顺序具有明显的临床重要性。

These studies are designed to study the activation of human neutrophils by phagocytic and non-phagocytic stimuli, with particular reference to the role of membrane lipids as transducers or modulators of stimulus-response coupling. Calcium acts as a "second messenger" in the activation of the neutrophil. A rise in cytosolic Ca is the first measurable event following activation by a variety of stimuli. Ca is mobilized principally from intracellular stores. A role for the breakdown of phosphatidyl inositols, in particular phosphatidyl inositol 4,5 bisphosphate with the generation of diacyl glycerol and inositol 1,4,5 trisphosphate has been implicated in the Ca movements accompanying neutrophil activation. The role of these lipids and their derivatives during neutrophil activation will be critically evaluated. The tumor promoter PMA can bypass this Ca requirement, possibly by activating a protein kinase C; this protein kinase C is activated by diacyl glycerol and serves to modify the Ca requirements as a variety of Ca requiring enzyme reactions. The role of protein kinases in mediating and modulating the neutrophil responses will be investigated. Further investigation into the inter-relation of Ca movements, phospholipid remodeling and protein kinase activity should yield crucial information on the activation and modulation of neutrophil responses. Since the appropriate functioning of the neutrophil is important in host defenses, as well as in inflammatory disorders, a more fundamental understanding of the activation sequence of the neutrophil is of obvious clinical importance.