RECOMBINATION IN THE MULTIPARTITE RNA VIRUSES

多部分 RNA 病毒中的重组

• 批准号: 3140704
• 负责人: Jozef Julian Bujarski
• 金额: $ 10.71万
• 依托单位: NORTHERN ILLINOIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3140704
• 关键词: Bromovirus; Comovirus; RNA biosynthesis; RNA virus; biochemical evolution; chimeric proteins; gel electrophoresis; gene deletion mutation; genetic crossing over; genetic markers; genetic recombination; genome; messenger RNA; nucleic acid sequence; oligonucleotides; point mutation; temperature sensitive mutant; tobacco mosaic virus; transposon /insertion element; virus RNA; virus genetics; virus infection mechanism

The overall goal of our research project is to understand how virus-encoded information determines infectivity, host range and other interactions of mRNA viruses with their hosts. We are particularly interested in the molecular mechanisms by which recombination processes between genomic RNAs provide these viruses with some special means for their flexibility and rapid evolution. In this project we will contribute to the understanding of recombination processes in multipartite mRNA viruses. We will study three closely related model bromoviruses: brome mosaic virus, cowpea chlorotic mottle virus and broad bean mottle virus. By using various bromoviral RNA mutants, we will investigate recombination in whole plants and in plant protoplasts. Homologous recombination between various bromoviral RNA components will be studied using infectious mutations in both coding and noncoding regions. To investigate sequence and structural factors which determine recombination, the sites of recombination will be localized over bromoviral RNA genome and sequences around these sites will be characterized. Experiments will be undertaken to elucidate at what stage of RNA synthesis the recombination occurs. The primary importance of this work lies in its impact on fundamental virology and biology; the results are directly applicable for other RNA viruses infecting both animals and plants.

我们研究项目的总体目标是了解病毒是如何编码的 信息决定了传染性、宿主范围和其他相互作用 信使核糖核酸病毒与它们的宿主。我们特别感兴趣的是 基因组RNA间重组过程的分子机制 为这些病毒提供了一些特殊的手段来实现它们的灵活性和 快速进化。 在这个项目中，我们将有助于理解重组 在多部分信使核糖核酸病毒中的过程。我们将仔细研究三个方面 相关模式雀麦花叶病毒、豌豆褪绿斑病毒 病毒和蚕豆斑驳病毒。通过使用各种溴病毒RNA突变体， 我们将研究整个植物和植物原生质体中的重组。 不同溴病毒RNA成分之间的同源重组将是 使用编码区和非编码区的感染性突变进行了研究。 调查决定序列和结构的因素 重组，重组位点将定位于新冠病毒之上。 RNA基因组和这些位点周围的序列将被描述。 将进行实验以阐明rna合成的哪个阶段。 重组就会发生。 这项工作的首要意义在于它对根本的影响 病毒学和生物学；结果直接适用于其他RNA 感染动植物的病毒。